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Virchows Archiv B

, 48:341 | Cite as

Tissue distribution and ultrastructure of B lymphocytes reacting with the monoclonal antibody anti-Y29/55

  • F. G. Gudat
  • H. K. Forster
  • F. Suter
  • R. Albrecht
  • G. Krey
  • U. Dürmüller
  • M. -F. Girard
  • J. -P. Obrecht
Article

Summary

The reactivity of normal tonsilar cells with the monoclonal antibody anti-Y29/55 is characterized at the tissue and ultrastructural cytological level. Using an indirect immuno-alkaline phosphatase method on frozen sections the antibody labels mantle zone and germinal center lymphocytes. This staining reaction is more generalized in B-lymphocyte areas than that obtained with antibodies to IgM and IgD. By indirect immunoperoxidase staining, as well as by an indirect rosetting procedure in cell suspensions, the reactive cell population were either small resting lymphocytes or activated lymphocytes corresonding to centrocytes, centroblasts, immunoblasts and plasmoblasts ; some plasma cells were also labeled. These results characterize the monoclonal antibody anti-Y29/55 as a pan-B-marker antibody, useful for labeling resting and activated peripheral B-lymphocytes in frozen tissue sections and cell suspensions.

Kew words

Lymphoid Tissue B-lymphocytes Monoclonal antibodies Immunocytochemistry Electron microscopy 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • F. G. Gudat
    • 1
  • H. K. Forster
    • 2
  • F. Suter
    • 1
  • R. Albrecht
    • 2
  • G. Krey
    • 1
  • U. Dürmüller
    • 1
  • M. -F. Girard
    • 2
  • J. -P. Obrecht
    • 3
  1. 1.Department of PathologyUniversity of BaselBasel
  2. 2.Central Research UnitF. Hoffmann-LaRoche & Co., Ltd.Basel
  3. 3.Immuno-oncological Unit, Department of ResearchKantonsspital BaselBaselSwitzerland

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