Advertisement

Virchows Archiv B

, 44:241 | Cite as

Elastase-like enzyme in the aorta of spontaneously hypertensive rats

  • Eiji Yamada
  • Fumitada Hazama
  • Hideo Kataoka
  • Shigeru Amano
  • Masakiyo Sasahara
  • Kalula Kayembe
  • Kouichi Katayama
Short Communication

Summary

In an attempt to obtain information regarding vascular elastase in arterial hypertension, we examined biochemical changes in elastase-like enzyme activity, and the intravascular localization of elastase by immunohistochemical techniques in the aorta of spontaneously hypertensive rats (SHR). In the biochemical study, aortic elastase-like enzyme activity was significantly higher in SHR than in controls. Using an antibody against rat pancreatic elastase raised in the rabbit, it was demonstrated immunohistochemically that the enzyme was localized in the endothelial cells and subendothelial spaces in the aorta of control animals. In SHR, elastase was also demonstrated in medial smooth muscle cells and particularly in the modified smooth muscle cells in areas of intimai thickening. Some vacuoles in the smooth muscle cells also showed positive enzyme staining. Elastase seems to play an important role in the development of hypertensive vascular changes.

Key words

Elastase-like enzyme Aorta Spontaneously hypertensive rats 

References

  1. Amano S (1976) Vascular changes in the brain of spontaneously hypertensive rats: hyaline and fibrinoid degeneration. J Pathol 121:119–128CrossRefGoogle Scholar
  2. Bieth J, Spiess B, Wermuth CG (1974) The synthesis and analytical use of highly sensitive and convenient substrate of elastase. Biochem Med 11:350–357PubMedCrossRefGoogle Scholar
  3. Hazama F, Yamada E, Amano S, Watanabe M (1981) Histo- und cytochemical studies of lysosomes in cerebral arteries in spontaneously hypertensive rats: Role of lysosomal enzymes in development of hypertensive cerebrovascular changes. In: Cervos-Navarro J, Fritschka E (eds). Cerebral microcirculation and metabolism. Raven Press, New York, pp 93–98Google Scholar
  4. Hornebeck W, Adnet JJ, Robert L (1978) Age dependent variation of elastin and elastase in aorta and human breast cancers. Exp Geront 13:293–298CrossRefGoogle Scholar
  5. Ito H, Yamamoto K, Okamoto K (1979) The characteristics of aortic elastin in SHRSP. Jpn Heart J 20:Suppl I 240–242Google Scholar
  6. Katayama K, Ooyama T (1980) A simple purification procedure for rat pancreatic elastase and radioimmunoassay of the enzyme. Chem Pharm Bull (Tokyo) 28/11:3422–3426Google Scholar
  7. Loeven WA (1969) Elastolytic enzymes in the vessel wall. J Atheroscler Res 9:35–45PubMedCrossRefGoogle Scholar
  8. Nakao J, Chang W, Murota S, Orimo H (1980) Elastinolytic activity in the rat aortic smooth muscle cells in culture. Atherosclerosis 36:539–544PubMedCrossRefGoogle Scholar
  9. Riede UN, Staubesand J (1977) A unifying concept for the role of matrix vesicles and lysosomes in the formal pathogenesis of diseases of connective tissues and blood vessels. Beitr Pathol 160:3–37PubMedGoogle Scholar
  10. Sternberger LA, Hardy OH, Cuculis JJ, Meyer HG (1970) The unlabeled antibody enzyme method of immunochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti horseradish peroxidase) and its use in identification of spirochates. J Histochem Cytochem 18:315–333PubMedGoogle Scholar
  11. Wolinsky H, Goldfisher S, Schiller B, Kasak LE (1974) Modification of the effects of hypertension on lysosomes and connective tissue in the rat aorta. Circ Res 34:233–241PubMedGoogle Scholar
  12. Yamada E, Hazama F, Amano S, Hanakita J (1980) Cytochemical investigation on acid phosphatase activity in cerebral arteries in spontaneously hypertensive rats. Jpn Circ J 44:467–475PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Eiji Yamada
    • 1
  • Fumitada Hazama
    • 1
  • Hideo Kataoka
    • 1
  • Shigeru Amano
    • 1
  • Masakiyo Sasahara
    • 1
  • Kalula Kayembe
    • 1
  • Kouichi Katayama
    • 2
  1. 1.Department of PathologyShiga University of Medical Science, School of MedicineOtsuJapan
  2. 2.Department of Drug Metabolism, Section of Experimental Therapeutics ResearchResearch and Development Division, Eisai Co., Ltd.Tokyo

Personalised recommendations