Ultrastructural localization of hCG in the placenta and in testicular germ cell tumors
- 11 Downloads
Human chorionic gonadotrophin(hCG)-producing cells in the human placenta and in testicular germ cell tumors have been investigated by ultrastructural immunohistochemistry using labeled Fab’fragments of anti-hCG-α and anti-hCG-β. Most reaction products were demonstrated in the rough endoplasmic reticulum (rER) and perinuclear spaces of syncytiotrophoblastic giant cells (STGC) occurring in embryonal carcinomas and in syncytial cells observed in choriocarcinomas and in normal placenta. The rER of placental and choriocarcinomatous syncytial cells was highly developed and invariably showed a positive reaction. However, the rER of STGCs varied both with regard to its degree of development and its immunoreactivity with hCG. Localization of hCG in the Golgi apparatus and in cytoplasmic granules was indefinite.
Mononuclear cells reacting positively with anti-hCG-α or -β were also demonstrated. Cytotrophoblast-like cells which failed to react with anti-hCG-α or -β were noted adjacent to STGCs and the significance of these cells is discussed.
Key wordsPlacenta Germ cell tumors Human chorionic gonadotrophin Immunohistochemistry Ultrastructure
Unable to display preview. Download preview PDF.
- Heyderman E, Neville AM (1976) Syncytiotrophoblasts in malignant testicular tumors. Lancet 103Google Scholar
- Isobe Y, Chen ST, Nakane PK, Brown WR (1977) Studies on translocation of immunoglobulins across intestinal epithelium. 1. Improvements in the peroxidase-labeled antibody method for application to study of human intestinal mucosa. Acta Histochem Cytochem 10:161–171Google Scholar
- Kurman RJ, Scardino PT, Mclntire KR, Waldmann TA, Javadpour N (1977) Cellular localization of alpha-fetoprotein and human chorionic gonadotropin in germ cell tumors of the testis using an indirect immunoperoxidase technique. A new approach to classification utilizing tumor markers. Cancer 40:2136–2151PubMedCrossRefGoogle Scholar
- Okumura H, Sudo T, Fujisawa M, Namba S, Matsushima S (1976) Purification of subunits from human chorionic gonadotropin and radioimmunoassay. Folia Endocrinol Jpn 52:105–113 [in Japanese]Google Scholar
- Padykula HA (1983) The human placenta. In: Weiss L (ed) Histology. Cell and tissue biology. Elsevier Science Publishing, New York, p 976Google Scholar
- Williams CA, Chase MW, Eds (1967) Methods in immunology and immunochemistry. Academic Press, New York, vol 1, pp 422–423Google Scholar
- Wilson MB, Nakane PK (1978) Immunofluorescence and related staining technique. Elsevier, North-Holland, Biomedical Press, Amsterdam-Oxford-New York, pp 215–224Google Scholar