Stability of total and free prostate specific antigen in serum samples at different storage conditions
The present preliminary study was performed to find out stability of total prostate specific antigen (PSA) and free prostate specific antigen (FPSA) in serum of healthy males as well as in patients of benign and malignant disorders of prostate at various freezing and nonfreezing temperatures and at different duration of time.
The results of our study indicated long-term stability of both the analytes in frozen serum. Serum total and free PSA were stable only for three to four days in regular refrigerators in unfrozen states. Clotted blood kept at room temperature (25°C–30°C) did not cause change in concentrations of both the analytes for twenty four hours.
Key WordsFree PSA and Total PSA BPH Cancer Prostate
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- 1.Pettersson, K., Piironen, T., Seppala, M., Liukkonen, L., Christensson, A., Matikainen, M.T., Suonpaa, M., Lovgren, T. and Lilja, H. (1995). Free and complexed prostate-specific antigen (PSA):in vitro stability, epitope map, and development of immunofluorometric assays for specific and sensitive detection of free PSA and PSA-∞1-antichymotrypsin complex. Clin. Chem. 41, 1480–1488.PubMedGoogle Scholar
- 5.B.M. multicentre international study PSA free (1996) Boerhinger Mannheim Lab Diagnostics, Mannheim, Germany.Google Scholar
- 7.Piironen, T., Pettersson, K., Suonapaa, M., Stenman, U.H., Oesterling, J.E., Lovgren, T. and Lilja, H. (1996)In vitro stability of free prostate-specific antigen (PSA) and prostate-specific antigen (PSA) complexed to ∞1-antichymotrypsin in blood samples. Urology. 48 (6A), 81–87.PubMedCrossRefGoogle Scholar