Biologia Plantarum

, 38:633 | Cite as

Protein and chlorophyll contents in autotrophically and heterotrophically cultivated tobacco mesophyll protoplasts

  • M. Šindelářová
  • L. Burketová
  • L. Šindelář
Brief Communication


Changes in the number of protoplasts, viability, protein and chlorophyll content were studied in tobacco mesophyll protoplasts cultivated either autotrophically in CPW medium with mannitol (MCPW) in the light or heterotrophically in CPW medium with glucose (GCPW) in the dark. The number and viability of protoplasts in the both cultivation media were unchanged. In MCPW in the light, the protein and chlorophyll content strongly decreased already after 12 h of cultivation, at 72 h of cultivation, values dropped to 23.6 % (proteins) and to 3.5 % (chlorophyll) in comparison with the initial content. In GCPW in the dark, the protein and chlorophyll contents decreased only slightly to 75 % (proteins) and to 57.7 % (chlorophyll).

key words

Nicotiana tabacum L. glucose mannitol viability 


  1. Arnon, D.I.: Copper enzymes in isolated chloroplasts: polyphenol oxidase inBeta vulgaris. - Plant Physiol.24: 1–5, 1949.PubMedCrossRefGoogle Scholar
  2. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem.72: 248–254, 1977.CrossRefGoogle Scholar
  3. Cocking, E.C., Peberdy, J.F. (ed.): The Use of Protoplasts from Fungi and Higher Plants as Genetic Systems. A Practical Handbook. - University of Nottingham, Nottingham 1974.Google Scholar
  4. Hooley, R., McCarthy, D.: Extracts from virus infected hypersensitive tobacco leaves are detrimental to protoplast survival. - Physiol. Plant Pathol.16: 25–38, 1980.Google Scholar
  5. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant.15: 473–497, 1962.CrossRefGoogle Scholar
  6. Šindelářová, M., Šindelář, L.: Subceliular localization of ribonucleases in tobacco mesophyll protoplasts and its changes induced by infection of PVY. - Biol. Plant.36: 461–467, 1994.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • M. Šindelářová
    • 1
  • L. Burketová
    • 1
  • L. Šindelář
    • 1
  1. 1.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicPraha 6Czech Republic

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