Abstract
Occurrence and operation of sucrose synthetic system in randomly selected callus cultures such as persimmon, soybean and poplar cultures were examined by14C-tracer analysis and determining enzyme activities involved in sucrose metabolism. All the enzymes examined were present and14C-glueose was transformed into14C-sucrose in every callus. Sucrose synthetic capacity appears to be widely distributed in cultured plant tissues.
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Goris, A.: Transformations glucidiques intratissulaires. — Anné. Biol.30: 297–318, 1954.
Hisajima, S.: The conversion of external sugars to internal sucrose in morning-glory callus. — Radioisotopes24: 403–409, 1975.
Hisajima, S.: Changes in sugar contents and some enzyme activities during the growth of Japanese morning-glory callus. — J. jap. Soc. Starch Sci.25: 223–228, 1978.
Hisajima, S.: Studies on the sucrose metabolism in callus cells of Japanese morning-glory. — Memoirs Tokyo Univ. Agr.21: 1–55, 1979.
Hisajima, S., Arai, Y.: Cell wall-bound invertase of morning-glory callus. — J. jap. Soc. Starch Sci.25: 164–170, 1978.
Hisajima, S., Hasegawa, T., Ito, T., Suzuki, T.: Partial purification and properties of sucrose phosphate synthase from morning-glory callus cells. — J. jap. Soc. Starch Sci.27: 167–172, 1980.
Maretzki, A., Thom, M., Nickell, L. G.: Utilization and metabolism of carbohydrates in cell and tissue culture. — In:Street, H. E. (ed.): Tissue Culture and Plant Science. Pp. 329–361. Academic Press, New York 1974.
Thorpe, T. A.: Carbohydrate utilization and metabolism. — In:Bonga, J. M., Durzan, D. J. (ed.): Tissue Culture in Forestry. Pp. 325–368. Martinus Nijhoff/Dr. W. Junk, The Hague 1982.
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Hisajima, S., Arai, Y. & Thorpe, T.A. Sucrose synthesis in callus cultures. Biol Plant 27, 74–77 (1985). https://doi.org/10.1007/BF02894639
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DOI: https://doi.org/10.1007/BF02894639