Oncogene expression in endocrine pancreatie tumors
- 10 Downloads
The mRNA expression of the (proto) oncogenes Ha-ras, Ki-ras, fos, c-myc, N-myc, and sis was studied in five pancreatic endocrine tumors and two non-neoplastic pancreases by in situ hybridization and Northern blot analysis. Ha-ras, Ki-ras, fos and c-myc, but not N-myc or sis mRNA was detected in all the tumors as well as in the non-neoplastic pancreatic tissues. Compared with non-tumorous pancreatic tissue, Ha-ras and Ki-ras mRNA was overexpressed up to 42-fold in all the tumors; metastasizing tumors showed 2–6 times higher Ha-ras mRNA levels than benign neoplasias. In contrast, c-myc mRNA levels were higher in normal tissue than in tumors and fos mRNA levels did not differ significantly between tumors and normal tissue. The activities of Ki-ras, fos and c-myc mRNA expression did not correlate with any of the histological or biological properties of the tumors, nor with the clinical course of disease. Our results, although based on a limited number of cases, suggest that Ha-ras and Ki-ras mRNA overexpression is associated with the development of pancreatic endocrine tumors. The measurement of Ha-ras mRNA levels may contribute to the assessment of tumor prognosis.
Key wordsEndocrine pancreatic tumors Oncogenes mRNA Hybridization
Unable to display preview. Download preview PDF.
- Aaronson StA, Tronick StR (1986) The role of oncogenes in human neoplasias. In: De Vita VT, Hellman S, Rosenberg S (eds) Important advances in oncology 1986. JB Lippincott Co, PhiladelphiaGoogle Scholar
- Barbacid M (1986) Human oncogenes. In: De Vita VT, Hellman S, Rosenberg S (eds) Important advances in oncology 1986. JB Lippincott Co, PhiladelphiaGoogle Scholar
- Buick RN, Pollak MN (1984) Perspectives on clonogenic tumor cells, stem cells, and oncogenes. Cancer Res 44:4909^918Google Scholar
- Höfler H, Mobtaker H, Carney WP, Childers H, DeLellis RA, Tischler AS, Wolfe HJ (1986 b) Simultaneous localization of Ha ras mRNA and protein p21 by in situ hybridization with antisense RNA probes and immunohistochemistry. Lab Invest 54/1:26 AGoogle Scholar
- Höfler H, Tischler AS, DeLellis RA, Merk F, Wolfe HJ (1988) Increased expression of c-fos oncogene mRNA after depolarization of diverse neuroendocrine cell types. Lab Invest 56:39AGoogle Scholar
- Klimpfinger M, Ruhri Ch, Pütz B, Pfragner R, Wirnsberger G, Höfler H (1988) Oncogene expression in a medullary thyroid carcinoma. Virchows Arch [Cell Pathol] 54/4:256–259Google Scholar
- Maniatis T, Fritsch EF, Sambrock J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor LaboratoryGoogle Scholar
- Riou G, Barrois M, Le MG, George M, Le Doussal V, Haie Ch (1987) C-myc proto-oncogene expression and prognosis in early carcinoma of the uterine cervix. Lancet 761–764Google Scholar
- Shimuzu K, Goldfarb M, Suard Y, Perucho M, Li Y, Kamata T, Feramisco J, Stavnezer RE, Fogh J, Wigler M (1983) Three human transforming genes are related to the viral ras oncogenes. Proc Natl Acad Sci USA 80:3–7Google Scholar
- Stacey DW, Smith MR (1986) Cellular ras proteins and proliferative signal transduction: In Eisenman R, Skalka AM (eds) Normal and tumor cells. In RNA tumor viruses. Cold Spring Harbor Laboratory, New York, p 229 ffGoogle Scholar