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Comparative effect of olive oil and fish oil supplementation in combating gentamicin induced nephrotoxicity in rats

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Abstract

The present study is related to the comparative effects of fish oil and olive oil supplementation on gentamicin induced nephrotoxicity in rats. Three treatment groups (Pretrement, Co-treatment and post treatment) were chosen for the study. Nephrotoxicity in rats was induced by intraperitonial administration of gentamicin (80 mg/kg/d) for 3,5,7,10,& 12 consecutive days. The animals were sacrificed 12 hrs after last treatment in each group. The maximum nephrotoxicity was developed on 10 days treatment of gentamicin. For each group a control group was taken without any oil or gentamicin treatment. Beneficial effects of oils were evidenced by reduced serum urea and creatinine concentrations in the group receiving oils compared to the non oil treatment animals receiving gentamicin only. Further, the changed values of alkaline phosphatase and acid phosphatase activity retumed to normal in kidney and liver tissue homogenates after fish and olive oil treatment. In this study, it was found that co-treatment of fish and olive oil is more effective antagonist of gentamicin induced nephrotoxicity. However fish oil was found to be more effective. Hypercholesteromia associated with gentamicin induced nephrotoxicity is also lowered by oil supplementations. The beneficial effects of these oils are due to counteracting effect of the biochemical alterations induced by the drug.

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References

  1. Lee, S. M., Pattison, M. E., Michael, U. F., (1987) Nitrendipine protects against aminoglycoside nephrotoxicity in rats. J. of cardiovascu. Pharmacol. 9, Suppl 1, S65-S69.

    CAS  Google Scholar 

  2. Mathew, T. H., (1992) Drug induced renal disease. Med. J. of Australia. 156, 724–728.

    CAS  Google Scholar 

  3. Beauchamp, D., Gourde, and Bergeron, M. G., (1991) Subcellular distribution of gentamycin in proximal tubular cells, determined by immunogold labeling. Antimicrobial Agents and Chemotherapy. 35, 2173–2179. App. Pharmacol. 86, 271–285.

    PubMed  CAS  Google Scholar 

  4. Silverblatt, F. J., and Knehn, C., (1979) Autobiography of gentamycin uptake by the rat proximal tubule cells. Kidney Int. 15, 335–345.

    Article  PubMed  CAS  Google Scholar 

  5. Laurent, G., M. B. Carlier., B. Rollmann., F. Vantthoff., and P. M. Tulkens., (1982) Mechanism of aminoglycoside induced lysosomal phospholipidosis: in vitro and in vivo studies with gentamicin and amikacin. Biochem. Pharmacol. 31, 3861–3870.

    Article  PubMed  CAS  Google Scholar 

  6. Ginliano, R.A., G.J. Paulus., G.A. Verpooten., V. Pattyn., D.E. Pollet., P.M. Tulkens., and M.E. deBroe., (1984) Recovery of cortical phospholipidosis and necrosis after acute gentamicin loading in rats. Kidney Int. 26, 838–847.

    Article  Google Scholar 

  7. Beauchamp, D., M. Pellerin., P. Gourde., M. Pettigrew, and M. G. Bergeron., (1990) Effects of daptomycin and vancomycin on tobramycin nephrotoxicity in rats. Antimicrobial. Ag. Chemotherapy. 34, 139–147.

    CAS  Google Scholar 

  8. Beauchamp, D., G. Laurent., P. Maldagne., S. Abid., B.K. Kishore., and P.M. Tulkens., (1990) Protection against gentamicin induced early renal alterations (Phospholipidosis and increased DNA synthesis) by co-administration of poly-L-aspartic acid. J. of Pharmacol. Exp. Therapy. 255, 858–866.

    CAS  Google Scholar 

  9. Beauchamp, D., G. Theriault., L. Grenier. P. Gourde., S. Perron., Y. Bergeron., L. Fontaine., and M.G. Bergeron., (1994) Ceftriaxone protects against tobramicin nephrotoxicity. Antimicrobial Ag. Chemotherapy. 38, 750–756.

    CAS  Google Scholar 

  10. Speck, R. F., Lauterberg, B.H., (1999) Fish oil protects mice against acetaminophen hepatotoxicity in vivo. Hepatology. 13, 557–561.

    Google Scholar 

  11. Leung, F. W., (1992) Fish oil protects against ethanol-induced gastric mucosal injury in rats. Digestive Diseases and Sciences. 37, 636–637.

    Article  PubMed  CAS  Google Scholar 

  12. Clark, W. F., Parbtani, A., Naylor, D., Levinton, C. M., Muirhead, N., Spanner, E., Hugg, M. W., Philbrick, D. J., Holub, B. J., (1993) Fish oil in lupus nephritis—clinical findings and methodological Implications. Kidney Int. 44, 75–86.

    Article  PubMed  CAS  Google Scholar 

  13. Ali, B. H., Bashir, A. A., (1994) Effect of fish oil treatment on Gentamicin nephrotoxicity in rats. Annals of Nutr. Meta. 38, 336–339.

    Article  CAS  Google Scholar 

  14. Fingerhunt, B., Ferzola, R., Marsh, W.H., Miller, A.B Jr. (1966) Automated methods for blood glucose and urea with adaptation for simultaneous determination. Clin. Chem. Sep. 12(9), 570–576.

    Google Scholar 

  15. Bonses, R.M., and Taussky, H.H., (1945) On colorimetric determination of creatinine by Jaffe reaction. J. Biol. Chem. 158, 581–591.

    Google Scholar 

  16. Wybenga, D. R., Pillegi, V. J., Dirstine, D. H., Digiorgio, J., (1970) Direct manual determination of serum total cholesterol with a single stable reagent. Clin. Chem. 16, 980–984.

    PubMed  CAS  Google Scholar 

  17. Reitman, S., Frankel, S., (1975) Colorimetric method for the determination of serum glutamic oxaloacetic and gutamic transaminases. Am. J. Clin. Path. 28, 56–63.

    Google Scholar 

  18. Shah, S. V., Kempson, S.A., North Rap, T.E., and Dousa, T. P., (1979) Renal adaptation to a low phosphate diet in rats. J. Clin. Investigation. 64, 955–966.

    Article  CAS  Google Scholar 

  19. Verjee, Z. H. M., (1969) Isolation of three acid phosphatases from wheat germ. Eur. J. of Biochem. 9, 439–444.

    Article  CAS  Google Scholar 

  20. Bennet, C.A., (1967) Statistical analysis in Chemistry and Chemical Industry, New York: John Wiley Sons Inc, p 105.

    Google Scholar 

  21. Morio, saito., Kazuhiro Kubo., (2003) Relationship between tissue lipid per oxidation and peroxidizability index after α-linolinic, eicosapentaenoic, or docosahexaonoic acid intake in rats. Br. J. Nutr. 89. 19–28.

    Article  PubMed  CAS  Google Scholar 

  22. Connor, W.E., (2000) Importance of n-3 fatty acids in health and disease. Am. J. Clin. Nutr. 71, 171S-175S.

    PubMed  CAS  Google Scholar 

  23. Lietman, P. S., Smith, C. R., (1983) Aminoglycoside nephrotoxicity in humans. Rev. Infect. D.S. 5, (Supp.2): 284–290

    CAS  Google Scholar 

  24. Neugarten, F., Aunedjian, H. S., Bank, N. (1983) Role of tubular obstruction in acute renal failure due to gentamycin. Kidney Int. 24, 330–335

    Article  PubMed  CAS  Google Scholar 

  25. Valentina Ruiz-Gutierrez., Alonso Perez-Espinosa., Carmen Maria Vezquez., Consuelo Santa-Maria., (1999) Effect of dietary fats (fish, olive and high oleic oil acid sunflower oils) on lipids composition and antioxidant enzymes in rats liver. Br. J. Nutr. 82, 233–241.

    PubMed  CAS  Google Scholar 

  26. Herold, P. M., Kinsella, J. E., (1986) Fish oil comsumption and decreased rich of cardiovascular disease: a comparison of findings from animals and human feeding trials. Am. J. Clin. Nutr. 43, 566–598.

    PubMed  CAS  Google Scholar 

  27. Mortensen, A., Hansen, B. F., Hansan, J. F., (1998) Comparison of effects of fish oil and olive oil on blood lipids on blood lipids and arotic atherosclerosis in watanabe heritable hyperlipidemic rabbits. Br. J. Nutr. 80 (6), 565–573.

    PubMed  CAS  Google Scholar 

  28. Sheema., M. Kaleem, B. Bano., Effect of dietary n-3 and n-6 fatty acids on tobramicin induced nephrotoxicity: Clinical fingdings. Ind. J. Exp. Biol. (In press).

  29. Bang, H. O., Dyerberg, J., Nielson, A. B., (1986) Plasma lipid and lipoprotein pattern in Groenlandic west coast Eskimos. Nutr. Rev. 44, 143–146.

    Article  Google Scholar 

  30. Miret, S., Saiz, M.P., Mitjavila, M. T., (2003) Effect or fish oil and olive rich-diet on iron matobolism and oxidative stress in the rats. Br. J. Nutr. 89, 11–18.

    Article  PubMed  CAS  Google Scholar 

  31. Bendush, C. L., Weber, R., (1976) Tobramycin sulphate: a summary of worldwide experience from clinical trials. J. infect. Dis (suply) 134, 219–230.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Life Sciences, AMU, 202002, Aligarh, U.P, India

    Fouzia Rashid, M. Kaleem,  Sheema & B. Bano

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  1. Fouzia Rashid
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  2. M. Kaleem
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Correspondence to B. Bano.

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Rashid, F., Kaleem, M., Sheema et al. Comparative effect of olive oil and fish oil supplementation in combating gentamicin induced nephrotoxicity in rats. Indian J Clin Biochem 20, 109–114 (2005). https://doi.org/10.1007/BF02893053

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