Virchows Archiv B

, 37:245 | Cite as

Changes in cyclic nucleotide levels and phosphodiesterase and protein kinase activities in mitogenically stimulated 3T3 fibroblasts

  • R. G. G. Andersson
  • J. F. Kuo
  • K. Norrby
  • J. Wikberg


Activities of cyclic-nucleotide-hydrolysing enzymes cAMP-, cCMP- and cGMP phosphodiesterase, the intracellular concentrations of cAMP, cCMP, and cGMP, and the activity of the cAMP-dependent protein kinase were studied in serum-starved 3T3 cells stimulated to proliferate by serum. Within 1 and 2 min after stimulation the activities of cAMP- and cGMP phosphodiesterase were unaffected while the concentration of cGMP was raised and that of cAMP lowered, suggesting increased synthesis of cGMP and simultaneously reduced synthesis of cAMP. 48 h after stimulation, when the cells multiplied rapidly, both the cAMP phosphodiesterase and the cCMP phosphodiesterase were reduced. Evidence was also obtained that cAMP-dependent potein kinase is important for expressing the cAMP effect in the 3T3 cells.

Key words

Mitogenesis Serum Cyclic Nucleotides Fibroblasts 3T3 cells 


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • R. G. G. Andersson
    • 1
  • J. F. Kuo
    • 2
  • K. Norrby
    • 3
  • J. Wikberg
    • 1
  1. 1.Departments of PharmacologyUniversity of LinköpingSweden
  2. 2.Department of PharmacologyEmory University School of MedicineAtlantaUSA
  3. 3.Department of Pathology IIUniversity of LinköpingLinköpingSweden

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