Virchows Archiv B

, Volume 38, Issue 1, pp 229–237 | Cite as

Synthesis of Alpha-Fetoprotein (AFP) and cell proliferation in regenerating livers of NMRI mice after partial hepatectomy

An immunohistochemical and autoradiographic study with3H-thymidine
  • H. -V. Tuczek
  • P. Fritz
  • T. Wagner
  • U. Braun
  • A. Grau
  • G. Wegner


To get more information concerning the relation between cell proliferation and the synthesis of alpha-fetoprotein (AFP), liver regeneration and AFP production was followed simultaneously in short intervals in NMRI mice after two-thirds hepatectomy using autoradiographic and immunohistochemical methods. The results indicate that AFP synthesis is not linked closely to cell proliferation in the sense that each proliferating hepatocyte synthesizes AFP during a definite phase of the cell cycle. However, AFP production, as measured by the index of anti-AFP positive hepatocytes, occurs in a very small population of parenchymal cells when the bulk of the hepatocytes has just passed the S-phase and is considered to be in the G2−, M or early G1-phase of the cell cycle when the cells are still in an undifferentiated state.

Key words

α-Fetoprotein Cell proliferation Partial hepatectomy Immunohistochemistry Autoradiography 


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • H. -V. Tuczek
    • 1
  • P. Fritz
    • 1
  • T. Wagner
    • 1
  • U. Braun
    • 1
  • A. Grau
    • 1
  • G. Wegner
    • 1
  1. 1.Department of Clinical and Experimental PathologyRobert-Bosch-HospitalStuttgart 50Federal Republic of Germany

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