Interleukin-6 and its receptor are expressed in human intestinal epithelial cells
- 141 Downloads
Recent studies have suggested that intestinal epithelial cells demonstrate some of the functions associated with immune competent cells. Based on these observations, we investigated whether gastrointestinal epithelial cells express Interleukin-6 (IL-6). The presence of this cytokine was tested in 53 normal and pathological tissue specimens of the human gastrointestinal tract using an immunohistochemical technique with anti-IL-6 monoclonal and polyclonal antibodies. Immunostaining shows that IL-6 is expressed in gastric and small intestinal epithelial cells. The tumor cells from a large subset (11 of 15) of colon cancer specimens were strongly immunostained. IL-6 immunostaining was less conspicuous and less frequent in the epithelial cells of normal colonic mucosa. Northern blot experiments indicated that the expression of IL-6 in colonic mucosa correlates quantitatively with the presence of its m-RNA. Furthermore, IL-6 receptor (IL-6R) m-RNA was also detected and was twice as abundant in colonie carcinoma as in normal colon. It is concluded that mucosal epithelial cells of the gastrointestinal system express IL-6 and that in the case of the colon, malignancy is accompanied by a higher expression. In addition, the presence of IL-6R transcript suggests that normal and neoplastic colonie epithelial cells might be autocrinally regulated by IL-6.
Key wordsInterleukin-6 Epithelial cells m-RNA Immunohistochemistry Colon cancer
Unable to display preview. Download preview PDF.
- Hirano T, Yasukawa K, Harada H, Taga T, Watanabe Y, Matsuda T, Kashiwamura S, Nakajima K, Koyama K, Iwamatu A, Tsunasawa S, Sakiyama F, Matsui H, Takahara Y, Taniguchi T, Kishimoto T (1986) Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature 324:73–76PubMedCrossRefGoogle Scholar
- Kirnbauer R, Koeck A, Schwarz T, Urbanski A, Krutmann J, Broth W, Damm D, Shipley G, Ansel JC, Luger TA (1989) IFN-beta 2, B Cell differentiation factor 2, or hybridoma growth factor (IL-6) is expressed and released by human epiderman cells and epidermoid carcinoma cell lines. J Immunol 142:1922–1928PubMedGoogle Scholar
- Natali PG, DeMartino C, Quaranta V, Nicotra MR, Frezza F, Pelligrino MA, Ferrone S (1981) Expression of la-like antigens in normal human non-lymphoid tissues. Transplantation (Baltimore) 31:75–78Google Scholar
- Sternberger LA, Hardy PH, Cululis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry, preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti-horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333PubMedGoogle Scholar