Folia Microbiologica

, Volume 25, Issue 6, pp 501–504 | Cite as

Preparation of zymosan from yeast cell walls

  • Z. Holan
  • K. Beran
  • I. Miler
Brief Reports


Cell walls of the yeastSaccharomyces cerevisiae after disintegration and protoplasm removal by centrifugation and repeated washing were suspended in 0.5m Na2HPO4, pH 7.8–8.0, as a 5% or 10% suspension, depending on the mode of heating. The suspension was boiled for 3h, purified by repeated washing with water and ethanol and dried. The yield was approximately 1.8% of the starting amount of pressed commercial baker’s yeast.


Glucan Yeast Cell Wall Glucomannan Murine Mammary Tumor Murine Mammary Carcinoma 
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Copyright information

© Academia, Publishing House of the Czechoslovak Academy of Sciences 1980

Authors and Affiliations

  • Z. Holan
    • 1
  • K. Beran
    • 1
  • I. Miler
    • 2
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4
  2. 2.Research Institute for the Care of Mother and ChildPrague 4

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