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Virchows Archiv B

, 53:191 | Cite as

The establishment and characterization of two new cell lines derived from a single human colonic adenocarcinoma

  • Cees P. H. J. Verstijnen
  • Jan W. Arends
  • Peter T. M. Moerkerk
  • Joep P. M. Geraedts
  • Koji Sekikawa
  • Martin P. Uitendaal
  • Fred T. Bosnian
Article

Summary

Two cell lines with different in vitro growth characteristics were established from a single mucinous colonic adenocarcinoma. Epithelial cells of the line 5583-E demonstrated anchoragedependent growth while those of line 5583-S were anchorage-independent and grew as multicellular floating spheroids. Both cell lines shared common characteristics with respect to the expression of differentiation markers (secretory component, carcinoembryonic antigen), mucins and karyotype (trisomy 12 and 14, marker chromosome) but also showed consistent differences. In nude mice 5583-S cells formed moderately differentiated mucinous adenocarcinomas with high carcinoembryonic antigen and mucin production, whereas 5583-E xenografts were poorly differentiated and almost entirely failed to produce carcinoembryonic antigen and mucins. The plating efficiency of 5583-E cells appeared to be greater and doubling time shorter than those of 5583-S cells. Furthermore, 5583-E cells showed an extra isochromosome, lq. The cell lines were genotypically and phenotypically stable over a period of 2 years. Our results reemphasize that multiple cell lines with heterogeneous phenotypic and genotypic characteristics can be obtained from a single primary tumor.

Key words

Colon carcinoma Cell lines Mucin 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Cees P. H. J. Verstijnen
    • 1
  • Jan W. Arends
    • 1
  • Peter T. M. Moerkerk
    • 1
  • Joep P. M. Geraedts
    • 2
  • Koji Sekikawa
    • 4
  • Martin P. Uitendaal
    • 3
  • Fred T. Bosnian
    • 1
  1. 1.Departments of PathologyUniversity of LimburgMaastrichtThe Netherlands
  2. 2.Departments of Genetics and Cell BiologyUniversity of LimburgMaastrichtThe Netherlands
  3. 3.Departments of PharmacologyUniversity of LimburgMaastrichtThe Netherlands
  4. 4.Department of SurgeryFukushima Medical CollegeJapan

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