Virchows Archiv B

, Volume 55, Issue 1, pp 355–361 | Cite as

Oncogene expression in endocrine pancreatie tumors

  • Heinz Höfler
  • Christine Ruhri
  • Barbara Pütz
  • Gerhard Wirnsberger
  • Hubert Häuser


The mRNA expression of the (proto) oncogenes Ha-ras, Ki-ras, fos, c-myc, N-myc, and sis was studied in five pancreatic endocrine tumors and two non-neoplastic pancreases by in situ hybridization and Northern blot analysis. Ha-ras, Ki-ras, fos and c-myc, but not N-myc or sis mRNA was detected in all the tumors as well as in the non-neoplastic pancreatic tissues. Compared with non-tumorous pancreatic tissue, Ha-ras and Ki-ras mRNA was overexpressed up to 42-fold in all the tumors; metastasizing tumors showed 2–6 times higher Ha-ras mRNA levels than benign neoplasias. In contrast, c-myc mRNA levels were higher in normal tissue than in tumors and fos mRNA levels did not differ significantly between tumors and normal tissue. The activities of Ki-ras, fos and c-myc mRNA expression did not correlate with any of the histological or biological properties of the tumors, nor with the clinical course of disease. Our results, although based on a limited number of cases, suggest that Ha-ras and Ki-ras mRNA overexpression is associated with the development of pancreatic endocrine tumors. The measurement of Ha-ras mRNA levels may contribute to the assessment of tumor prognosis.

Key words

Endocrine pancreatic tumors Oncogenes mRNA Hybridization 


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Heinz Höfler
    • 1
  • Christine Ruhri
    • 1
  • Barbara Pütz
    • 1
  • Gerhard Wirnsberger
    • 1
  • Hubert Häuser
    • 1
  1. 1.Division of Oncology and Experimental Cell Research, Institute of PathologyUniversity of Graz School of MedicineGrazAustria

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