Indian Journal of Clinical Biochemistry

, Volume 11, Issue 2, pp 168–170 | Cite as

Blast transformation of lymphocytes stimulated with phytohaemagglutinin in dilated cardiomyopathy (DCM) patients

  • R. L. Tripathi
  • K. K. Talwar
  • K. S. S. Saibaba
  • L. M. Srivastava
Brief Communications


Blast transformation of peripheral blood lymphocytes (PBL) in dilated cardiomyopathy (DCM) patients and normal controls was examined to assess the cell mediated immune response of these patients.

Uptake of3H-thymidine by PBL of DCM patients incubated for 72 hrs with phytohaemagglutinin (PHA) was 17787±9513 CPM and without PHA was 1475±529 CPM showing a stimulation index (SI) by PHA of 12.95±6.35. The corresponding values in healthy controls were 55078±12282 and 1868±792 respectively with a SI of 38.0±26.0. Blast transformation of PBL by PHA was significantly suppressed in DCM patients as compared to normal controls. This suppression was found to be highly significant and possibly indicates the defective cell medicated immune response in DCM patients.

Key words

Dilated cardiomyopathy stimulating index blast transformation peripheral blood lymphocytes (PBL) 


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Copyright information

© Association of Clinical BIochemists of India 1996

Authors and Affiliations

  • R. L. Tripathi
    • 1
  • K. K. Talwar
    • 2
  • K. S. S. Saibaba
    • 4
  • L. M. Srivastava
    • 3
  1. 1.In Service Training Programme MLT Unit & Hospital Laboratory ServicesDeptt. of Biochemistry UCMS & G.T.B. HospitalDelhi
  2. 2.Department of CardiologyAll India Institute of Medical SciencesNew Delhi
  3. 3.Department of BiochemistryAll India Institute of Medical SciencesNew Delhi
  4. 4.Department of BiochemistrySri Venkateswara Institute of Medical SciencesTirupati

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