Developmental expression of myelin proteolipid, basic protein, and 2′,3′-cyclic nucleotide 3′-phosphodiesterase transcripts in different rat brain regions
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RNA was extracted from five different rat brain regions during development, starting from embryonic day 15 (E15) until postnatal day 60 (P60). These RNA preparations were analyzed by both Northern and dot blot for their content of 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase), myelin proteolipid protein (PLP), and myelin basic protein (MBP) -specific transcripts. CNPase mRNA was readily detectable at E15 and PLP mRNA at P1 in all brain regions examined. In contrast, expression of MBP mRNA followed a caudorostral gradient. It was first observed at P1 in the mesencephalon and at P9-P11 in the olfactory bulb. Expression of these three transcripts displayed two types of developmental profiles. One was termed biphasic because the specific mRNA level increased regularly and then reached a plateau level. The other developmental profile was termed triphasic, because there was a gradual increase in the level of specific transcripts with a sudden appearance of a sharp peak followed by a decline to a plateau level. When the triphasic pattern was observed, the date of the peak appearance was probe-, but not region-, dependent. It was P15 for CNPase, P18 for MBP, and P21 for PLP. As these peaks occurred at a time during development when myelination was the most active, we postulate the existence of a transient external signal, perhaps neuronal, which would be responsible for this increased amount of myelin-related transcripts.
KeywordsOlfactory Bulb Myelin Protein Developmental Expression Developmental Profile Myelin Proteolipid Protein
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- Anderson, M.L.M., Young, B.D. (1985) Quantitative filter hybridisation. Nucleic Acid Hybridisation, A Practical Approach B.D. Hames, S.J. Higgins (eds). IRL Press, Oxford, pp 73–113Google Scholar
- Bernier, L., Alvarez, F., Norgard, E.M., Raible, D.W., Mentaberry, A., Shemri, J.G., Sabatini, D.D., Colman, D.R. (1987) Molecular cloning of a 2′,3′-cyclic nucleotide 3′-phosphohydrolase: mRNAs with different 5′ ends encode the same set of proteins in nervous and lymphoid tissues. J. Neurosci. 7:2703–2710PubMedGoogle Scholar
- Civelli, O., Birnberg, N, Herbert, E. (1982) Detection and quantification of pro-opiomelanocortine mRNA in pituitary and brain tissues from different species. J. Biol. Chem. 255:6782–6792Google Scholar
- Delassalle, A., Zalc, B., Lachapelle, F., Raoul, M., Collier, P., Jacque, C. Regional distribution of myelin basic protein in the CNS of quaking, jimpy and normal mice during development and aging. J Neurosci. Res. 6:303–313Google Scholar
- Faucon-Biguet N., Buda M., Lamouroux A., Samolyk D., Mallet J. (1986) Time course of the changes of TH mRNA in rat brain and adrenal medulla after single injection of reserpine. EMBO J. 5:287–291Google Scholar
- Paterson, J.A., Privat, A., Ling, E.A., Leblond, C.P. (1973) Investigation of glial cells in semithin sections III. Transformation of subependymal cells into glial cells, as shown by radioautography after3H thymidine injection into the lateral ventricle of the brain of young rats. J. Comp. Neurol. 149:83–102PubMedCrossRefGoogle Scholar
- Yakovlev, P.I., Lecours, A.R. (1967) The myelogenetic cycles of regional maturation of the brain. Regional Developments of the Brain in Early Life. Minkowski (ed). Blackwell Scientific, Oxford, pp 3–71Google Scholar