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Virchows Archiv B Cell Pathology Zell-pathologie

, Volume 1, Issue 1, pp 230–239 | Cite as

Riesenmitochondrien aus Onkocyten menschlicher Adenolymphome

Isolierung, morphologische und biochemische Untersuchungen
  • H. -G. Schiefer
  • G. Hübner
  • O. Kleinsasser
Article
  • 22 Downloads

Zusammenfassung

Aus Adenolymphomen der Gl. parotis wurden die Riesenmitochondrien der in diesen Geschwülsten vorkommenden Onkocyten isoliert. Cytochromspektren und Lipoidanalyse zeigen keine wesentlichen Abweichungen von den Mitochondrien anderer Organe und Species. Obwohl die Onkocytenmitochondrien normalerweise keine Ca++-haltigen strahlendichten Matrixeinschlüsse besitzen, nehmen sie wie normale Mitochondrien in Ca++-haltigem Medium reichlich Calcium auf. Elementarpartikel können gelegentlich an den Membranen der Mitochondrien nachgewiesen werden. An den isolierten Onkocytenmitochondrien wurden niedrige P:O-Quotienten und eine geringe Kopplung von Atmung und Phosphorylierung gemessen. Dieser für die Ursache der onkocytären Mitochondriopathie wichtige Befund bedarf weiterer Klärung.

Giant mitochondria from oncocytes of human adenolymphomas of the parotid gland

Isolation, morphological and biochemical investigations

Summary

Giant mitochondria were isolated from oncocytes of human adenolymphomas. The analysis of cytochrome spectra and lipids did not show any defect or deviation. Elementary particles can be demonstrated after negative staining. In vivo, the giant mitochondria from oncocytes do not show electron-dense granules in their matrix; in vitro, however, they show a massive Ca++-loading when incubated under conditions favorable for maximum accumulation of Ca++ and Pi. Preliminary experiments show lowered P:O-ratios, a loosely coupled state of oxidative phosphorylation and a low sensitivity to oligomycin.

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Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • H. -G. Schiefer
    • 1
  • G. Hübner
    • 2
  • O. Kleinsasser
    • 3
  1. 1.Physiologisch-Chemisches Institut der Universität zu KölnDeutschland
  2. 2.Pathologisches Institut der Universität zu KölnDeutschland
  3. 3.Hals-Nasen-Ohren-Klinik der Universität zu KölnDeutschland

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