Biologia Plantarum

, 28:230 | Cite as

The uptake of3H-thymidine and its transport inDatura stramonium L.

  • Marie Sovová
  • V. Šeda
  • Věra Spurná
Original Papers


Exogenous3H-thymidine is absorbed by the primary root of young plants ofDatura stramonium L. and gradually translocated into shoots: following a 3 to 72-h application of3H-thymidine the radioactivity was revealed, using the autoradiographic technique, especially in the region of primary and secondary meristems and in proximity to vascular bundles of the primary root, stem, hypocotyl and leaves. These regions may be considered as the sites of active DNA synthesis. The intensity of incorporation was dependent on the time of plant incubation in labelled thymidine.


Thymidine Vascular Bundle Shoot Apex Primary Root Tritiated Thymidine 


  1. Brunori, A.: Synthesis of DNA and mitosis in relation to cell differentiation in the roots ofVicia faba andLactuca sativa. - Caryologia24: 209–215, 1971.Google Scholar
  2. Hurst, P. R., Gahan, P. B.: Turnover of DNA in ageing tissues ofLycopersicon esculentum. - Ann. Bot.39: 71–76, 1975.Google Scholar
  3. Hurst, P. R., Gahan, P. B., Snellen, J. W.: Turnover of labelled DNA in differentiated collenchyma. - Differentiation1: 261–264, 1973.CrossRefGoogle Scholar
  4. Jalouzot, R., Lechenault, H., Gontcharoff, M.: Etude autoradiographique et cytophotométrique de la phase la plus précoce de l’activation cellulaire au cours de l’initiation de racines adventices chez la fève (Vicia faba). - Compt. rend. Acad. Sci. (Paris) Sér. D280: 1733–1736, 1975.Google Scholar
  5. MacLeod, R. D.: Total uptake and incorporation into DNA of3-thymidine,3H-deoxyuridine and3H-thymine in the primary root ofVicia faba L. - J. exp. Bot.23: 899–906, 1972. 233CrossRefGoogle Scholar
  6. MacLeod, R. D.:3H-thymidine utilization in the primary root ofVicia faba L. - New Phytol.72: 259–267, 1973.CrossRefGoogle Scholar
  7. MacLeod, R. D., McLachlan, S. M.: Triated-thymidine labelled nuclei in primordia and newly-emerged lateral roots ofVicia faba L. - Ann. Bot.39: 535–545, 1975.Google Scholar
  8. Maksymowych, R., Kettrick, M. A.: DNA synthesis, cell division, and cell proliferation during leaf development ofXanthium pennsylvanicum. - Amer. J. Bot.57: 844–849, 1970.CrossRefGoogle Scholar
  9. Maksymowych, R., Maksymowych, L. H.: Incorporation of triated thymidine during various stages of leaf development ofXanthium pennsylvanicum. - Amer. J. Bot.60: 529–534, 1973.CrossRefGoogle Scholar
  10. Pelc, S. R.: Metabolic DNA in ciliated Protozoa salivary gland chromosomes and mammalian cells. – Int. Rev. Cytol.32: 327–355, 1972.PubMedCrossRefGoogle Scholar
  11. Pelc, S. R., LaCour, L. F.: The incorporation of3H-thymidine in newly differentiated nuclei of roots ofVicia faba L. - Experientia15: 131–133, 1959.PubMedCrossRefGoogle Scholar
  12. Sallé, G.: Nuclear DNA metabolism in the tip of the cortical strands ofViscum album L. - Ann. Bot.43: 1171–1176, 1978.Google Scholar
  13. Scadeng, D. W. F., MacLeod, R. D.: The effect of sucrose concentration on cell proliferation and quiescence in the apical meristem of excised roots ofPisum sativum L. - Ann. Bot.40: 947–955, 1976.Google Scholar
  14. Yadav, S. P.: Rhythmic increase in DNA content and incorporation of3H thymidine in the developing wheat embryo during seed germination. - Planta129: 87–89, 1976.CrossRefGoogle Scholar

Copyright information

© Academia 1986

Authors and Affiliations

  • Marie Sovová
    • 1
  • V. Šeda
    • 1
  • Věra Spurná
    • 2
  1. 1.Faculty of Pharmacy, Department of BotanyCharles UniversityHradec KrálovéCzechoslovakia
  2. 2.Institute of BiophysicsCzechoslovak Academy of SciencesBrnoCzechoslovakia

Personalised recommendations