Summary
Using a recently developed method of culturing T-lymphocyte colonies in agar-containing capillary tubes, the capacity of three different lymphoid extracts with lymphocyte chalone (LC) activity to inhibit colony growth was demonstrated. Sephadex fractions from a calf spleen extract were tested on the colony growth of granulocytic cells and PHA-stimulated T-lymphocytes as well as on the in vitro uptake of3H-thymidine by bone marrow and ConA-and LPS-stimulated mouse spleen cells. The data strongly suggest that it is only the combination of several different assay systems applied to the same fractions that permits a clear-cut determination of a specific lymphocyte proliferation inhibitor like LC.
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Maschler, R., Maurer, H.R. A lymphocyte chalone assay using lymphocyte colony growth in agar capillaries. Virchows Arch. B Cell Path. 25, 345–353 (1977). https://doi.org/10.1007/BF02889444
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DOI: https://doi.org/10.1007/BF02889444