Folia Microbiologica

, Volume 12, Issue 1, pp 1–5 | Cite as

Degradation of 5′ adenosine monophosphate in a cell-free system ofEscherichia coli

  • H. Braná


Sonicated cells ofEscherichia coli contain an enzyme system degrading 5′ adenosine monophosphate (5′ AMP) to hypoxanthine. This enzyme system is located in the fraction sedimenting at 20,000 xg. It has a pH optimum at 8.0. In the fraction sedimenting at 20,000 xg the enzyme activity was inhibited by adenosine triphosphate (ATP). Adenosine and adenine are deaminated by this enzyme preparation to inosine and to hypoxanthine, these activities not being inhibited by ATP.


Adenosine Adenine Bran Enzyme Preparation Ribose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used


5′ adenosine monophosphate

Ado-3′, 5′-P

cyclic 3′, 5′-adenosine monophosphate


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  1. Braná, H., Chytil, F.:Splitting of the cyclic 3′5′-adenosine monophosphate in cell-free system of Escherichia coli. Fol. microbiol. 11: 43, 1966.CrossRefGoogle Scholar
  2. Butcher, R. W., Sutherland, E. W.:Adenosine 3′5′-phosphate in biological materials. I. Purification and properties of cyclic 3′5′-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3′5′-phosphate in human urine. J. biol. Chem. 237: 1244, 1962.PubMedGoogle Scholar
  3. Lehman, I. R., Bessman, M. J., Simms, E. S., Kornberg, A.:Enzymatic synthesis of DNA. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. J. biol. Chem. 233: 163, 1958.PubMedGoogle Scholar
  4. Lowry, O. H., Lopez, J. A.:The determination of inorganic phosphate with presence of labile phosphate esters. J. biol. Chem. 193: 265, 1951.PubMedGoogle Scholar
  5. Meijbaum, W. Z.:Über die Bestimmung kleiner Pentosemengen, insbesonders in Derivaten der Adenylsäure. Ztschr. Physiol. Chem. 258: 117, 1939.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1967

Authors and Affiliations

  • H. Braná
    • 1
  1. 1.Department of Genetics and Variability of Microorganisms, Institute of MicrobiologyCzechoslovak Academy of SciencesPrague 4

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