Abstract
Sonicated cells ofEscherichia coli contain an enzyme system degrading 5′ adenosine monophosphate (5′ AMP) to hypoxanthine. This enzyme system is located in the fraction sedimenting at 20,000 xg. It has a pH optimum at 8.0. In the fraction sedimenting at 20,000 xg the enzyme activity was inhibited by adenosine triphosphate (ATP). Adenosine and adenine are deaminated by this enzyme preparation to inosine and to hypoxanthine, these activities not being inhibited by ATP.
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Abbreviations
- 5′AMP:
-
5′ adenosine monophosphate
- Ado-3′, 5′-P:
-
cyclic 3′, 5′-adenosine monophosphate
References
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Braná, H. Degradation of 5′ adenosine monophosphate in a cell-free system ofEscherichia coli . Folia Microbiol 12, 1–5 (1967). https://doi.org/10.1007/BF02895083
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DOI: https://doi.org/10.1007/BF02895083