Immunocytochemical characterization of S-100β-positive human T-lymphocytes by a double immunostaining method
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The antigenic properties of S-100β-positive human T-lymphocytes (S-100β+ T-cells) were investigated by a double immunostaining technique employing an indirect immunoperoxidase method for cytoplasmic S-100β subunit and an immunoalkaline phosphatase method for cell surface antigens detected by various monoclonal antibodies to human lymphocytes. S-100β+ T-cells recognized by their diffuse intracytoplasmic immunoperoxidase reaction, also expressed CD2, CD3, CD8 antigens demonstrated by surface blue alkaline phosphatase reactivity, but not CD4, CD1, CD25 (interleukin-2 receptor), or HLA-DR antigens. However, they displayed a blastic change to T-cell mitogens, such as Concanavalin A(Con-A) and PHA, followed by the expression of CD25 and HLA-DR antigens. Under normal conditions, S-100β+ T-cells comprised approximately 5–22.8% of CD8+ cells amongst human peripheral blood mononuclear cells.
Key wordsS-100b protein CD8+ -T-lymphocyte Monoclonal antibody, and double immunostaining method
- Takahashi K, Yamaguchi H, Ishizeki J, Nakajima T, Nakazato Y (1981) Immunohistochemical and immunoelectron micro- scopic localization of S-100 protein in the interdigitating reticulum cells of the human lymph node. Virchows Arch [Cell Pathol] 37:125–135Google Scholar
- Takahashi K, Isobe T, Ohtsuki Y, Akagi T, Sonobe H, Oku- yama T (1984) Immunohistochemical study on the distribu- tion of α and β subunits of S-100 protein in human neoplasm and normal tissues. Virchows Arch [Cell Pathol] 45:385–396Google Scholar
- Takahashi K, Yoshino T, Hayashi K, Sonobe H, Ohtsuki Y (1987) S-100 beta positive human T-lymphocytes: Their characteristics and behaviour under normal and pathologi- cal conditions. Blood (in press)Google Scholar