Summary
To clarify interactions between carcinoma and mesenchymal cells, we examined the extracellular matrix-substance remaining on culture dishes after confluent growths of gastric carcinoma cells were removed with EDTA. The matrix synthesized by poorly differentiated adenocarcinoma cells (cell lines KATO-III and MKN-45) cultivated in serum-free medium has a fibroblast (cell line WI38)-attachment activity, which is not detected in the matrix synthesized by a well differentiated adenocarcinoma (cell line MKN-28). This activity was not observed in KATO-III-matrix extracted with 6 M urea, but could be detected in a 1% SDS extract from the remaining matrix on the culture dishes after 6 M urea extraction. The activity was abolished by treatment with pronase (16(μg/ml), trypsin (0.005%) or alkali, but was unaffected by collagenase (80 μg/ml, 4 h) or chondroitinase ABC (1 U/ml, 1 h). It is conceivable that the fibroblastattachment activity of the matrix produced by poorly differentiated adenocarcinoma cells is related to the proliferation of interstitial connective tissue in vivo.
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Akao, S., Sobue, M., Fukatsu, T. et al. Extracellular matrix of cultivated, poorly differentiated human gastric adenocarcinoma cells promotes attachment and spreading of mesenchymal cells. Virchows Archiv B Cell Pathol 55, 293–298 (1988). https://doi.org/10.1007/BF02896588
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DOI: https://doi.org/10.1007/BF02896588