Genetic study of kelp “901” strain
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Based on DNA extraction and optimization of random amplified reaction (RAPD) to the gametophytes and sporophytes of Kelp “901” strain, genetic study on variation was conducted to its parents and offsprings of F6, F7, F8, and F9 generation. RAPD results have shown that among 30 selected primers for gametophytes, 297 loci ranging from 200 to 3 000 bp were obtained in the average of 9.9 loci for each primer. This indicated a high polymorphic rate with RAPD detection. UPGMA (unweighted pair-group method arithmetic average) analysis showed that each male and female gametophyte of a generation could be clustered into one pair separately. The genetic distances of the Kelp 901 generation were 0.3212–0.4767, and the maximum was between F7 and F8 (0.4767). Identity analysis showed that F6 generation was more close to the female parent (0.6593), and F7 generation was more close to the male parent (0.578 8). To the sporophytes study in 24 selected primers for RAPD amplification, 191 loci ranging from 230–2800 bp were obtained, in the average to each primer of 8.0 loci. The heterozygosity to six populations were male parent (0.2239), female parent (0.1072), F6 (0.2164), F7(0.2286), F8(0.2296) and F9(0.3172). The nearest genetic distance was 0.083 5(F8, F9). Total heterozygosity (HT) of F6, F7, F8 and F9 generations was 0.3186, the average heterozygosity (HS) for F6, F7, F8 and F9 generations was 0.2480, and deduced coefficient of population differentiation (Gst) was 22.2%. Six sequence characterized amplified regions (SCAR) were preliminary screened through RAPD analysis. It needed to be verified in detail as they are significant for molecular marker assistance in breeding and selectingLaminaria.
Key wordsLaminaria “901” strain gametophyte sporophyte RAPD genetics variation
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- Bolton, J. J., I. Germann and K. Luning, 1983. Hybridization between Altanic and Pacific representatives of the simplices section ofLaminaria (Phaeophyta).Phycologia 22(2): 131–140.Google Scholar
- Dudley, J. W., M. A. Saghai and G. K. Rufener, 1991. Molecular markers and grouping of parents in maize breeding programs.Crop Science 31: 718–723.Google Scholar
- Fang, T. C., C. Y. Wu, B. Y. Jiang, J. J. Li, and K. Z. Ren, 1962. The breeding of a new breed of Haidai (Laminaria japonica Aresch.) and its preliminary genetic analysis.Acta Botanica Sinica 10(3): 197–209. (in Chinese with English abstract)Google Scholar
- Fang, T. C., B. Y. Jiang and J. J. Li, 1965. Further studies of the genetics ofLaminaria frond-length.Oceanologia et Limnologia Sinica 7(1): 59–66. (in Chinese with English abstract)Google Scholar
- Fang, T. C., B. Y. Jiang and J. J. Li, 1962. On the inheritance of stipe length in Haidai (Laminaria japonica Aresch.).Acta Botanica Sinica 10(4): 327–335. (in Chinese with English abstract)Google Scholar
- Fain, S. R., L. D. Druehl and D. L. Baillie, 1988. Repeat and single copy sequences are differentially conserved in the evolution of kelp chloroplast DNA.J. Phycol 24: 292–302.Google Scholar
- He, Y. J., Y. P. Zou, X. D. Wang, Z. G. Zheng, D. M. Zhang and D. L. Duan, 2003. Assessing the germplasm ofLaminaria (Phaeophyceae) with random amplified polymorphic DNA (RAPD) method.Chin. J. Oceanol. Limnol. 21(3): 141–148.Google Scholar
- Luning, K., R. O. Chapman and K. H. Mann, 1978. Cross experiments in the non-digitate complex ofLaminaria from both sides of the Atlantic.Phycologia 17(3): 293–298.Google Scholar
- Tseng, C. K., 1986.Laminaria mariculture in China.In: Doty, M. S., Caddy, J. F. eds., Santelices case studies of seven commercial seaweed resources. FAO Fisheries technical paper. p. 281, 239–263.Google Scholar
- Zhang, Q. S., S. P. Liu, S. C. Qu, Z. P. Tian, Z. M. Guo, Y. Z. Cong and Z. L. Li, 2001. Studies on rearing new variety ofKelp “901”.Transactions of Oceanology and Limnology 2: 46–53. (in Chinese with English abstract)Google Scholar
- Zhang, J. Y. and Z. X. Fang, 1980. Preliminary observations on the inheritance of frond thickness inLaminaria japonica.Acta Genetica Sinica 7(3): 257–262. (in Chinese with English abstract)Google Scholar