Biologia Plantarum

, Volume 38, Issue 4, pp 605–609 | Cite as

Hardening by abscisic acid of tobacco plantlets grownin vitro

  • J. PosplŠllová
Brief Communication


Tobacco plantlets were grownin vitro on Murashige and Skoog’s medium with2 % of saccharose. Addition of 0.01 mM abscisic acid (ABA) into the medium decreased stomatal conductance of the adaxial epidermis and especially the abaxial epidermis without negative effects on growth parameters. As a result the rate of water loss from ABA-treated plantlets taken out of cultivation vessels was slower than that of control plantlets. This could help their acclimation after transplantation toex vitro conditions.

Additional key words

Nicotiana tabacum stomatal conductance transpiration curves 



abscisic acid



stomatal conductance


transpiration rate


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  1. Buddendorf-Joosten, J.M.C., Woltering, E.J.: Components of the gaseous environment and their effects on plant growth and development in vitro. - Plant Growth Regul.15: 1–16. 1994.CrossRefGoogle Scholar
  2. Capellades, M., Fontarnau, R., Carulla, C., Debergh, P.: Environment influences anatomy of stomata and epidermal cells in tissue-culturedRosa multiflora. - J. amer. Soc. hort. Sci.115: 141–145, 1990.Google Scholar
  3. Cassells, A.C., Walsh, C: The influence of gas permeability of the culture lid on calcium uptake and stomatal function inDianthus microplants. - Plant Cell Tissue Organ Cult.37:171–178, 1994.CrossRefGoogle Scholar
  4. Colonguasp, W., Nell, T.A., Kane, M.E., Barrett, J.E.: Effects of abscisic acid onex vitro acclimatization ofAronia arbutifolia (L.) Pers..- J. amer. Soc. hort. Sci.121: 101–104, 1996.Google Scholar
  5. Davies, W.J., Jones, H.G.: Abscisic Acid. Physiology and Biochemistry. - BIOS Scientific Publishers, Oxford 1991.Google Scholar
  6. Deng, R., Donnelly, D.J.:In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium. - Can. J. Plant Sci.73: 1105–113, 1993.Google Scholar
  7. Eberhardt, H.-J., Wegmann, K.: Effect of abscisic acid and proline on adaptation of tobacco callus cultures to salinity and osmotic shock. - Physiol. Plant.76: 283–288, 1989.Google Scholar
  8. Eliasson, M.K., Beyl, C.A., Barker, P.A.:In vitro responses and acclimatizationof Prunus serotina with paclobutrazol. - J. Plant Growth Regul.13: 137–142, 1994.CrossRefGoogle Scholar
  9. Emons, A.M.C., Samallo-Droppers, A., Van der Toorn C: The influence of sucrose, mannitol, L-proline, abscisic acid and gibberellic acid on the maturation of somatic embryos ofZea mays L. from suspension cultures. - J. Plant Physiol.142: 597–604, 1993.Google Scholar
  10. Etienne, H., Montoro, P., Michaux-Ferriere, N., Carron, M.P.: Effect of desiccation, medium osmolality and abscisic acid on the maturation ofHevea brasiliensis somatic embryos. - J. exp. Bot.44: 1613–1619, 1993.CrossRefGoogle Scholar
  11. Ghashghaie, J., Brenckmann, F., Saugier, B.: Water relations and growth of rose plants culturedin vitro under various relative humidities. - Plant Cell Tissue Organ Cult.30: 51–57, 1992.CrossRefGoogle Scholar
  12. Kozai, T., Smith, M.A.L.: Environmental control in plant tissue culture - general introduction and overview. - In: Aitken-Christie, J., Kozai, T., Smith, M.L. (ed.): Automation and Environmental Control in Plant Tissue Culture. Pp. 301–318. Kluwer Academic Publishers, Dordrecht - Boston - London 1995.Google Scholar
  13. Misra, S., Attree, S.M., Leal, I., Fowke, L.C.: Effect of abscisic acid, osmoticum, and desiccation on synthesis of storage proteins during the development of white spruce somatic embryos. - Ann. Bot.71: 11–22,1993.CrossRefGoogle Scholar
  14. Pospišilová, J.: Effect of air humidity on the development of functional stomatal apparatus. - Biol. Plant.38: 197–204, 1996.CrossRefGoogle Scholar
  15. Pospišilová, J., Čatský, J., Šestäk, Z.: Photosynthesis in plants cultivatedin vitro. -In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 525–540. Marcel Dekker, New York 1996.Google Scholar
  16. Pospišilová, J., Čatský, J., Synková, H., Macháčková, I., Solárovä, J.: Gas exchange andin vivo chlorophyll fluorescence in potato and tobacco plantletsin vitro as affected by various concentrations of 6-benzylaminopurine. - Photosynthetica29: 1–12, 1993.Google Scholar
  17. Pospišilová, J., Solárovä, J., Čatský, J.: Photosynthetic responses to stresses duringin vitro cultivation. - Photosynthetica26: 3–18, 1992.Google Scholar
  18. Roberts, A.V., Smith, E.F., Mottley, J.: The preparation of micropropagated plantlets for transfer to soil without acclimatization.- In: Pollard, J.W., Walker, J.M. (ed.): Methods in Molecular Biology. Vol. 6. Plant Cell and Tissue Culture. Pp. 227–236. Humana Press, Clifton 1990.CrossRefGoogle Scholar
  19. Sallanon, H., Maziere, Y.: Influence of growth room and vessel humidity on thein vitro development of rose plants. -Plant Cell Tissue Organ. Cult.30: 121–125, 1992.CrossRefGoogle Scholar
  20. Short, K.C., Warburton, J., Roberts, A.V.:In vitro hardening of cultured cauliflower andChrysanthemum plantlets to humidity. - Acta Hort.212: 329–334, 1987.Google Scholar
  21. Smith, E.F., Roberts, A.V., Mottley, J.: The preparationin vitro chrysanthemum for transplantation to soil. 3. Improved resistance to desiccation conferred by reduced humidity. - Plant Cell Tissue Organ Cult.21: 141–145, 1990.CrossRefGoogle Scholar
  22. Smith, E.F., Gribaudo, I., Roberts, A.V., Mottley, J.: Paclobutrazol and reduced humidity improve resistance to wilting of micropropagated grapevine. - HortScience27: 111–113, 1992.Google Scholar
  23. Solárová, J., Součková, D., Ullmann, J., Pospišilová, J.:In vitro culture: environmental conditions and plantlet growth as affected by vessel type and stopper material. - Zahradnictvi23(2): 51–58, 1996.Google Scholar
  24. Wardle, K., Dobbs, E.B., Short, K.C.:In vitro acclimatization of aseptically cultured plantlets to humidity. - J. amer. Soc. hort. Sci. 108: 386–389, 1983.Google Scholar
  25. Yue, D., Gosselin, A., Desjardins, Y.: Effect of forced ventilation at different relative humidities on growth, photosynthesis and transpiration of geranium plantletsin vitro. - Can. J. Plant Sci.73: 249–256, 1993.Google Scholar
  26. Zaid, A., Hughes, H.: Water loss and polyethylene glycol-mediated acclimatization ofin vitro-grown seedlings of 5 cultivars of date palm(Phoenix dactylifera L.) plantlets. - Plant Cell Rep.14: 385- 388, 1995.CrossRefGoogle Scholar
  27. Ziv, M.:In vitro hardening and acclimatization of tissue culture plants. - In: Withers, L.A., Alderson, P.G. (ed.): Plant Tissue Culture and its Agricultural Applications. Pp. 187–196. Butterworths, London - Boston - Durban -Singapore - Sydney - Toronto - Wellington 1986.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • J. PosplŠllová
    • 1
  1. 1.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicCzech Republic

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