Virchows Archiv B

, Volume 25, Issue 1, pp 345–353 | Cite as

A lymphocyte chalone assay using lymphocyte colony growth in agar capillaries

  • Reinhard Maschler
  • H. Rainer Maurer


Using a recently developed method of culturing T-lymphocyte colonies in agar-containing capillary tubes, the capacity of three different lymphoid extracts with lymphocyte chalone (LC) activity to inhibit colony growth was demonstrated. Sephadex fractions from a calf spleen extract were tested on the colony growth of granulocytic cells and PHA-stimulated T-lymphocytes as well as on the in vitro uptake of3H-thymidine by bone marrow and ConA-and LPS-stimulated mouse spleen cells. The data strongly suggest that it is only the combination of several different assay systems applied to the same fractions that permits a clear-cut determination of a specific lymphocyte proliferation inhibitor like LC.

Key words

Lymphocyte chalone assay Agar colonies-capillaries 


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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Reinhard Maschler
    • 1
  • H. Rainer Maurer
    • 1
  1. 1.Pharmazeutisches Institut der Freien Universität BerlinBerlin 33

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