Virchows Archiv B

, 15:1 | Cite as

The reticulum cell and the reticular cell in the mouse popliteal lymph node

An electron microscopic autoradiographic study


The popliteal lymph nodes of normal and sensitized mice have been examined after administration of a carbohydrate antigen (pneumococcal polysaccharide). In some animals125I-labelled antigen was used to study antigen distribution; in others the administration of unlabelled antigen was followed by tritiated thymidine to study proliferation. The lymph nodes were examined by electron microscopy and E. M. autoradiography. A group of cells is present, here defined as reticulum cells, some of which may be macrophage precursors and some of which bind antigen in the germinal centres of sensitised animals (“dendritic reticular cells”).


Reticulum Cell Tritiated Thymidine Thymidine Uptake Popliteal Lymph Node Granular Endoplasmic Reticulum 


  1. Caro, L. G., Tubergen, R. F., van: High resolution autoradiography methods. J. Cell Biol.15, 173–188 (1962)PubMedCrossRefGoogle Scholar
  2. Carr, I.: The fine structure of the mammalian lymphoreticular system. Int. Rev. Cytol.27, 283–248 (1970)PubMedCrossRefGoogle Scholar
  3. Carr, I.: The fine structure of microfibrils and microtubules in macrophages and other lymphoreticular cells in relation to cytoplasmic movement. J. Anat. (Lond.)112, 383–389 (1972)Google Scholar
  4. Cohn, Z. A., Hirsch, J. G., Fedorko, M. E.: The in vitro differentiation of mononuclear phagocytes (IV). J. exp. Med.123, 747–756 (1966a)PubMedCrossRefGoogle Scholar
  5. Cohn, Z. A., Hirsch, J. G., Fedorko, M. E.: The in vitro differentiation of mononuclear phagocytes (V). J. exp. Med.123, 757–766 (1966b)PubMedCrossRefGoogle Scholar
  6. Fahey, J. L., Humphrey, J. H.: Antibodies with differing molecular size in mice. Immunology5, 104–109 (1962)PubMedGoogle Scholar
  7. Furth, R., van, Cohn, Z. A., Hirsch, J. G., Humphrey, J. H., Spector, W. G., Langevoort, H.L.: The mononuclear phagocyte system: a new classification of macrophages, monocytes and their precursor cells. Bull Wld Hlth Org.46, 845–852 (1972)Google Scholar
  8. Greenwood, F. C., Hunter, W. M., Glover, J. S.: The preparation of I-131 labelled human growth hormone of high specific radioactivity. Biochem. J.89, 114–123 (1963)PubMedGoogle Scholar
  9. Heidelberger, M., Kendall, F. E., Scherp, H. W.: The specific polysaccharides of types I, II, and III pneumococcus. J. exp. Med.64, 559–575 (1963)CrossRefGoogle Scholar
  10. Humphrey, J. H.: Personal Communication (1969)Google Scholar
  11. Maruyama, K., Masuda, T.: Antigen distribution and structure of the germinal center in lymph nodes of guinea pigs sensitised with ferritin. Ann. Rep. Virus Inst. Kyoto.8, 50–61 (1965)Google Scholar
  12. Maximow, A. A.: In: Special cytology (E. V. Cowdry, ed.) vol. 2., p. 711–77.0 New York: Hoeber 1932Google Scholar
  13. Mori, Y., Lennert, K.: Electron microscopic atlas of lymph node cytology and Pathology. Berlin-Heidelberg-New York: Springer 1969Google Scholar
  14. North, R. J.: The mitotic potential of fixed phagocytes in the liver as revealed during the developmen of cellular immunity. J. exp. Med.130, 315–323 (1969)PubMedCrossRefGoogle Scholar
  15. Nossal, G. J., V., Abbot, A., Mitchell, J., Lummus, Z.: Antigens in immunity XV. Ultrastructural features of antigen capture in primary and secondary lymphoid follicles. J. exp. Med.127, 277–289 (1968)PubMedCrossRefGoogle Scholar
  16. Spector, W. G.: The granulomatous exudate. Int. Rev. exp. Path.8, 1–59 (1969)PubMedGoogle Scholar
  17. Stuart, A. D., Davidson, A. E.: The human reticular cell; Morphology and cytochemistry. J. Path.103, 41–47 (1971)PubMedCrossRefGoogle Scholar
  18. Volkman, A., Gowans, J. L.: The origin of macrophages from the bone marrow in the rat. Brit. J. exp. Path.46, 662–670 (1965)Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • I. Carr
    • 1
  1. 1.Department of PathologyUniversity and Weston Park HospitalSheffield

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