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Virchows Archiv B

, Volume 12, Issue 1, pp 259–272 | Cite as

Fine structure of germinal centers of the splenic lymphatic tissue of the mouse, with special reference to the occurrence of peculiar intercellular globules in the light zone

  • K. Abe
  • T. Ito
Article
  • 34 Downloads

Summary

The fine structure of germinal centers of the spleen, mesenteric lymph node and Peyer’s patch of the adult mouse was observed with the light and electron microscope.

The mature germinal centers of the spleen consisted of two hemispherical zones, light and dark. The different staining of the two zones was due largely to differences in cellular population. Ultrastructurally the light zone was characterized by a prominent elaboration of complex labyrinthine intercellular spaces that were bounded by numerous microvillous cytoplasmic processes of “dendritic” reticular cells and lymphoid cells. The intercellular spaces contained numerous peculiar globules, in addition to amorphous electron-opaque material. The globules varied in diameter from 200 to 7000 Å, and generally showed high electron density. Parallel examination in Sudan-stained sections revealed that the globules contained lipid. Such globules were scarcely seen in germinal centers of the mesenteric lymph node and Peyer’s patches.

After intraperitoneal injection of typhoid-paratyphoid vaccine the globules in the light zone of the spleen disappeared transiently, but appeared again at 7 days. After the second injection of the same antigen, however, few or almost no globules reappeared even at 7 days. At the same time the splenic germinal centers were enlarged and the intercellular spaces in the light zone were expanded more widely.

The splenic germinal centers after secondary immunization were similar in structure to germinal centers of the mesenteric lymph node and Peyer’s patches.

The findings on the globules in the light zone of the splenic germinal centers were discussed in relation to the immune response.

Keywords

Lymphoid Cell Intercellular Space Germinal Center Mesenteric Lymph Node Reticular Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • K. Abe
    • 1
  • T. Ito
    • 1
  1. 1.Department of AnatomyHokkaido University School of MedicineSapporoJapan

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