Abstract
This paper deals with some morphological and histological changes observed during regular intervals inMatricaria inodora L. tissue cultures derived from leaf expiants. The expiants were cultivated on Murashige-Skoog’s culture medium supplemented with 1.0 mg 1−1 2,4-dichlorophenoxyacetic acid. The callus formation started about a week after isolation. During the second week the meristematic centers were differentiated from which root and shoot apices were later formed. During long term cultivation under the same culture conditions the inhibition of development of shoot apices took place. Only roots of unorganized growth have been regenerated. The influence of culture conditions on morphogenetic potential is discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Butenko, R. G.: Eksperimentalnyï Morfogenez i Differentsiyatsiya v Kulture Kletok Rasteniï. [Experimental Morphogenesis and Differentiation in Plant Cell Culture.] - Nauka, Moskva 1975.
Čellárová, E., Grelákova, K., Repčák, M., Hončariv, R.: Morphogenesis in callus tissue cultures of someMatricaria andAchillea species. - Biol. Plant.24: 430–433, 1982.
Fosket, D. E.: Hormonal control of morphogenesis in cultured tissues. - In:Skoog, F. (ed.): Plant Growth Substances 1979. (Proc. 10th Int. Conf. on Plant Growth Substances.) Pp. 362 to 369. Madison, Wisconsin 1979.
Luštinec, J., Horák, J.: Induced regeneration of plants in tissue cultures ofBrassica oleracea. - Experientia26: 919–920, 1970.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. -Physiol. Plant.15: 473–479, 1962.
Němec, B. et al.: Botanická Mikrotechnika. [Botanical Microtechnique.] - Nakl. ČSAV, Praha 1962.
Novák, F. J., Opatrný, Z., Rovenská, B., Neštický, M.: Studies on the morphogenetic response of maize tissue cultures of different origin. - Biol. Plant.21: 418–426, 1979.
Raju, M. V. S., Mann, H. E.: Regenerative studies on the detached leaves ofEcheveria elegans. Anatomy and regeneration of leaves in sterile culture. - Can. J. Bot.48: 1887–1891, 1970.
Reinert, J., Bajaj, Y. P. S. (ed.): Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture.- Springer Verlag, Berlin-Heidelberg-New York 1977.
Shimada, T.: Plant regeneration from stem-derived calluses of wheat. - Wheat Information Service Kihara Institute for Biological Research, Yokohama. Pp. 59–60. Yokohama 1979.
Shimada, T., Yamada, Y.: Wheat plants regenerated from embryo cell cultures. - Jap. J. Genet.51: 379–385, 1979.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rychlová, M., Čellárová, E. & Hončariv, R. The study of morphological and histological changes in tissue cultures ofmatricaria inodora L. Biol Plant 26, 197–201 (1984). https://doi.org/10.1007/BF02895049
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02895049