Virchows Archiv B

, Volume 47, Issue 1, pp 27–33 | Cite as

Morphological effects of transposing a segment of transverse colon into the ileum of the Holtzman rat

  • W. H. St. Clair
  • C. A. Stahlberg
  • J. W. Osborne


In order to determine if the rat colon is capable of adaptation when placed in another location within the intestinal tract and/or subject to different luminal contents, a 3 cm segment of transverse colon was transposed into the ileum. Sixteen days later, the animals were injected with tritiated thymidine (1 μCi/g body weight) and killed one hour later. Autoradiographs were analyzed for number of cells per crypt column, number of labeled cells per crypt column, position of labeled cells in the crypt column, and formation of Paneth cells or villi in the transposed colon. Proliferative activity of the epithelium was measured by isolating whole crypts and determining disintegrations per minute per crypt. Except for alterations in the positions of labeled cells, as determined by crypt profiles, no changes from normal were observed in any of the parameters measured. Hence, unlike the small bowel, the colon is refractory to the influences of a new environment.

Key words

Transposition Ileum Transverse colon Intestinal mucosa 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Altmann GG, Leblond CP (1970) Factors influencing villus size in the small intestine of adult rats as revealed by transposition of intestinal segments. Am J Anat 127:15–36PubMedCrossRefGoogle Scholar
  2. Bloom W, Fawcett DW (1975) Intestines. In: A textbook of histology. WB Saunders Co, Philadelphia, 658–689Google Scholar
  3. Cairnie AB, Bentley RE (1967) Cell proliferation studies in the intestinal epithelium of the rat. Hyperplasia during lactation. Exp Cell Res 46:428–440PubMedCrossRefGoogle Scholar
  4. Celik C, Mittelman A, Paolini NS, Lewis D, Evans JT (1981) Effects of 1,2-symmetrical dimethylhydrazine on jejunocolic transposition in Sprague-Dawley rats. Cancer Res 41:2908–2911PubMedGoogle Scholar
  5. Hagemann RF, Sigdestad CP, Lesher S (1970) A method for quantitation of proliferative intestinal mucosal cells on a weight basis: Some values for C57B1/6. Cell Tissue Kinet 3:21–26PubMedGoogle Scholar
  6. Hanson WR, Osborne JW, Sharp JG (1977) Compensation by the residual intestine after intestinal resection in the rat. II. Influence of postoperative time interval. Gastroenterology 72:701–705PubMedGoogle Scholar
  7. Lewin K (1969) The Paneth cell in disease. Gut 10:804–811PubMedCrossRefGoogle Scholar
  8. Osborne JW, Smit RA (1983) Prior irradiation of the rat small bowel does not affect the proliferative response to intestinal resection. International Congress of Radiation Research. Abstract number D3-33, AmsterdamGoogle Scholar
  9. Philipson B (1975) Morphology in the cat ileal mucosa following construction of an ileal reservoir or transposition of patches to different locations. Scand J Gastroenterol 10:369–377PubMedGoogle Scholar
  10. Potten CS (1980) Stem cells in small-intestinal crypts. In: Appleton DR, Sunter JP, Watson AJ (eds) Cell proliferation in the gastrointestinal tract. Pitman Medical, Tunbridge Wells, pp 141–154Google Scholar
  11. Rijke RPC, Hanson WR, Plaisier HM (1977) The effect of transposition to jejunum on epithelial cell kinetics in an ileal segment. Cell Tissue Kinet 10:399–406PubMedGoogle Scholar
  12. Sandow MJ, Whitehead R (1979) Progress report: The Paneth cell. Gut 20:420–431PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • W. H. St. Clair
    • 1
  • C. A. Stahlberg
    • 1
  • J. W. Osborne
    • 1
  1. 1.Radiation Research Laboratory, Department of RadiologyUniversity of IowaIowa CityUSA

Personalised recommendations