Folia Microbiologica

, Volume 25, Issue 6, pp 501–504 | Cite as

Preparation of zymosan from yeast cell walls

  • Z. Holan
  • K. Beran
  • I. Miler
Brief Reports


Cell walls of the yeastSaccharomyces cerevisiae after disintegration and protoplasm removal by centrifugation and repeated washing were suspended in 0.5m Na2HPO4, pH 7.8–8.0, as a 5% or 10% suspension, depending on the mode of heating. The suspension was boiled for 3h, purified by repeated washing with water and ethanol and dried. The yield was approximately 1.8% of the starting amount of pressed commercial baker’s yeast.


Glucan Yeast Cell Wall Glucomannan Murine Mammary Tumor Murine Mammary Carcinoma 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Balitskii K.P., Veksler I.G.: Increase in the antitumor effect of alkylating agents with the help of action on the protective reactions of the connective tissue system. (In Russian).Tr. Kaz. Nauch. Issled. Inst. Onkol. Radiol. 3, 291 (1967);Chem. Abstr. 68, 103 774 p (1968).Google Scholar
  2. Beran K., Řeháček J., Ott J., Bičík V., Kašpar J.: Production of biologically active compounds by extraction.Czech. Pat.,130 926 (1968).Google Scholar
  3. Bradner W.T., Clarke D.A., Stock C.C.: Stimulation of host defence against experimental cancer. I. Zymosan and sarkoma 180 in mice.Cancer Res. 18, 347 (1958).PubMedGoogle Scholar
  4. DiCarlo F.J., Fiore J.V.: Composition of zymosan.Science 127, 756 (1958).CrossRefGoogle Scholar
  5. Diller J.C., Mankowski Z.T.: Response of sarkoma 37 and normal cells of the mouse host to zymosan and hydroglucan.Acta Unio. Intern. Contra Cancrum 16, 584 (1960).Google Scholar
  6. Fencl Z., Machek F., Šilinger V.: Method of the preparation of native microbial protein with a low content of nucleic acid, applicable as food or fodder.US Pat. 4 007 088 (1977).Google Scholar
  7. Fugmann R.A., Martin D.S., Hayworth P.E., Stolfi R.L.: Enhanced cures of spontaneous murine mammary carcinomas with surgery and fine-compound combination chemotherapy and their immunotherapeutic interrelation.Cancer Res. 30, 1931 (1970).PubMedGoogle Scholar
  8. Holan Z., Beran K., Miler I.: Zymosan preparation from the waste after the production of yeast proteins.Czech. Pat. 185 063 (1978).Google Scholar
  9. Isliker H., Leuchtenberger C., Kliem U.: Effects of prolonged RES stimulation on the frequency of malignant tumors and lymphomatous disorders in mice.J. Reticuloendothel. Soc. 13, 459 (1973).PubMedGoogle Scholar
  10. Latii N.P., Konstantinov V.V.: Effect of zymosan on the state of nonspecific immunological reactivity of the body during surgical radiation treatment of malignant neoplasms in an experiment.Onkologia (Kiev)1, 85 (1970).Google Scholar
  11. Lührs W., Heise E., Berndt H.: Influence of zymosan on experimental animal tumor growth.Z. Krebsforsch. 62, 516 (1958).PubMedCrossRefGoogle Scholar
  12. Maeda Y.Y., Chihara G., Ishimura K.: Unique increase of serum proteins and action of antitumor polysaccharides.Nature 252, 250 (1974).PubMedCrossRefGoogle Scholar
  13. Mankowski Z.T., Yamashita M., Diller I.C.: Effect ofCandida guilliermondi polysaccharide on transplantable mouse sarkoma 37.Proc. Soc. Exp. Biol. Med. 96, 79 (1957).PubMedGoogle Scholar
  14. Martin D.S., Hayworth P.E., Fugmann R.A.: Enhanced cures of spontaneous murine mammary tumors with surgery, combination chemotherapy and immunotherapy.Cancer Res. 30, 709 (1970).PubMedGoogle Scholar
  15. Pfordte K., Ponsold W.: Growth of transplantable tumors in animals during combined treatment with cytostatics and yeast cell wall polysaccharide zymosan.Neoplasma 18, 173 (1971).PubMedGoogle Scholar
  16. Pillemer L., Blum L., Lepow I.H., Wurz L., Todd E.W.: The properdin system and immunity. III. The zymosan assay of properdin.J. Exp. Med. 103, 1 (1956).PubMedCrossRefGoogle Scholar
  17. Sokoloff B.: Experimental studies on mitomycin C. IV. Zymosan and the reticuloendothelial system.Growth 25, 249 (1961).PubMedGoogle Scholar
  18. Veksler I.G.: Effect of zymosan and prodigiosan on the growth and metastasis of experimental tumors.Chem. Abstr. 81, 1148ly (1974).Google Scholar
  19. Yamabayshi H.: Zymosan like substance extracted fromCandida albicans.Med. J. Osaka Univ. 9, 11 (1958).Google Scholar

Copyright information

© Academia, Publishing House of the Czechoslovak Academy of Sciences 1980

Authors and Affiliations

  • Z. Holan
    • 1
  • K. Beran
    • 1
  • I. Miler
    • 2
  1. 1.Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4
  2. 2.Research Institute for the Care of Mother and ChildPrague 4

Personalised recommendations