Advertisement

Virchows Archiv B

, 39:137 | Cite as

Augmented mitogenesis in normal connective tissue cells following mast-cell secretion in diabetic rats

  • K. Norrby
  • H. J. Arnqvist
  • S. Bergström
  • P. Druvefors
Article

Summary

We studied the proliferative response of fibroblasts and mesothelial cells in the mesentery of streptozotocin-diabetic rats with diabetes of 4 weeks duration, after local mast-cell secretion elicited by compound 48/80. Free peritoneal mast cells from the diabetic animals showed no altered content of 5-HT, histamine, or heparin, and the concentration of histamine in the mesentery and its release by 48/80 were roughly similar in non-diabetic and diabetic animals.

The diabetic animals showed an increased responsiveness in this strictly mast-cell dependent mitogenesis, as measured by specific DNA activity and the mitotic index, regardless of whether the animals were insulin-deficient or treated for 2 h with fast-acting insulin.

Key words

Diabetes Mast cell DNA-synthesis Mitosis 

References

  1. Adamkiewicz VW, Adamkiewicz LM (1960) Glucose and the dextran “anaphylactoid” inflammation. Am J Physiol 198:51–53PubMedGoogle Scholar
  2. Arquilla ER, Weringer EJ, Nakajo M (1976) Wound healing: a model for the study of diabetic angiopathy. Diabetes 25:811–819PubMedCrossRefGoogle Scholar
  3. Elebute EA, Little RA (1978) Effect of streptozotocin-diabetes on the local and general responses to injury in the rat. Clin Sci Mol Med 54:431–4137PubMedGoogle Scholar
  4. Enerbäck L (1974) Berberine sulphate binding to mast cell polyanions: a cytofluorometric method for the quantitation of heparin. Histochemistry 42:301–313PubMedCrossRefGoogle Scholar
  5. Enerbäck L, Mellblom L (1978) 5-hydroxytryptamine content of mast cells, mast cell numbers and body growth. Exp Cell Res 112:103–109PubMedCrossRefGoogle Scholar
  6. Enerbäck L, Wingren U (1980) Histamine content of peritoneal and tissue mast cells in growing rats. Histochemistry 66:113–124PubMedCrossRefGoogle Scholar
  7. Enerbäck L, Franzén L, Norrby K (1976) A tissue model for the study of cell proliferation parameters in vivo. Histochemistry 47:207–218PubMedCrossRefGoogle Scholar
  8. Enerbäck L, Gustafsson B, Mellblom L (1977) Cytofluorometric quantitation of 5-hydroxytryptamine in mast cells: an improved technique for the formaldehyde condensation reaction. J Histochem Cytochem 25:32–41PubMedGoogle Scholar
  9. Franzén L (1981) Further studies on the relationship between drug-induced mast-cell secretion and local cell perliferation. Acta Path Microbiol Scand Sect A 89:57–62Google Scholar
  10. Franzén L, Norrby K (1977) Different proliferative responsiveness of fibroblasts and mesothelial cells in the rat mesentery following administration of compound 48/80 at various hours of the day. Virchows Arch (Cell Pathol) 24:91–99Google Scholar
  11. Franzén L, Norrby K (1980) Local mitogenic effect of tissue mast cells secretion. Cell Tissue Kinet 13:635–642PubMedGoogle Scholar
  12. Franzén L, Norrby K (1982) Immunological challenge causes mitogenic stimulation in normal connective tissue cells. Submitted for publication to Acta Path Microbiol Scand sect AGoogle Scholar
  13. Garcia Lerne J (1981) Regulatory mechanisms on inflammation: new aspects of autopharmacology. Gen Pharmacol 12:15–24Google Scholar
  14. Garcia Lerne J, Hamamura L, Migliorini RH, Leite MP (1973) Influence of diabetes upon the inflammatory response of the rat. A pharmacological analysis. Eur J Pharmacol 23:74–81CrossRefGoogle Scholar
  15. Goldstein S, Harley CB (1979) In vitro studies of age-associated diseases. Fed Proc 38:1862–1867PubMedGoogle Scholar
  16. Goldstein S, Moreman EJ, Soeldner JS, Gleason RE, Barnett DM (1979) Diabetes mellitus and genetic prediabetes. J Clin Invest 63:358–370PubMedCrossRefGoogle Scholar
  17. Goodson WH III, Hunt TK (1977) Studies of wound healing in experimental diabetes mellitus. J Surg Res 22:221–227PubMedCrossRefGoogle Scholar
  18. Goodson WH III, Hunt TK (1979) Deficient collagen formation by obese mice in a standard wound model. Am J Surg 138:692–694PubMedCrossRefGoogle Scholar
  19. Goth A, Nash WL, Nagler M, Holman J (1957) Inhibition of histamine release in experimental diabetes. Am J Physiol 191:25–28PubMedGoogle Scholar
  20. Howard BV, Fields RM, Mott DM, Savage Pj, Nagulesparan M, Bennett PH (1980) Diabetes and cell growth —lack of differences in growth characteristics of fibroblasts from diabetic and non-diabetic Pima Indians. Diabetes 29:119–124PubMedGoogle Scholar
  21. Junod A, Lambert AE, Stauffacher W, Renold AE (1969) Diabetogenic action of streptozotocin ; relationship of dose to metabolic response. J Clin Invest 48:2129–2139PubMedCrossRefGoogle Scholar
  22. Koschinsky T, Bünting CE, Schwippert B, Gries FA (1979) Increased growth of human fibroblasts and arterial smooth muscle cells from diabetic patients related to diabetic serum factors and cell origin. Atherosclerosis 33:245–252PubMedCrossRefGoogle Scholar
  23. Koschinsky T, Bünting CE, Schwippert B, Gries FA (1980) Increased growth stimulation of fibroblasts from diabetics by diabetic serum factors of low molecular weight. Atherosclerosis 37:311–317PubMedCrossRefGoogle Scholar
  24. Llorach MAS, Böhm GM, Garcia Lerne J (1976) Decreased vascular reactions to permeability factors in experimental diabetes. Br J Exp Pathol 57:747–754PubMedGoogle Scholar
  25. Lorenz-Meyer H, Thiel F, Menge H, Gottesbüren H, Riecken EO (1977) Structural and functional studies on the transformation of the intestinal mucosa in rats with experimental diabetes. Res Exp Med 170:89–99CrossRefGoogle Scholar
  26. Movat HZ (1979) The acute inflammatory reaction. In: Movat HZ (ed) Inflammation, immunity and hypersensitivity. Harper & Row, New York, pp 2–161Google Scholar
  27. Nagy D, Redei A, Karady S (1961) Studies on granulation tissue production in alloxan-diabetic rats. J Endocrinol 22:143–146PubMedCrossRefGoogle Scholar
  28. Norrby K (1980) Mast cell histamine, a local mitogen acting via H2-receptors in nearby tissue cells. Virchows Arch (Cell Pathol) 34:13–20Google Scholar
  29. Norrby K (1981) On the 48/80-induced secretion of tissue mast cells and its mitogenic effect on nearby cells in the intact rat. Virchows Arch (Cell Pathol) 38:57–65Google Scholar
  30. Norrby K, Andersson RGG (1979) Early changes of cyclic nucleotide levels in a mitogenic reaction in the rat mesentery. Experientia 35:1623–1624PubMedCrossRefGoogle Scholar
  31. Norrby K, Franzén L (1980) A tissue model for the study of cell proliferation in vitro. In Vitro 16:31–37PubMedCrossRefGoogle Scholar
  32. Norrby K, Enerbäck L, Franzén L (1976) Mast cell activation and tissue cell proliferation. Cell Tissue Res 170:289–303PubMedCrossRefGoogle Scholar
  33. Norrby K, Bergström S, Druvefors P (1981) Age-dependent mitogenesis in normal connective tissue cells. Virchows Arch (Cell Pathol) 36:27–34Google Scholar
  34. Schedl HP, Wilson HD (1971) Effects of diabetes on intestinal growth and hexose transport in the rat. Am J Physiol 220:1739–1745PubMedGoogle Scholar
  35. Seifter E, Rettura G, Padawer J, Stratford F, Kombosos D, Levenson SM (1981) Impaired wound healing in streptozotocin diabetes. Ann Surg 194:42–50PubMedCrossRefGoogle Scholar
  36. Thompson GE (1961) Alloxan and hypersensitivity. Nature 190:822PubMedCrossRefGoogle Scholar
  37. Weiss RE, Reddi AH (1980) Influence of experimental diabetes and insulin on matrix-induced cartilage and bone differentiation. Am J Physiol 238: E 200-E 207Google Scholar
  38. Werner W, Rey H-G, Wielinger H (1970) Über die Eigenschaften eines neuen Chromogens für die Blutzuckerbestimmung nach der GOD/POD-Methode. Z Anal Chem 252:224–228CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • K. Norrby
    • 1
  • H. J. Arnqvist
    • 2
  • S. Bergström
    • 1
  • P. Druvefors
    • 1
  1. 1.Department of Pathology IIUniversity of LinköpingLinköpingSweden
  2. 2.Department of Internal MedicineUniversity of LinköpingLinköpingSweden

Personalised recommendations