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IL-5 up-regulates the expression of TGF-β1 in human blood eosinophilsin vitro

  • Huang Yabing
  • Liu Bin
  • Wang Lu
  • Li Rong
  • Zhu Min
  • Chen Dong
  • Chen Shi
Article

Summary

To investigate the effects of IL-5 on the expression of TGF-β1 in eosinophilsin vitro, eosinophils were incubated in the presence of the same concentrations of IL-4, IL-5 and IFNγ, different concentrations of IL-5 in vitro and changes of eosinophil viability were assessed by trypan blue exclusion. Non-cytokine was employed as a negative control. 16 h after the cultivation, supernatants and cells were assayed by using TGF-β1 specific ELISA and RT-PCR. The mRNA expression and protein expression of TGF-β1 in eosinophils stimulated with different cytokines was observed. The expression of TGF-β1 protein in eosinophils was increased significantly by IL-4 (433.67±9.86 vs 228.9±2.87) and IL-5 (403.72±7.60 vs 228.9±2.87,P<0.05), while decreased by IFNγ (178.47±2.60 vs 228.9±2.87). At the same time, the results demonstrated that the basal level of TGF expression was enhanced by IL-5 in all samples (P<0.05). The expression of TGF-β1 mRNA was 1.42, 1.70, 1.76-folds higher than that of the non-stimulated controls. It is concluded that IL-5 can up-regulate the expression of TGF-β1 in eosinophilsin vitro, which might have effect in eosinophil-associated chronic rejection.

Key words

IL-5 transforming growth factor-betal (TGF-β1) 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Huang Yabing
    • 1
    • 2
  • Liu Bin
    • 1
    • 2
  • Wang Lu
    • 1
    • 2
  • Li Rong
    • 1
    • 2
  • Zhu Min
    • 1
    • 2
  • Chen Dong
    • 1
    • 2
  • Chen Shi
    • 1
    • 2
  1. 1.Institute of Organ Transplantation, Tongji Hospital, Tongji Medical CollegeHuzhong University of Science and TechnologyWuhanChina
  2. 2.Key Laboratory of Organ TransplantationMinistry of Education and HealthWuhanChina

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