Virchows Archiv B

, 52:479 | Cite as

Immunohistochemically demonstrated increase in glutathione S-transferase species in propvlnitrosamine-induced focal proliferative and neoplastic Syrian hamster pancreatic lesions

  • Malcolm A. Moore
  • Peter Bannasch
  • Toshikazu Satoh
  • Hans Jörg Hacker
  • Nobuyuki Ito


Immunohistochemical investigation of focal proliferative and neoplastic Syrian hamster pancreatic lesions induced by propylnitrosamine administration revealed a distinct pattern of expression of different molecular forms of glutathione S-transferase (GST) during neoplastic development. Initial increase in levels of GST placental (P) and B forms in early ductal/ductular proliferations and atypical (dysplastic) lesions was followed by a drop in the latter during transition to carcinoma. Unequivocal acinar cells observed within so-called ‘pseudoductules’ did not share the altered phenotype evident in ductular elements, suggesting their non-involvement in the generation of early lesions. However, the fact that component cells were occasionally of abnormal morphology did not allow the exclusion of acinar cell participation in histogenesis. Elevation of GST-A and C forms was limited to stromal elements surrounding the epithelial lesions and since they were associated with benign, cystic as well as atypical lesions and a similar increase was observed after common duct ligation, they appeared to be non-specific. The results indicated independent control of expression of individual GST forms and suggested that biochemical similarities exist between early, putative preneoplastic lesions induced by propylnitrosamines in the hamster lung, liver (both hepatocellular and intrahepatic bile duct) and pancreas.

Key words

Propylnitrosamine carcinogenesis Pancreas Enzyme phenotype Common duct ligation 


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Malcolm A. Moore
    • 1
  • Peter Bannasch
    • 2
  • Toshikazu Satoh
    • 3
  • Hans Jörg Hacker
    • 2
  • Nobuyuki Ito
    • 1
  1. 1.First Department of PathologyNagoya City University Medical SchoolNagoya 467Japan
  2. 2.Division of Cytopathology, Institute for Experimental PathologyGerman Cancer Research CenterHeidelbergFederal Republic of Germany
  3. 3.Second Department of BiochemistryHirosaki University Medical SchoolHirosaki 036Japan

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