Light and immunoelectronmicroscopic study of Hodgkin’s disease: Evidence of immunoglobulin synthesis by tumor cells
- 15 Downloads
The direct immunoperoxidase technique with peroxidase-conjugated F(ab′)2 fragments was used at the light and electron microscopic levels to identify intracytoplasmic immunoglobulin (CIg) components in malignant cells of Hodgkin’s disease. In each of the 27 cases studied, Hodgkin and Reed-Sternberg cells contained either IgG or IgM, with both light chains often present simultaneously. The number of IgG-positive malignant cells was inversely related to changes in the lymphoid compartment, as defined by the Rye grading system. The evolution from lymphocytic predominance to lymphocytic depletion was paralleled by a decrease of IgM-positive cells and by a substantial increase (to exclusiveness) of IgG-containing cells. These immunoelectronmicroscopic studies disclosed definite morphologic evidence of CIg synthesis by Hodgkin, Reed-Sternberg and lacunar cells. The immunoglobulin components were also synthesized by lymphoid B cells at different levels of modulation. Immunoglobulin synthesis by malignant cells was localized in perinuclear zone, on free cytoplasmic ribosomes and profiles of rough endoplasmic reticulum. The results of this joint light and electron microscopic study support the view that Hodgkin, Reed-Sternberg and lacunar cells belong to the B-cell compartment within Hodgkin’s disease.
Key wordsHodgkin’s disease Immunoglobulins Immunoperoxidase
Unable to display preview. Download preview PDF.
- Kadin, M.E., Stites, D.P., Levy, R., Warnke, R. (1978) Exogenous immunoglobulin and the macrophage origin of the Reed-Sternberg cells in Hodgkin’s disease. N. Engl. J. Med. 30: 1208–1214Google Scholar
- Landaas, O., Godal, T., Halvorsen, T. (1977) Characterization of immunoglobulins in Hodgkin’s cells. Br. J. Cancer 20: 717–722Google Scholar
- Lennert, K., Kaiserling, E., Müller-Hermelink, H.K. (1978) Malignant lymphomas: Models of differentiation and cooperation of lymphoreticular cells. In: Clarkson, B., Marks, P.A., Till, J.E. (eds) Differentiation of normal and neoplastic hematopoietic cells, book, A. Cold Spring Harbor Conferences on Cell Proliferation, vol. 5, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 897–913Google Scholar
- Lukes, R.J., Craver, L.F., Haal, T.C., Rappaport, H., Ruben, P. (1966) Report of the nomenclature committee. Cancer Res. 26: 1311–1316Google Scholar
- Lukes, R.J., Tindle, B.H. (1978) Immunoblastic lymphadenopathy: a prelymphomatous state of immunoblastic sarcoma. Rec. Results Cancer Res. 64: 241–246Google Scholar
- Rappaport, H. (1966) Tumors of the hematopoietic system. In: Atlas of tumor pathology, Section 3, Fasc. 8, Armed Forces Institute of Pathology, Washington, D.C.Google Scholar
- Seligmann, M., Preud’homme, J.L., Brouet, J.C. (1978) Membrane markers in human lymphoid malignancies: clinicopathological correlations and insight into differentiation of normal and neoplastic cells. In: Clarkson, B., Marks, P.A., Till, J.E. (eds) Differentiation of normal and neoplastic hematopoietic cells. Book A. Cold Spring Harbor Conferences on Cell Proliferation, vol. 5, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 859–876Google Scholar
- Stein, H., Papadimitriou, C.S., Bouman, H., Lennert, K., Fuchs, J. (1978) Demonstration of immunoglobulin production by tumor cells in non-Hodgkin and Hodgkin malignant lymphomas and its significance for their classification. Rec. Res. Cancer Res. 64: 158–175Google Scholar
- Taylor, C.R. (1978) Upon the nature of Hodgkin’s disease and the Reed-Sternberg cell. Rec. Results Cancer Res. 64: 214–231Google Scholar