Abstract
In the long-term cultivated callus cultures ofMatricaria recutita L. the identical concentration changes in the biosynthesis of glutathione, glutamate, aspartate, total thiols and proteins were detected within the subculture. The level of oxidized glutathione during the growth of callus culture was low with the highest value 10.66 nmol g-1 on the 13th day of subculture. The ratio GSH/GSSG which significantly influences the redox processes in a cell, and the activity of glutathione reductase increased from the 8th day. Ascorbate formation was detected on the 17th day, although no relation between the ascorbate synthesis and the concentration of glutathione and glutathione reductase was found.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bergmann, L., Rennenberg, H.: Efflux und Produktion von Glutathion in Suspensionskulturen von Nicotianatabacum. — Z. Pflanzephysiol.88:175–185, 1978.
Fahey, R. C, Di Stefano, D. L., Meier, D. L., Bryan, R. N.: Role of hydratation state and thiol-disulfide status in the control of thermal stability and protein synthesis in wheat embryo. — Plant Physiol.65:1062–1066, 1980.
Gamborg, O. L., Miller, R. A., Ojima, K.: Nutrient requirements in suspension culture of soybean root cells. — Exp. Cell Res.50: 151–158, 1968.
Haluwell, B.: Chloroplast Metabolism: The Structure and Function of Chloroplast in Green Leaf Cells. — Clarendon Press, Oxford 1984.
Haluwell, B., Foyer, C. H.: Properties and physiological function of glutathione reductase purified from spinach leaves by affinity chromatography. — Planta139: 9–17, 1978.
Jablonski, P. P., Anderson, J. W.: Light dependent reduction of dehydroascorbate by ruptured pea chloroplast. — Plant Physiol.67: 1239–1244, 1981.
Kosower, N. S., Kosower, E. M.: Functional aspects of glutathione. — In:Arias, J. M., Jakoby, W. B. (ed.): Glutathione: Metabolism and Function. Pp. 159–174. Raven Press, New York 1976.
Lamourex, G. L.,Rusness, D. G.: Catabolism of glutathione conjugates of pesticides in higher plants. — In:Rosen, J. D.,Magee, P. S.,Casida, J. E. (ed): Sulphur and Pesticide Action and Metabolism. Pp. 132–164. ACS Symp. Ser. 152, 1981.
Lamourex, G. L., Rusness, D. G.: Coenzymes and Cofactors. — J. Wiley, New York 1987.
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, J.: Protein measurement with the Folin phenol reagent. — J. biol. Chem.193: 265–275, 1951.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bloassays with tobacco tissue cultures. -Physiol. Plant.15: 473–497, 1962.
Reed, J., Babson, J. R., Beatfy, P. W., Brodie, A. E., Ellis, W. W., Potfer, W.: High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulphide, and related thiols and disulphides. - Anal. Biochem.106: 55–62, 1980.
Sedlák, J., Lidsay, R.: Estimation of total protein-bound and nonprotein sulphydryl groups in tissue with Ellman’s reagent. - Anal. Biochem.25: 192–205, 1968.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Podhradský, D., Ĉellárová, E. & HonĈariv, R. Oxidized and reduced glutathione, ascorbate and glutathione reductase inmatricaria recutita L. Callus cultures. Biol Plant 32, 81–88 (1990). https://doi.org/10.1007/BF02897543
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02897543