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Pathology & Oncology Research

, Volume 11, Issue 2, pp 92–97 | Cite as

Tumor-infiltrating B cell immunoglobulin variable region gene usage in invasive ductal breast carcinoma

  • Peter Simsa
  • Jean-Luc Teillaud
  • David I. Stott
  • József Tóth
  • Beatrix Kotlan
Article

Abstract

A major focus of tumor immunology is to reveal the potential role and capacity of immunocompetent cells found in different solid tumor tissues. The most abundant infiltrating cells (TIL), the T lymphocytes have been investigated in details concerning T-cell receptor usage and specificity. However, B cells have hardly been investigated in this respect, although high cellular B-cell infiltration has been correlated with improved patients’ survival in some breast carcinomas. This led to our objectives to study variable region gene usage of the tumor-infiltrating B cells in different breast carcinoma types. By defining the immunoglobulin repertoire of the tumor-infiltrating B lymphocytes in the most common invasive ductal carcinoma (IDC) of the breast we compared it to the rare medullary breast carcinoma (MBC). After phenotyping infiltrating ductal carcinomas, B cells were obtained from tumor tissue by microdissection technique. Numerous rearranged TIL-B immunoglobulin heavy chain V genes (VH) were amplified, cloned, sequenced, and comparatively analyzed. Some characteristics were found for both breast carcinoma types. The immunoglobulins produced by TIL-B in ductal carcinoma are highly matured and oligoclonal. We conclude that Ig variable region gene usage reveals similar and distinguishable characteristics of TIL-B immunoglobulin repertoires, which are representative of the nature of the immune responses in invasive ductal and medullary breast carcinomas.

Key words

immunoglobulin variable region breast ductal carcinoma tumor-infiltrating lymphocytes 

Abbreviations

Aa

amino acid

CDR

complementary determining region

DCIS

ductal carcinoma in situ

FDC

follicular dendritic cell

FR

framework region

IDC

invasive ductal carcinoma

Ig

immunoglobulin

MBC

medullary breast carcinoma

PBMC

peripheral blood mononuclear cell

TIL-T

tumor-infiltrating T lymphocytes

TIL-B

tumor-infiltrating B lymphocytes

VH

Ig heavy chain variable region

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Copyright information

© Arányi Lajos Foundation 2005

Authors and Affiliations

  • Peter Simsa
    • 1
  • Jean-Luc Teillaud
    • 2
  • David I. Stott
    • 3
  • József Tóth
    • 4
  • Beatrix Kotlan
    • 1
  1. 1.National Medical CenterInstitute of Haematology and ImmunologyBudapestHungary
  2. 2.INSERM U255Centre de Recherches Biomedicales des CordeliersParisFrance
  3. 3.Division of Immunology, Infection and InflammationUniversity of Glasgow, Western InfirmaryGlasgowUK
  4. 4.National Institute of OncologyBudapestHungary

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