Abstract
The growth ofArabidopsis thaliana calli on media without growth regulators was studied Calli under study showed autonomy for cytokmms independently whether cultivated on the light or m the dark When cultivated in intense light, they were able to grow, either transiently or permanently, on the medium without any growth regulators In the dark, they were strictly dependent on 2,4 D m the medium Both the intensity of growth and the duration of the transient growth on the medium without growth regulators m the light decreased with the duration of the previous cultivation on the medium with growth regulators The intensity of growth on the medium without grow th regulators was best and the growth was permanent m the callus clone of spontaneous origin which was never treated by growth regulators The degree of chromosomal variability (assessed as the number of chromocentres) m this callus line was lower than that in calli induced on media with growth regulators and then transferred onto medium without growth regulatois.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4-dichlorphenoxyacetic acid
References
Bayer, M. H.: Genetic tumors: physiological aspects of tumor formation in interspecific hybrids. - In:Kahl, G., Schell, J. (ed.): Molecular Biology of Plant Tumors. Pp. 33–66. Academic Press, New York-London-Toronto-Sydney-San Francisco 1982.
Binns, A. N., Meins, J. R.: Chromosome number and the degree of cytokinin habituation of cultured tobacco pith cells. - Protoplasma103: 179–187, 1980.
Meins, F., Lutz, J.: The induction of cytokinin habituation in primary pith expiants of tobacco. - Planta149: 402–407, 1980.
Mouras, A., Lutz, A.: Dénobrement et analyse morphologique des chromosomes chez quelques clones issus d’une souche anergiée de tabac. Relation avec l’anergie, le pouvoir tumoral et l’organogenèse. - Genetica50: 195–199, 1979.
Negrutiu, I., Beeftink, F., Jacobs, M.:Arabidopsis thaliana as a new model system in somatic cell genetics I. Cell and tissue culture. - Plant Sci. Lett.5: 293–304, 1975.
Novak, F. J.: Chromosomal characteristics of long-term callus cultures ofAllium sativum L. - Cytobios49: 371–379, 1981.
Ondřej, M., Pavingerová, D., Našinec, V., Heoüda, M.: Growth requirements ofArabidopsis thaliana crown galls. - Biol. Plant.26: 5–10, 1984.
Otten, L., Schilperoort, R. A.: A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities. - Biochem. biophys. Acta527: 497–500, 1978.
Pavingerová, D., Ondřej, M., Matoušek, J.: Analysis of progeny ofArabidopsis thaliana plants regenerated from crown gall tumors. - Z. Pflanzenphysiol. (In press) 1984.
Röbbelen, G.: Untersuchungen an strahleninduzierten Blattfarbmutanten vonArabidopsis thaliana (L.). - Z. indukt. Abstammungs. Vererbungsl.88: 189–252, 1957.
Sacristan, M. D., Melchers, G.: Regeneration of plants from “habituated” and “Agrobacleriumtransformed” single-cell clones of tobacco. - Mol. gen. Genet.152: 111–117, 1977.
Sheridan, W. F.: Long-term lilly callus cultures. - In:Ledoux, L. (ed.): Genetic Manipulation with Plant Material. Pp. 263–295. Plenum Press, New York-London 1974.
Torrey, J. G.: Morphogenesis in relation to chromosomal constitution in long-term plant tissue cultures. - Physiol. Plant.20: 265–275, 1967.
Weber, E.: Grundriss der biologischen Statistik. - VEB Gustav Fischer. Jena 1964.
White, H. P., Braun, A. C.: A cancerous neoplasm in plants. Autonomous bacteria-free crown gall tissues. -Canc. Res.2: 597–617, 1942.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ondřej, M., Pešina, K. & Pavingerová, D. Hormonal autonomy ofArabidopsis thaliana calli. Biol Plant 26, 181–188 (1984). https://doi.org/10.1007/BF02895046
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02895046