Virchows Archiv B

, Volume 52, Issue 1, pp 319–325 | Cite as

Myeloperoxidase deficient polymorphonuclear leucocytes: computerized planimetric estimations of cellular and nuclear size

  • Knud Bendix-Hansen


Quantitative estimations of the mean areas of cell, nucleus and cytoplasm in polymorphonuclear leucocytes (PMN) were performed by automated image analysis of blood smears from six patients with acute myeloid leukaemia. The PMN were qualitatively separated by a cytochemical staining method into two well-defined subpopulations i.e. myeloperoxidase (MPO)-normal and MPO-deficient PMN.

MPO-deficient PMN were characterized by a decreased size of the total cell (P<0.01), an increased size of the nucleus (P<0.01) and a decreased size of the cytoplasm (P<0.01). The resulting highly increased nucleus-to-cytoplasm ratio in this specific PMN subpopulation bears a striking resemblance to cells in malignant tumours. The planimetric results in this study further support the concept that MPO-deficient PMN may be the progeny of leukaemic precursors.

Key words

Acute myeloid leukaemia Computer-assisted planimetry MPO deficient PMN 


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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Knud Bendix-Hansen
    • 1
  1. 1.University Institute of Pathology and University Department of Medicine and HaematologyAarhus CDenmark

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