Summary
To investigate the effects of IL-5 on the expression of TGF-β1 in eosinophilsin vitro, eosinophils were incubated in the presence of the same concentrations of IL-4, IL-5 and IFNγ, different concentrations of IL-5 in vitro and changes of eosinophil viability were assessed by trypan blue exclusion. Non-cytokine was employed as a negative control. 16 h after the cultivation, supernatants and cells were assayed by using TGF-β1 specific ELISA and RT-PCR. The mRNA expression and protein expression of TGF-β1 in eosinophils stimulated with different cytokines was observed. The expression of TGF-β1 protein in eosinophils was increased significantly by IL-4 (433.67±9.86 vs 228.9±2.87) and IL-5 (403.72±7.60 vs 228.9±2.87,P<0.05), while decreased by IFNγ (178.47±2.60 vs 228.9±2.87). At the same time, the results demonstrated that the basal level of TGF expression was enhanced by IL-5 in all samples (P<0.05). The expression of TGF-β1 mRNA was 1.42, 1.70, 1.76-folds higher than that of the non-stimulated controls. It is concluded that IL-5 can up-regulate the expression of TGF-β1 in eosinophilsin vitro, which might have effect in eosinophil-associated chronic rejection.
Similar content being viewed by others
References
Del Prete G, Maggi E, Romagnani S. Human Th1 and Th2 cells: functional properties, mechanisms of regulation, and role in disease. Lab invest, 1994, 70(3): 299
Honjo K, Xu X Y, Bucy R P. Heterogeneity of T cell clones specific for a single indirect alloantigenic epitope (I-Ab/H-2Kd54-68) that mediate transplant rejection. Transplantation, 2000, 70(10): 1516
Lai K F, Guo X M, Wang C Zet al. An improved technique for the isolation and culture of eosionphils of normal human beings. J Third Milit Med Univer, 1999, 21(10): 763
Foster P F, Sankary H N, Hart Met al. Blood and graft eosinophilia as predictors of rejection in human liver transplantation. Transplantation, 1989, 47(1): 72
Hongwei W, Nanra R S, Stein Aet al. Eosinophils in acute renal allograft rejection. Transpl. Immunol, 1994, 2(1): 41
Goldman M, Le Moine A, Braun Met al. A role for eosinophils in transplant rejection. Trends Immunol, 2001, 22(5): 247
O'Donnell M P. Renal tubulointerstitial fibrosis. New thoughts on its development and progression. Postgrad Med, 2000, 108(1): 159
Le Moine A, Flamand V, Demoor F Xet al. Critical roles for IL-4, IL-5, and eosinophils in chronic skin allograft rejection. J Clin Invest, 1999, 103(12): 1659
Author information
Authors and Affiliations
Additional information
HUANG Yabing, female, born in 1978, Postgraduate Student
This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30200263).
Rights and permissions
About this article
Cite this article
Yabing, H., Bin, L., Lu, W. et al. IL-5 up-regulates the expression of TGF-β1 in human blood eosinophilsin vitro . J. Huazhong Univ. Sci. Technol. [Med. Sci.] 25, 665–667 (2005). https://doi.org/10.1007/BF02896165
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02896165