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Science in China Series C: Life Sciences

, Volume 48, Supplement 1, pp 76–81 | Cite as

Correlations between the ages ofAlnus host species and the genetic diversity of associated endosymbioticFrankia strains from nodules

  • Dai Yumei 
  • Chenggang Zhang
  • Zhi Xiong
  • Zhongze Zhang
Article

Abstract

Nodule samples were collected from four alder species:Alnus nepalensis, A. sibirica, A. tinctoria andA. mandshurica growing in different environments on Gaoligong Mountains, Yunnan Province of Southwest China and on Changbai Mountains, Jilin Province of Northeast China. PCR-RFLP analysis of the IGS betweennifD andnifK genes was directly applied to unculturedFrankia strains in the nodules. A total of 21 restriction patterns were obtained. TheFrankia population in the nodules ofA. nepalensis had the highest genetic diversity among all fourFrankia populations; by contrast, the population in the nodules ofA. mandshurica had the lowest degree of divergence; the ones in the nodules ofA. sibirica andA. tinctoria were intermediate. A dendrogram, which was constructed based on the genetic distance between the restriction patterns, indicated thatFrankia strains fromA. sibirica andA. tinctoria had a close genetic relationship.Frankia strains fromA. nepalensis might be the ancestor ofFrankia strains infecting otherAlnus species. From these results and the inference of the ages ofAlnus host species, it is deduced that there was a co-evolution betweenAlnus and its microsymbiontFrankia in China.

Keywords

Frankia Alnus genetic diversity co-evolution 

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Copyright information

© Science in China Press 2005

Authors and Affiliations

  • Dai Yumei 
    • 1
    • 2
  • Chenggang Zhang
    • 1
  • Zhi Xiong
    • 3
  • Zhongze Zhang
    • 1
  1. 1.Institute of Applied EcologyChinese Academy of SciencesShenyangChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina
  3. 3.School of Forest ResourcesSouthwest Forestry CollegeKunmingChina

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