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Biologia Plantarum

, Volume 38, Issue 4, pp 605–609 | Cite as

Hardening by abscisic acid of tobacco plantlets grownin vitro

  • J. PosplŠllová
Brief Communication

Abstract

Tobacco plantlets were grownin vitro on Murashige and Skoog’s medium with2 % of saccharose. Addition of 0.01 mM abscisic acid (ABA) into the medium decreased stomatal conductance of the adaxial epidermis and especially the abaxial epidermis without negative effects on growth parameters. As a result the rate of water loss from ABA-treated plantlets taken out of cultivation vessels was slower than that of control plantlets. This could help their acclimation after transplantation toex vitro conditions.

Additional key words

Nicotiana tabacum stomatal conductance transpiration curves 

Abbreviations

ABA

abscisic acid

BAP

6-benzylaminopurine

stomatal conductance

E

transpiration rate

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • J. PosplŠllová
    • 1
  1. 1.Institute of Experimental BotanyAcademy of Sciences of the Czech RepublicCzech Republic

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