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Pathology & Oncology Research

, Volume 2, Issue 1–2, pp 21–29 | Cite as

Tyrosine phosphorylation of a ∼30 kd protein precedes αvβ3 integrin-signaled endothelial cell spreading and motility on matrix proteins

  • Dean G. Tang
  • Clement A Diglio
  • Kenneth V Honn
Article
  • 3 Downloads

Abstract

A microvascular endothelial cell line (CD clone 4) isolated from murine lung adheres to and spreads well on fibronectin, vitronectin, and fibrinogen, but poorly on collagen type IV and laminin. Ligating cell surface αv, β3, α4, α5, or β1 integrin receptors with monospecific antibodies promoted a dramatic cell spreading and motility on vitronectin or collagen IV. Antibodies directed to other adhesion molecules, including αIIb, PECAM-1, and P-selectin were ineffective. Ligation with monoclonal anti-αv or -β3, but not -α4, -α5, or -β1 antibodies, induced a rapid, and dose-dependent tyrosine phosphorylation of a ∼30 kD protein, which preceded CD clone 4 endothelial cell spreading and motility and was partially inhibited by genistein and completely inhibited by BAPTA. All other antibodies tested did not induce the tyrosine phosphorylation of the 30 kD protein as well as cell spreading and motility. The present results suggest that β1 and β3 integrins employ different biochemical mechanisms in signaling endothelial cell spreading and motility and that the tyrosine phosphorylation of the 30 kD protein (and probably other proteins) may play an important role in signaling β3 integrin-mediated endothelial cell interaction with other cells (e.g., tumor cells) and extracellular matrix.

Key words

integrin motility spreading tyrosin phosphorylation extracellular matrix 

Abbreviations

Ab

antibody

BAPTA-AM

bis(2-aminophenoxy)-ethane-N, N, N N’-tetraacetate, acetoxymethyl ester

DMEM

Dulbecco’s Minimum Essential Medium

FAK

focal adhesion kinase

mAb

monoclonal antibody

pAb

polyclonal antibody

PKC

protein kinase C

SKM

subendothelial matrix

Vn

vitronectin

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Copyright information

© Arányi Lajos Foundation 1996

Authors and Affiliations

  • Dean G. Tang
    • 1
  • Clement A Diglio
    • 2
  • Kenneth V Honn
    • 1
    • 3
  1. 1.Department of Radiation OncologyWayne State UniversityDetroitUSA
  2. 2.Department of Radiation PathologyGershenson Radiation Oncology CenterDetroit
  3. 3.Department of Radiation ChemistryHarper HospitalDetroitUSA

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