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Virchows Archiv B

, Volume 39, Issue 1, pp 259–266 | Cite as

Epidermal proliferation characteristics are similar in the pilary canal of mouse hair follicles and in interfollicular epidermis

  • O. P. F. Clausen
  • E. Thorud
  • K. Elgjo
Article

Summary

Proliferation characteristics of basal cells in the pilary canal of resting hair follicles were investigated and compared with corresponding parameters in interfollicular epidermis of hairless mice. The mitotic rates had similar 24-h means at both locations. Distinct circadian rhythms which showed phasing and amplitudes similar to that in interfollicular epidermis, were demonstrated by the3H-TdR labelling index, the mitotic rate and the mitotic index. Influx of cells to and efflux of cells from the S phase were measured in the early morning and in the evening by a3H-TdR double labelling method. The influx values were similar at both times of both locations. The efflux values recorded in the morning were more than twice the values seen in the evening in both the pilary canal and in interfollicular epidermis. The epidermal mitotic rate in the pilary canal was depressed by epidermal extracts, and increased after adhesive tape stripping in the same way as in interfollicular epidermis. The results indicate no heterogeneity in cell proliferation characteristics between the two locations, and suggest that similar mechanisms are responsible for maintainance of growth equilibrium at both sites.

Key words

DNA synthesis Cell division Epidermis Hair follicles Hairless mice 

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References

  1. Aarnæs E, Thorud E, Clausen OPF (1981) Model analysis of circadian rhythms in mouse epidermal basal cell proliferation. J Theor Biol 88:355–370PubMedCrossRefGoogle Scholar
  2. Al-Bawari SE, Potten CS (1976) Regeneration and dose-response characteristics of irradiated mouse dorsal epidermal cells. Int J Radiat Biol 30:201–216CrossRefGoogle Scholar
  3. Bishop GH (1945) Regeneration after experimental removal of skin in man. Am J Anat 76:153–181CrossRefGoogle Scholar
  4. Bullough WF, Laurence EB (1958) The mitotic activity of the follicle. In: Montagna W, Ellis RA (eds) The biology of hair growth. Academic Press, New York, pp 171–187Google Scholar
  5. Cattaneo SM, Quastler H, Sherman FG (1961) Proliferative cycle in the growing hair follicle of the mouse. Nature 190:923PubMedCrossRefGoogle Scholar
  6. Clausen OPF (1979) Regenerative proliferation of mouse epidermal cells following application of a skin irritant (Cantharidin). Cell Tissue Kinet 12:135–144PubMedGoogle Scholar
  7. Clausen OPF, Lindmo T (1976) Regenerative proliferation of mouse epidermal cells following adhesive tape stripping. Cell Tissue Kinet 9:573–587PubMedGoogle Scholar
  8. Clausen OPF, Lindmo T, Sandnes K, Thorud E (1976) Separation of mouse epidermal basal and differentiating cells for microflow fluorometric measurements. Virchows Arch [Cell Pathol] 20:261–275Google Scholar
  9. Clausen OPF, Thorud E, Bjerknes R, Elgjo K (1979) Circadian rhythms in mouse epidermal basal cell proliferation. Variations in compartment size, flux and phase duration. Cell Tissue Kinet 12:319–337PubMedGoogle Scholar
  10. Clausen OPF, Thorud E, Aaræs E (1981) Evidence of rapid and slow progression of cells through G2 phase in mouse epidermis: A comparison between phase duration measured by different methods. Cell Tissue Kinet 14:227–240PubMedGoogle Scholar
  11. David LT (1932) The external expression and comparative dermal histology of hereditary hairlessness in mammals. Ztsch Zellforsch Mikrobiol Anat 14:616–719CrossRefGoogle Scholar
  12. Devik F (1962) Studies on the duration of DNA synthesis and mitosis in irradiated and regenerating epidermis cells in mice, by means of tritium-labelled thymidine. Int J Radiat Biol 5:59–66PubMedCrossRefGoogle Scholar
  13. Elgjo K, Clausen OPF, Thorud E (1981) Epidermis extracts (chalone) inhibit cell flux at the G1-S, S-G2 and G2-M transitions in mouse epidermis. Cell Tissue Kinet 14:21–29PubMedGoogle Scholar
  14. Hennings H, Elgjo K (1970) Epidermal regeneration after cellophane tape stripping of hairless mouse skin. Cell Tissue Kinet 3:243–252PubMedGoogle Scholar
  15. Iversen OH, Evensen A (1962) Experimental skin carcinogenesis in mice. Acta Pathol Microbiol Scand (Suppl) 156:13Google Scholar
  16. Iversen OH, Bjerknes R, Devik F (1968) Kinetics of cell renewal, cell migration and cell loss in the hairless mouse dorsal epidermis. Cell Tissue Kinet 1:351–367Google Scholar
  17. Iversen U, Iversen OH (1967) Cycles of hair growth in hairless mice. Acta Pathol Microbiol Scand 69:50–62Google Scholar
  18. Kreyberg L, Evensen A, Iversen OH (1965) Influence of stress on the diural rhythm in the mitotic activity in the epidermis of hairless mice. Acta Pathol Microbiol Scand 64:176–184PubMedGoogle Scholar
  19. Laerum OD (1969) Oxygen consumption of basal and differentiating cells from hairless mouse epidermis. J Invest Dermatol 52:204–211PubMedGoogle Scholar
  20. Moffat GH (1968) The kinetics of cell populations in the growing hair follicle of the mouse as revealed by autoradigraphy using H-TdR. In: Baccaredda-Boy A, Moretti G, Fry JR (eds) Biopathology of pattern alopecia. Karger, New York, pp 90–106Google Scholar
  21. Potten CS (1971) Tritiated thymidine incorporation into hair follicle matrix and epidermal basal cells after stimulation. J Invest Dermatol 56:311–317PubMedCrossRefGoogle Scholar
  22. Thorud E, Clausen OPF, Bjerknes R, Aarnæs E (1980) The stathmokinetic method in vivo. Timeresponse with special reference to circadian variations in epidermal cell proliferation in the hairless mouse. Cell Tissue Kinet 13:625–634PubMedGoogle Scholar
  23. Tvermyr EMF (1969) Circadian rhythms in epidermal mitotic activity. Diurnal variations of the mitotic index, the mitotic rate and the mitotic duration. Virchows Arch [Cell Pathol] 2:318–325Google Scholar
  24. Tvermyr EMF (1972) Circadian rhythms in hairless mouse epidermal DNA-synthesis as measured by double labelling with H3-thymidine (H3Tdr). Virchows Arch [Cell Pathol] 11:43–54Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • O. P. F. Clausen
    • 1
    • 2
  • E. Thorud
    • 2
  • K. Elgjo
    • 2
  1. 1.Institute of Forensic MedicineUniversity of OsloOslo 1Norway
  2. 2.Institute of PathologyUniversity of OsloOslo 1Norway

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