Preparation of zymosan from yeast cell walls
Cell walls of the yeastSaccharomyces cerevisiae after disintegration and protoplasm removal by centrifugation and repeated washing were suspended in 0.5m Na2HPO4, pH 7.8–8.0, as a 5% or 10% suspension, depending on the mode of heating. The suspension was boiled for 3h, purified by repeated washing with water and ethanol and dried. The yield was approximately 1.8% of the starting amount of pressed commercial baker’s yeast.
KeywordsGlucan Yeast Cell Wall Glucomannan Murine Mammary Tumor Murine Mammary Carcinoma
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