Biologia Plantarum

, 23:228 | Cite as

Origin of cytokinin-and auxin-autonomy and changes in specific proteins in tobacco callus tissue

  • M. Kamínek
  • Věra Hadačová
  • J. Luštinec
Original Papers


On the basis of earlier data it was suggested that the induction of cytokinin autonomy might be accompanied by disorders in plastid function and a decrease in cytokinin utilization. In the work presented below the formation of chlorophyll and the isozyme patterns of nine enzymes, some of which are known to be localized in plastids, were compared in tobacco callus tissues differing in their hormonal requirements. Tissues either not requiring cytokinin or both auxin and cytokinin for their growth, contained a lower amount of chlorophyll than the cytokinin-and auxin-dependent strain. The number of isozymes of glucose-6-phosphate and NADP-malate dehydrogenase (i.e. enzymes which are known to be located in plastids) was reduced from four in the cytokinin-and auxin-dependent strain to two and one in the two cytokinin-autonomous strains, respectively. The fully habituated tissue contained an additional isozyme of NADP-malate dehydrogenase. The total number of isozymes of the remaining enzymes (NAD-malate dehydrogenase, peroxidase, esterase and a-and β-galactosidase) either was decreased or not changed in the cytokinin autonomous strains. The exception was an additional anodic peroxidase in one strain. The number of these isozymes in tissue habituated with respect to both auxin and cytokinin either remained the same or increased. Tobacco callus strains with altered requirements for growth regulators contained some new isozymes which were not present in any other strain and some isozymes present in other strains were absent. These differences are discussed in relation to the possible role of plastid function disorder associated with habituation.


Isozyme Pattern Disc Electrophoresis Tobacco Callus Culture Tobacco Cell Chlorophyll Formation 
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Abbreviations used


cytokinin- and auxin-dependent tobacco callus strain


fully habituated, i.e. cytokinin- and auxin-autonomous tobacco callus strain

As and A2,4-D

cytokinin-autonomous tobacco callus strains obtained after treatment of D tissue with streptomycin and 2,4-D, respectively


2,4-dichlorophenoxyacetic acid


malate dehydrogenase dependent on NAD and NADP, respectively


glucose-6-phosphate dehydrogenase


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Copyright information

© Academia 1981

Authors and Affiliations

  • M. Kamínek
    • 1
  • Věra Hadačová
    • 1
  • J. Luštinec
    • 1
  1. 1.Czechoslovak Academy of SciencesInstitute of Experimental BotanyPraha 6Czechoslovakia

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