Protein and chlorophyll contents in autotrophically and heterotrophically cultivated tobacco mesophyll protoplasts
Changes in the number of protoplasts, viability, protein and chlorophyll content were studied in tobacco mesophyll protoplasts cultivated either autotrophically in CPW medium with mannitol (MCPW) in the light or heterotrophically in CPW medium with glucose (GCPW) in the dark. The number and viability of protoplasts in the both cultivation media were unchanged. In MCPW in the light, the protein and chlorophyll content strongly decreased already after 12 h of cultivation, at 72 h of cultivation, values dropped to 23.6 % (proteins) and to 3.5 % (chlorophyll) in comparison with the initial content. In GCPW in the dark, the protein and chlorophyll contents decreased only slightly to 75 % (proteins) and to 57.7 % (chlorophyll).
key wordsNicotiana tabacum L. glucose mannitol viability
- Cocking, E.C., Peberdy, J.F. (ed.): The Use of Protoplasts from Fungi and Higher Plants as Genetic Systems. A Practical Handbook. - University of Nottingham, Nottingham 1974.Google Scholar
- Hooley, R., McCarthy, D.: Extracts from virus infected hypersensitive tobacco leaves are detrimental to protoplast survival. - Physiol. Plant Pathol.16: 25–38, 1980.Google Scholar
- Šindelářová, M., Šindelář, L.: Subceliular localization of ribonucleases in tobacco mesophyll protoplasts and its changes induced by infection of PVY. - Biol. Plant.36: 461–467, 1994.Google Scholar