Biologia Plantarum

, 27:432 | Cite as

Effect of gibberellic acid on organogenesis in buckwheat tissue culture

  • Veroslava Srejović
  • Mirjana Nešković


Buckwheat cotyledons were induced to form callus tissuein vitro, in which shoots or roots could be regenerated, by changing the hormonal composition of the medium. The effect of gibberellic acid (GA3) was studied in these processes. Addition of GA3 to cell division medium during the first 5 days after inoculation did not interfere either with the induction of cell division, or with the subsequent organogenesis. However, GA3 had a very pronounced effect on organogenesis when added immediately upon the transfer of expiants to organ induction medium. While root initiation was accelerated and root number increased, bud induction was inhibited. An initial GA3 treatment had a long-lasting effect on bud induction and the tissue recovered from inhibition only in the fifth subculture.


Gibberellin Gibberellic Acid Callus Tissue Jerusalem Artichoke Root Initiation 
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  1. Aghion-Prat, D.: Néoformation de fleursin vitro chezNicotiana tabacum L. - Physiol. vég.3: 229–303, 1965.Google Scholar
  2. Besemer, J., Harden, U., Reinert, J.: Der Einfluss von Kinetin und Gibberellinsäure auf die Organbildung anin vitro kultivierten Blättern vonCichorium intybus L. - Z. Pflanzenphysiol.60: 123–134, 1969.Google Scholar
  3. Bigot, C., Nitsch, J. P.: Effet du moment de l’application d’acide gibbérellique ou de 2,6-diaminopurine sur la néoformation des bourgeons chezBegonia rev Putz. - Compt. rend. Acad. Soi. Paris, Sér. D,267: 619–621. 1968.Google Scholar
  4. Coleman, W. K., Greyson, R. I.: Promotion of root initiation by gibberellie acid in leaf discs of tomato (Lycopersicon esculentum) culturedin vitro. - New Phytol.78: 47–54, 1977a.CrossRefGoogle Scholar
  5. Coleman, W. K., Greyson, R. I.: Analysis of root formation in leaf discs ofLycopersicon esculentum, Mill, culturedin vitro. - Ann. Bot.41: 307–320, 1977b.Google Scholar
  6. Gamborg, O. L., Miller, R. A., Otima, K.: Nutrient requirements of suspension cultures of soybean root cells. - Exp. Cell Res.50: 151–158, 1968.PubMedCrossRefGoogle Scholar
  7. Gautheret, R. G.: Investigations on the root formation in the tissues ofHelianthus tuberosus culturedin vitro. -Amer. J. Bot.56: 702–717, 1969.CrossRefGoogle Scholar
  8. Hasdon, L., Northoote, D. H.: The influence of gibberellic acid and abscisic acid on cell and tissue differentiation of bean callus. - J. Cell Sci.20: 47–55, 1976.Google Scholar
  9. Hansen, J.: Light dependent promotion and inhibition of adventitious root formation by gibberellic acid. - Planta123: 203–205, 1975.CrossRefGoogle Scholar
  10. Heide, O. M.: Non-reversibility of gibberellin-induced inhibition of regeneration inBegonia leaves. - Physiol. Plant.22: 671–679, 1969.CrossRefGoogle Scholar
  11. Margara, J.: Effets d’auxines et d’antiauxines sur la néoformation de bourgeonsin vitro chez le chou-fleur (Brassica oleracea L., var.botrytis). - Compt. rend. Acad. Sci., Paris. Sér. D284: 1883–1885, 1977.Google Scholar
  12. Murashige, T.: Analysis of the inhibition of organ formation in tobacco tissue culture by gibberellin. - Physiol. Plant.17: 636–653, 1964.CrossRefGoogle Scholar
  13. Rücker, W.: Kombinierter Einfluss von Indolessigsäure, Gibberellin und Benzylaminopurin auf Kallus- und Organdifferenzierung an Blattexplantaten vonDigitalis purpurea. - Z. Pflanzenphysiol.107: 141–151, 1982.Google Scholar
  14. Skoog, F., Miller, C. O.: Chemical regulation of growth and organ formation in plant tissues culturedin vitro. - Symp. Soc. exp. Biol.11: 118–131, 1957.Google Scholar
  15. Smith, D. B., Thorpe, T. A.: Root initiation in cuttings ofPinus radiata seedlings. II. Growth regulator interactions. - J. exp. Bot.26: 193–202, 1975.CrossRefGoogle Scholar
  16. Srejović, V., Nešković, M.: The presence of gibberellin-like substances in germinating embryos of different plant species. - Arh. biol. Nauka (Beograd)25: 107–117, 1973.Google Scholar
  17. Seejović, V., Nešković, M.: Regeneration of plants from cotyledon fragments of buckwheat (Fagopyrum esculentum Moench.). - Z. Pflanzenphysiol.104: 37–42, 1981.Google Scholar
  18. Tanimoto, S., Habada, H.: Chemical factors controlling floral bud formation ofTorenia stem segments culturedin vitro. II. Effects of growth regulators. - Plant Cell Physiol.22: 543 to 550, 1981.Google Scholar
  19. Thorpe, T. A.: Organogenesisin vitro: Structural, physiological, and biochemical aspects. - In:Vasil, I. K. (ed.): Perspectives in Plant Cell and Tissue Culture. (Int. Eev. Cytol. Suppl. 11A.) Pp. 71–111. Academic Press, New York 1980.Google Scholar
  20. Thorpe, T. A., Meier, D. D.: Effects of gibberellic acid and abscisic acid on shoot formation in tobacco callus cultures. - Physiol. Plant.29: 121–124, 1973.CrossRefGoogle Scholar
  21. Webb, D. T., Torres, L. D.: Interactions of growth regulators, expiant age, and culture environment controlling organogenesis from lettuce cotyledonsin vitro. - Can. J. Bot.62: 586–590, 1984.CrossRefGoogle Scholar

Copyright information

© Academia 1985

Authors and Affiliations

  • Veroslava Srejović
    • 1
  • Mirjana Nešković
    • 2
  1. 1.Faculty of ScienceInstitute of BiologyKragujevac
  2. 2.Faculty of ScienceInstitute of BotanyBeogradYugoslavia

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