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Virchows Archiv B

, 53:13 | Cite as

Freeze-fracture features of epithelioid cells, multinucleated giant cells, and phagocytic macrophages

Investigations using the model of experimental autoimmune (anti-TBM) tubulo-interstitial nephritis
  • Hans -Peter Baum
  • Wolfgang Thoenes
Original Articles

Summary

The freeze-fracture morphology of epithelioid cells, multinucleated giant cells (Langhans’ type), and phagocytic macrophages was investigated. The intensely folded and interdigitating surface membranes of epithelioid cells and multinucleated giant cells displayed no specialized areas of cell contact. The size of the intramembranous particles (IMP) and the fact that the area density of IMPs was higher in the cytoplasmic (P) faces than in the external (E) faces of the cell membranes agreed with observations in other eukaryotic cells. The area densities of the IMPs suggest lower transport rates of molecules across the cell membranes of granuloma cells than of certain epithelial cells. Small pits were detected in the surface membranes of the granuloma cells but an extrusion of granules was not observed. The cytoplasmic granules displayed very different sizes and shapes ranging from spherical to rod-shaped. The latter type of granules (probably primary lysosomes) dominated in multinucleated giant cells. The granule membranes were studded with IMPs whose area densities increased with the granule size. Multilamellar bodies with smooth (lipid) fracture faces were found only in phagocytic macrophages. The nuclear pores of the granuloma cells were distributed over the entire surfaces of the nuclei and displayed moderate clustering. The values of the area densities of the nuclear pores were in keeping with the values observed in mammalian and human epithelial or mesenchymal cells, indicating similar exchange rates of molecules between the nucleoplasm and the cytoplasm in these different cell types.

In a single phagocytic macrophage the E-face of the inner membrane of the nuclear envelope displayed a network of fine filaments whose nature is at present unknown.

Key words

Granuloma Epithelioid cells Multinucleated giant cells Macrophages Freezefracture Tubulo-interstitial nephritis 

References

  1. Adams DO (1983) The biology of granuloma. In: Joachim HL (ed) Pathology of granulomas. Raven Press, New York, pp 1–20Google Scholar
  2. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD (1983) Molecular biology of the cell. Garland, New York LondonGoogle Scholar
  3. Banner BF, Alroy J, Pauli BU, Carpenter JL (1978) An ultra- structural study of acinic cell carcinoma of the canine pan- creas. Am J Pathol 93:165–182PubMedGoogle Scholar
  4. Baum HP, Thoenes W (1985) Differentiation of granuloma cells (epithelioid cells and multinucleated giant cells): a morpho- metric analysis. Investigations using the model of experi- mental autoimmune (anti-TBM) tubulo-interstitial nephri- tis. Virchows Arch [Cell Pathol] 50:181–192CrossRefGoogle Scholar
  5. Bosch M van den, Jacob W, Pattyn S (1980) Age-related evolu- tion of cell membrane invaginations of murine peritoneal macrophages. VII. Eur Congr Electron Microsc Den Haag 2:196–197Google Scholar
  6. Chambers TJ (1978) Multinucleate giant cells. J Pathol 126:125–148PubMedCrossRefGoogle Scholar
  7. Daems WT, Breederoo P (1970) The fine structure of mononu- clear phagocytes as revealed by freeze-etching. In: van Furth R (ed) Mononuclear phagocytes. Blackwell Scientific Publications, Oxford, pp 29–42Google Scholar
  8. Deamer DW, Leonard R, Tardieu A, Branton D (1970) Lamel- lar and hexagonal lipid phases visualized by freeze-etching. Biochim Biophys Acta 219:47–60PubMedCrossRefGoogle Scholar
  9. Epstein WL (1977) Granuloma formation in man. Pathobiol Ann 7:1–30Google Scholar
  10. Fawcett DW (1966) On the occurrence of a fibrous lamina on the inner aspect of the nuclear envelope in certain cells of vertebrates. Am J Anat 119:129–146PubMedCrossRefGoogle Scholar
  11. Fisher HW, Cooper TW (1967) Electron microscope observa- tions on the nuclear pores of HeLa cells. J Cell Biol 35:40AGoogle Scholar
  12. Furth R van, Cohn ZA, Hirsch JG, Humphrey JH, Spector WG, Langevoort HL (1972) The mononuclear phagocyte system: a new classification of macrophages, monocytes, and their precursor cells. Bull WHO 46:845–852PubMedGoogle Scholar
  13. Gusek W (1965) Histologie und elektronenmikroskopische komparative Zytologie tuberkulöser und epitheloidzelliger Granulome. Fortschr Tuberkuloseforsch 14:97–156Google Scholar
  14. Hasty DL, Hay ED (1978) Freeze-fracture studies of the devel- oping cell surface. II. Particle-free membrane blisters on glutaraldehyde-fixed corneal fibroblasts are artefacts. J Cell Biol 78:756–768PubMedCrossRefGoogle Scholar
  15. Kraus B (1980) Mehrkernige Riesenzellen in Granulomen. Verh Dtsch Ges Pathol 64:103–125PubMedGoogle Scholar
  16. Krieger A (1979) Experimentelle autoimmune tubulo-intersti- tielle Nephritis. Inaug Diss, MünchenGoogle Scholar
  17. Langer KH, Thoenes W (1981) Characterization of cells in- volved in the formation of granuloma. An ultrastructural study on macrophages, epithelioid cells, and giant cells in experimental tubulo-interstitial nephritis. Virchows Arch [Cell Pathol] 36:177–194Google Scholar
  18. Langer KH, Thoenes W (1984) Endocytotic activity of epithe- lioid and Langhans’ giant cells. Tracer studies with ferritin in the tubulo-interstitial (anti-TBM) nephritis model. Vir- chows Arch [Cell Pathol] 47:177–182Google Scholar
  19. Maul GG, Deaven L (1977) Quantitative determination of nu- clear pore complexes in cycling cells with different DNA content. J Cell Biol 73:748–761PubMedCrossRefGoogle Scholar
  20. Miyata K, Takaya K (1984) Intercellular junctions between macrophages in the regional lymph node of the rat after injection of large doses of steroids. Cell Tissue Res 236:351–355PubMedCrossRefGoogle Scholar
  21. Müller-Hermelink HK, Kaiserling E (1980) Epitheloidzellreak- tionen im lymphatischen Gewebe. Verh Dtsch Ges Pathol 64:77–102PubMedGoogle Scholar
  22. Orci L, Perrelet A (1975) Freeze-etch histology. Springer, Berlin Heidelberg New YorkGoogle Scholar
  23. Orci L, Amherdt M, Malaisse-Lagae F, Perrelet A, Dulin WE, Gerritsen GC, Malaissse WJ, Renold AE (1974) Morpho- logical characterization of membrane systems in A- and B- cells of the Chinese hamster. Diabetologia 10:529–539PubMedCrossRefGoogle Scholar
  24. Papadimitriou JM, Robertson TA (1980) Exocytosis by macro- phage polykarya: an ultrastructural study. J Pathol 130:75–81PubMedCrossRefGoogle Scholar
  25. Papadimitriou JM, Spector WG (1971) The origin, properties and fate of epithelioid cells. J Pathol 105:187–203PubMedCrossRefGoogle Scholar
  26. Papadimitriou JM, Sforsina D, Papaelias L (1973) Kinetics of multinucleate giant cell formation and their modification by various agents in foreign body reactions. Am J Pathol 73:349–362PubMedGoogle Scholar
  27. Rejthar A, Blumajer J (1974) Difference in density of nuclear pores in normal and malignant fibroblasts of Syrian hamster. Neoplasma 21:479–489PubMedGoogle Scholar
  28. Smith DS, Smith U, Ryan JW (1972) Freeze-fractured lamellar body membranes of the rat lung great alveolar cells. Tissue Cell 4:457–468PubMedGoogle Scholar
  29. Sugisaki T, Klassen J, Milgrom F, Andres GA, McCluskey RT (1973) Immunopathologic study of an autoimmune tu- bular and interstitial renal disease in Brown-Norway rats. Lab Invest 28:658–671PubMedGoogle Scholar
  30. Thoenes W, Langer KH (1969) Die Endocytose-Phase der Ei- weißresorption im proximalen Nierentubulus. Untersuchun- gen am Ferritin-resorbierenden Einzeltubulus der Ratten- niere. Virchows Arch [Cell Pathol] 2:361–379Google Scholar
  31. Thoenes W, Sonntag W, Heine WD, Langer KH (1982) Cell fusion as a mechanism for the formation of giant cells (Langhans’ type). Autoradiographic findings in autoim- mune tubulo-interstitial nephritis of the rat. Virchows Arch [Pathol Anat] 41:45–50Google Scholar
  32. Tipperman R, Kasckow J, Herndon RM (1984) The fine struc- ture of macrophages in lysolecithin-induced demyelination: A freeze-fracture study. J Neuropathol Exp Neurol 43:522–530PubMedGoogle Scholar
  33. van der Rhee HJ, van der Burgh-de Winter CPM, Daems WT (1979) The differentiation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells in subcutane- ous granulomas. I. Fine structure. Cell Tissue Res 197:355–378Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Hans -Peter Baum
    • 1
  • Wolfgang Thoenes
    • 1
  1. 1.Institute of PathologyJohannes-Gutenberg-University of MainzMainzFederal Republic of Germany

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