Virchows Archiv B

, 9:280 | Cite as

Nuclear proliferation and the amount of DNA per parenchymal nucleus during mouse liver regeneration

  • L. Lewan


When the number of parenchymal nuclei in regenerating mouse liver was estimated on the basis of nuclear counts in vestopal embedded sections, higher values were obtained than when the estimation was made on the basis of nuclear counts in homogenates. A selective fragmentation of small, newly formed nuclei is indicated, which may have contributed to an overestimation of the increasing polyploidy during mouse liver regeneration in earlier investigations. Studies on homogenates revealed that the stability of the cell membrane increased during the regeneration. This may have made it more difficult to obtain parenchymal cell nuclei in an unbroken state, free from cells, at homogenization.

The increase in the number of nuclei estimated in liver sections, during a period of 140 hours after partial hepatectomy, is in accordance with that indicated by the mitotic index, assuming that the mean mitotic duration is between one and two hours. Values in this range have actually been found in some preliminary experiments with colchicine. An initially high increase in the amount of DNA per nucleus indicates a long duration of the S and G2 phases or possibly an accumulation in the liver of chromatin products from degraded tissues.


Colchicine Mouse Liver Mitotic Index Liver Regeneration Partial Hepatectomy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Abercrombie, M., Harkness, R. D.: The growth of cell populations and the properties in tissue culture of regenerating liver of the rat. Proc. roy. Soc. B138, 544–561 (1951).CrossRefGoogle Scholar
  2. Aronsen, K. F., Nordén, J. G., Nylander, G., Ohlsson, E. G., Pihl, B.: On the vascular anatomy of the liver following partial hepatectomy in the dog. Acta chir. scand.136, 413–418 (1970).PubMedGoogle Scholar
  3. Ballard, P. L., Tomkins, G. M.: Hormone induced modification of the cell surface. Nature (Lond.)224, 344–345 (1969).CrossRefGoogle Scholar
  4. Barnum, C. P., Jardetzky, C. D., Halberg, F.: Nucleic acid synthesis in regenerating liver. Tex. Rep. Biol. Med.15, 134–147 (1957).PubMedGoogle Scholar
  5. Bengmark, S.: Liver regeneration. In: Tumours of the liver, ed. by Pack, G. T. and Islami, A. H., p. 187–212. Berlin-Heidelberg-New York: Springer 1970.Google Scholar
  6. — Olsson, R., Svanborg, A.: Fat infiltration after partial hepatectomy. Changes in glycerides, cholesterol and phospholipids in the residual liver of the rat. Acta hepato-splenol. (Stuttg.)11, 276–285 (1970).Google Scholar
  7. Blobel, G., Potter, V. R.: Nuclei from rat liver: Isolation method that combines purity with high yield. Science154, 1662–1665 (1966).PubMedCrossRefGoogle Scholar
  8. Brown, J. M., Berry, R. J.: The relationship between diurnal variation of the number of cells in mitosis and of the number of cells synthesizing DNA in the epithelium of the hamster cheek pouch. Cell Tiss. Kinet.1, 23–33 (1968).Google Scholar
  9. Brues, A. M. Drury D. R., Brues, M. C.: A quantitative study of cell growth in regenerating liver. Arch. Path.22, 658–673 (1936).Google Scholar
  10. Bryant, B. J.: Reutilization of lymphocyte DNA by cells of intestinal crypts and regenerating liver. J. Cell Biol.18, 515–523 (1963).PubMedCrossRefGoogle Scholar
  11. Bucher, N. L. R.: Regeneration of mammalian liver. Int. Rev. Cytol.15, 245–300 (1963).PubMedGoogle Scholar
  12. — Experimental aspects of hepatic regeneration. New Engl. J. Med.277, 686–696, 738–746 (1967).PubMedGoogle Scholar
  13. — Glinos, A. D.: The effect of age on regeneration of rat liver. Cancer Res.10, 324–332 (1950).PubMedGoogle Scholar
  14. Bucher, O., Suppan, P.: Amitose et fusion nucléaires au cours du rythme circadien. In: The cellular aspects of biorhythms, ed. by Mayersbach, H., p. 124–132. Berlin-Heidelberg-New York: Springer 1967.Google Scholar
  15. Bullough, W. S.: The control of mitotic activity in adult mammalian tissues. Biol. Rev.37, 307–342 (1962).PubMedCrossRefGoogle Scholar
  16. — Laurence, B.: The role of glucocorticoid hormones in the control of epidermal mitosis. Cell Tiss. Kinet.1, 5–10 (1968).Google Scholar
  17. Daoust, R.: The cell population of liver tissue and the cytological refence bases. In: Liver function, ed. by Brauer, R. W., p. 3–10. Washington: Am. Inst. Biol. Science 1958.Google Scholar
  18. Dounce, A. L.: The isolation and composition of cell nuclei and nucleoli. In: The nucleic acids, vol. 2, ed. by Chargaff, E., Davidsson, J. N., p. 93–154. New York: Academic Press, Inc. 1955.Google Scholar
  19. Echave Llanos, J. M., D’Aloisso, M., Souto, M., Balduzzi, R., Surur, J. M.: Circadian variations of DNA synthesis, mitotic activity and cell size of hepatocyte population in young immature male mouse growing liver. Virchows Arch. Abt. B.8, 309–317 (1971).Google Scholar
  20. — Bade, E. G., Badrian, A. F.: Circadian rythms in growth processes. In: The cellular aspects of biorythms, ed. by Mayersbach, H., p. 175–180. Berlin-Heidelberg-New York: Springer 1967.Google Scholar
  21. — Bordin, C.: The growth effect of a regenerating liver homogenate as a function of the responsiveness of the receptor. Naturwissenschaften50, 501 (1963).CrossRefGoogle Scholar
  22. — Gomez Dumm, C. L., Nessi, A. C.: Ultrastructure of STH Cells of the pars distalis of hepatectomized mice. Z. Zeilforsch.113, 29–38 (1971).CrossRefGoogle Scholar
  23. Edwards, J. L., Koch, A. K.: Parenchymal and littoral cell proliferation during liver regeneration. Lab. Invest.13, 32–43 (1964).PubMedGoogle Scholar
  24. Fabrikant, J. I.: The effect of prior continuous irradiation on the G2, M and S phases of proliferating parenchymal cells in the regenerating liver. Radiat. Res.31, 304–314 (1967).PubMedCrossRefGoogle Scholar
  25. — The kinetics of cellular proliferation in regenerating liver. J. Cell Biol.36, 551–565 (1968).PubMedCrossRefGoogle Scholar
  26. Grisham, J. W.: A morphological study of deoxyribonucleic acid synthesis and cellproliferation in regenerating rat liver; Autoradiogaraphy with thymidine-H3. Cancer Res.22, 842–849 (1962).PubMedGoogle Scholar
  27. Grundman, E., Bach, G.: Amitosen, Endomitosen und Mitosen nach partieller Hepatektomie. Beitr. path. Anat.123, 144–172 (1960).Google Scholar
  28. — Seidel, H. J.: Die reparative Parenchymregeneration am Beispiel der Leber nach Teilhepatektomie. In: Handbuch der allgemeinen Pathologie, VI/2, hrsg. von Büchner, F., p. 129–243. Berlin-Heidelberg-New York: Springer 1969.Google Scholar
  29. Harkness, R. D.: Regeneration of liver. Brit. med. Bull.13, 87–93 (1957).PubMedGoogle Scholar
  30. Heby, O., Lewan, L.: Putrescine and polyamines in relation to nucleic acids in mouse liver after partial hepatectomy. Virchows Arch. Abt. B8, 58–66 (1971).Google Scholar
  31. Jaffe, J. J.: Diurnal mitotic periodicity in regenerating rat liver. Anat. Rec.120, 935–954 (1954).PubMedCrossRefGoogle Scholar
  32. James, J., Schopman, M., Delfgaauw, P.: The nuclear pattern of the parenchymal cells of the liver after partial hepatectomy. Exp. Cell Res.42, 375–379 (1966).PubMedCrossRefGoogle Scholar
  33. Johnston, I. R., Mathias, A. P., Pennington, F., Ridge, D.: The fractionation of nuclei from mammalian cells by zonal centrifugation. Biochem. J.109, 127–135 (1968).PubMedGoogle Scholar
  34. Lancker, J. L. van, Sempoux, D. G.: Regeneration of various cellular components in rat livers after subtotal hepatectomy. Arch. Biochem.77, 129–137 (1958).CrossRefGoogle Scholar
  35. Landing, B. H., Seed, J. C., Banfield, W. G.: The effect of a nitrogen mustard on regenerating rat liver. Cancer (Philad.)2, 1067–1074 (1949).CrossRefGoogle Scholar
  36. Nadal, C., Zaidela, F.: Polyploidie somatique dans le foie de rat. Exp. Cell Res.42, 99–116 (1966).PubMedCrossRefGoogle Scholar
  37. Millonig, F.: Further observations on a phosphate buffer for osmium solution in fixation. In: 5th International Conference on Electron Microscopy, vol. II, p. 8. New York: Academic Press Inc. 1962.Google Scholar
  38. Orlova, L. V., Rodionov, V. M.: The time of histone synthesis in regenerating rat liver. Exp. Cell Res.59, 329–333 (1970).PubMedCrossRefGoogle Scholar
  39. Price, J. M., Laird, A. K.: A comparison of the intracellular composition of regenerating liver and induced liver tumors. Cancer Res.10, 650–658 (1950).Google Scholar
  40. Rabes, H., Hartenstein, R., Scholze, P.: Specific stages of cellular response to homeostatic control during diethylnitrosamine induced liver carcinogenesis. Exper.26, 1356–1359 (1970).CrossRefGoogle Scholar
  41. — Tuczek, H. V.: Quantitative autoradiographische Untersuchung zur Heterogenität der Leberzellproliferation nach partieller Hepatektomie. Virchows Arch. Abt. B6, 302–312 (1970).Google Scholar
  42. Rüdeberg, C.: A rapid method for staining thin sections of vestopal W-embedded tissue for light microscopy. Experientia (Basel)23, 792 (1967).Google Scholar
  43. Tannock, I. F.: A comparison of the relative efficiencies of various metaphase arrest agents. Exp. Cell Res.47, 345–356 (1967).CrossRefGoogle Scholar
  44. Trotter, N. L.: A fine structure study of lipid in mouse liver regenerating after partial hepatectomy. J. Cell Biol.21, 233–244 (1964).PubMedCrossRefGoogle Scholar
  45. Tsuboi, K. K., Yokoyama, H. O., Stowell, R. E., Wilson, M. E.: The chemical composition of regenerating mouse liver. Arch. Biochem.48, 275–292 (1954).PubMedCrossRefGoogle Scholar
  46. Tygstrup, N.: Levercellens reaktion på beskadigelse. In: Medic. Årsbok Bd. X, ed. by Husted, E. p. 216–228. Köpenhamn: Munksgaards förl. 1967.Google Scholar
  47. Wilson, W. J., Leduc, H. L.: The occurence and formation of binucleate and multinucleate cells and polyploid nuclei in the mouse liver. Amer. J. Anat.82, 353–391 (1948).CrossRefPubMedGoogle Scholar
  48. Wilson, M. E., Stowell, R. E., Yokoyama, O., Tsuboi, K. K.: Cytological changes in regenerating mouse liver. Cancer Res.13, 86–92 (1953).PubMedGoogle Scholar
  49. Yokoyama, H. O., Wilson, M. E., Tsuboi, K. K., Stowell, R. E.: Regeneration of mouse liver after partial hepatectomy. Cancer Res.13, 80–85 (1953).PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • L. Lewan
    • 1
  1. 1.Institute of ZoophysiologyUniversity of LundLundSweden

Personalised recommendations