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Virchows Archiv B

, Volume 55, Issue 1, pp 293–298 | Cite as

Extracellular matrix of cultivated, poorly differentiated human gastric adenocarcinoma cells promotes attachment and spreading of mesenchymal cells

  • Scigou Akao
  • Mitsuko Sobue
  • Toshiaki Fukatsu
  • Tetsuro Nagasaka
  • Nobuo Nakashima
  • Jun Takeuchi
Article

Summary

To clarify interactions between carcinoma and mesenchymal cells, we examined the extracellular matrix-substance remaining on culture dishes after confluent growths of gastric carcinoma cells were removed with EDTA. The matrix synthesized by poorly differentiated adenocarcinoma cells (cell lines KATO-III and MKN-45) cultivated in serum-free medium has a fibroblast (cell line WI38)-attachment activity, which is not detected in the matrix synthesized by a well differentiated adenocarcinoma (cell line MKN-28). This activity was not observed in KATO-III-matrix extracted with 6 M urea, but could be detected in a 1% SDS extract from the remaining matrix on the culture dishes after 6 M urea extraction. The activity was abolished by treatment with pronase (16(μg/ml), trypsin (0.005%) or alkali, but was unaffected by collagenase (80 μg/ml, 4 h) or chondroitinase ABC (1 U/ml, 1 h). It is conceivable that the fibroblastattachment activity of the matrix produced by poorly differentiated adenocarcinoma cells is related to the proliferation of interstitial connective tissue in vivo.

Key words

Fibroblast attachment Matrix of carcinoma cells Gastric cancer 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Scigou Akao
    • 1
  • Mitsuko Sobue
    • 1
  • Toshiaki Fukatsu
    • 2
  • Tetsuro Nagasaka
    • 1
  • Nobuo Nakashima
    • 2
  • Jun Takeuchi
    • 1
  1. 1.Department of Laboratory MedicineNagoya University School of MedicineNagoyaJapan
  2. 2.Division of PathologyNagoya University HospitalNagoyaJapan

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