Advertisement

Virchows Archiv B

, Volume 50, Issue 1, pp 101–108 | Cite as

The influence of castration on cell proliferation in the adrenal cortex of female rats

  • Heinz Ueberberg
  • Jutta Neuss
  • Gernot Trieb
Article
  • 11 Downloads

Summary

Proliferation kinetics in the zones of the adrenal cortex of female rats after castration are reported. Following ovariectomy a wave of increased cell proliferation was observed in the zona glomerulosa and zona fasciculata after 1 and 2 days respectively. Increased cell proliferation in the zona reticularis was only slight.

Cell proliferation returned to the level in control animals at 4 weeks. It is assumed that the regulative process following ovariectomy takes place directly in the adrenal cortex but it is possible that steroid metabolism in the liver is of importance. No relationship between the3H-TdR labelling index (LI) and the mitotic index (MI) was observed.

Key words

Adrenal cortex Cell proliferation Ovariectomised rats 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Brenner RM (1963) Radioautographic studies with tritiated thymidine of cell migration in the mouse adrenal after a carbon tetrachloride stress. Am J Anat 112:81–96PubMedCrossRefGoogle Scholar
  2. Bury HPR, Crane WAJ (1965) Effect of age and hormonal state on the numbers of deoxyribonucleic acid synthesizing nuclei in rat adrenal cortex. Nature [Lond] 205:301–302CrossRefGoogle Scholar
  3. Cowdry EV (1942) Ageing of individual cells. In: Cowdry EV (ed) Problems of ageing. The Williams and Wilkins Company, Baltimore, p 626Google Scholar
  4. Crane WAJ, Dutta LP (1963) The utilisation of tritiated thymidine for deoxyribonucleic acid synthesis by the lesions of experimental hypertension in rats. J Pathol Bact 86:83–97CrossRefGoogle Scholar
  5. Dhom G, Stöcker E (1964) Autoradiographische Studien über die DNS-Synthese im Hypophysenvorderlappen der Ratte. Experienta 20:384–385CrossRefGoogle Scholar
  6. Dhom G, Stöcker E (1965) Die DNS-Synthese im Hypophysenvorderlappen der Ratte bei funktioneller Belastung. In: Klein E (ed) Wachstumshormon und Wachstumsstörungen. Das Cushing-Syndrom. Springer, Berlin, p 298Google Scholar
  7. Dhom G, von Seebach HB, Stephan G (1971) Der Geschlechtsdimorphismus der Nebennierenrinde der Ratte. Z Zellforsch 116:119–135PubMedCrossRefGoogle Scholar
  8. Diderholm H, Hellman B (1960) The cell renewal in the rat adrenals studied with tritiated thymidine. Acta Pathol Microbiol Scand (A) 49:82–88Google Scholar
  9. Edwards JL, Klein RE (1961) Cell renewal in adult mouse tissue. Am J Pathol 38:437–4153PubMedGoogle Scholar
  10. Ford JK, Young RW (1963) Cell proliferation and displacement in the adrenal cortex of young rats injected with tritiated thymidine. Anat Rec 146:125–137PubMedCrossRefGoogle Scholar
  11. Kreibig G (1973) Veränderungen an der Nebennierenrinde der Ratte nach neonataler Kastration. Inauguraldissertation, Medizinische Fakultät der Universität des Saarlandes, Homburg/SaarGoogle Scholar
  12. Machemer R, Oehlert W (1964) Autoradiographische Untersuchungen über den physiologischen Zellumsatz und die gesteigerte Zellneubildung der Nebenniere der ausgewachsenen Ratte nach Behandlung mit ACTH. Endokrinologie 46:77–91PubMedGoogle Scholar
  13. Malendowicz LK, Jachimowicz LB (1982) Sex differences in adrenocortical structure and function. XI. Autoradiographic studies on cell proliferation and turnover in the adrenal cortex of the male and female rat and its dependence on testosterone and estradiol. Cell Tissue Res 227:651–657PubMedCrossRefGoogle Scholar
  14. Messier B, Leblond CP (1960) Cell proliferation and migration as revealed by radioautography after injection of thymidine-H3 into male rats and mice. Am J Anat 106:247–285PubMedCrossRefGoogle Scholar
  15. Neuss J (1983) Einfluß der Kastration auf die Proliferation der Nebennierenrinde bei weiblichen Ratten. Inauguraldissertation, Medizinische Fakultät der Universität des Saarlandes, Homburg/SaarGoogle Scholar
  16. Noltenius H, Kemperman H, Oehlert W (1964) Histoautoradiographische Untersuchungen mit3H-Thymidin zur experimentellen Nierenvergrößerung nach unilateraler Nephrektomie bei Ratten. Naturwissenschaften 51:63–64CrossRefGoogle Scholar
  17. Pappritz G, Keazor H, Ueberberg H (1977) Estrous Cycle-Controlled Cell Proliferation in the Adrenal Cortex of Female Rats. Cell Tiss Res 184:269–279CrossRefGoogle Scholar
  18. Parkes AS (1945) The adrenal-gonad relationship. Physiol Rev 25:203–254Google Scholar
  19. Schriefers H (1969) Regulationen des Steroidhormonstoffwechsels in der Leber. In: Wannagat L (ed) Leber, Endokrinium und Wasserhaushalt. Georg Thieme Verlag, Stuttgart, p 34Google Scholar
  20. Schriefers H, Hoff HG, Ghraf R, Ockenfels H (1972) Geschlechts- und altersabhängige Entwicklung des Aktivitätsmusters von Enzymen des Steroidhormon-Stoffwechsels in der Rattenleber nach neonatalem Eingriff in die sexuelle Differenzierung. Acta Endocrinol [Copenh] 69:789–800Google Scholar
  21. Schultze B (1968) Die Orthologie und Pathologie des Nucleinsäure- und Eiweißstoffwechsels der Zelle im Autoradiogramm. In: Altmann HW, Büchner F, Cottier H, Holle G, Letterer E, Masshoff W, Meessen H, Roulet F, Scifert G. Siebert G, Studer A (eds) Stoffwechsel und Feinstruktur der Zelle. Hdbch allg Pathol, vol 2 part 5, p 466Google Scholar
  22. Schultze B, Oehlert W (1960) Autoradiographic investigation of incorporation of3H-thymidine into cells of the rat and mouse. Science 131:737–738PubMedCrossRefGoogle Scholar
  23. Stöcker E, Altmann HW (1964) Die DNS-Synthese in Leberparenchymzellen und Gallengangsepithelien von normalen und mit Thioacetamid behandelten Ratten. Naturwissenschaften 51:15CrossRefGoogle Scholar
  24. Stöcker E, Kabus K, Dhom G (1965) Autoradiographische Studien über die DNS-Synthese in der Nebennierenrinde von Ratten. Z Zellforsch 65:206–210Google Scholar
  25. Ueberberg H, Stöcker E, Städtler F (1970) Zur cellulären Nucleinsäure- und Protein-Synthese der Nebennierenrinde von Ratten nach Dexamethason-Applikation. Virchows Arch [Cell Pathol] 6:97–106Google Scholar
  26. Ueberberg H, Muff HG, Trieb G (1984) Autoradiographic investigation of cell proliferation in the adrenal cortex of castrated rats under the influence of oestradiol. Experientia 40:1348–1385CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Heinz Ueberberg
    • 1
  • Jutta Neuss
    • 1
  • Gernot Trieb
    • 1
  1. 1.Department of Experimental PathologyDr. Karl Thomae GmbHBiberach/RißFederal Republic of Germany

Personalised recommendations