Summary
The technique of labelled mitoses was used to investigate the cell proliferation kinetics in two human neoplasms, one a malignant melanoma and one a fibrosarcoma, transplanted to and grown serially in the athymic nude mutant (ANM) mouse. The experimental data obtained correlated well with a theoretical percentage labelled mitoses curve based on the assumption that the time spent by a cell in each of the phases M, G1 S and G2 is described by four independent log-normal distributions. However, no unique second wave was defined by the experimental results. This means that only the deductions made about the duration of the G2 and S phases are reliable. The median duration of these two phases was found to be 8 and 18 h and 8 and 16 h for the malignant melanoma and the fibrosarcoma, respectively. By comparing these results with results published on cell cycle studies of transplantable animal tumors and human tumors in situ, it is concluded that the cell cycle parameters of a human tumor grown in the ANM mouse are close to those of the same tumor in the donor patient.
Similar content being viewed by others
References
Barrett, J.C.: A mathematical model of the mitotic cycle and its application to the interpretation of percentage labelled mitosis data. J. Nat. Cancer Inst.37, 443–450 (1966)
Brown, J.M.: Exploitation of kinetic differences between normal and malignant cells. Radiology114, 189–197 (1975)
Bröyn, T.: Kinetics of cell proliferation and cell loss in the peripheral and central parts of Walker tumors growing in rats and nude mice. Virchows Arch. B Cell Path.18, 181–191 (1975)
Crowther, D.: Blood and neoplastic diseases. A rational approach to the chemotherapy of human malignant disease. I. Br. Med. J.4, 156–159 (1974)
Denekamp, J., Kallman, R.F.: In vitro and in vivo labelling of animal tumours with titrated thymidine. Cell Tissue Kinet.6, 217–227 (1973)
Denekamp, J., Thomlinson, R.H.: The cell proliferation kinetics of four experimental tumours after acute X-irradiation. Cancer Res.31, 1279–1284 (1971)
Flanagan, S.P.: “Nude”, a new hairless gene with pleiotropic effects in the mouse. Genet. Res. (Camb.)8, 295–309 (1966)
Frindel, E., Malaise, E., Tubiana, M.: Cell proliferation kinetics in five human solid tumours. Cancer22, 611–620 (1968)
Isaacson, J.H., Cattanach, B.M.: Report. Mouse News Letter27, 31 (1962)
Mendelsohn, M.L.: The kinetics of tumor cell proliferation. In: Cellular Radiation Biology, pp. 498–513. Baltimore: Williams & Wilkins Inc. 1965
Peckham, M.J., Steel, G.G.: Cell kinetics in reticulum cell sarcoma. Cancer29, 1724–1728 (1972)
Pickard, R.G., Cobb, L.M., Steel, G.G.: The growth kinetics of xenografts of human colorectal tumours in immune deprived mice. Br. J. Cancer31, 36–45 (1975)
Povlsen, C.O., Fialkow, P.J., Klein, E., Klein, G., Rygaard, J., Wiener, F.: Growth and antigenic properties of a biopsy-derived Burkitt’s Lymphoma in thymusless (nude) mice. Int. J. Cancer11, 30–39 (1973)
Povlsen, CO., Rygaard, J.: Heterotransplantation of human adenocarcinomas of the colon and rectum to the mouse mutant nude. A study of nine consecutive transplantations. Acta path. microbiol. scand. Section A.79, 159–169 (1971)
Povlsen, C.O., Rygaard, J.: Heterotransplantation of human epidermoid carcinomas to the mouse mutant nude. Acta path. microbiol. scand. Section A.80, 713–717 (1972)
Rygaard, J.: Thymus and self. Immunobiology of the mouse mutant nude. Copenhagen: F.A.D.L. 1973
Shirakawa, S., Luce, J.K., Tannock, I., Frei, E.: Cell proliferation in human melanoma. J. Clin. Invest.49, 1188–1199 (1970)
Simpson-Herren, L., Blow, J.G., Brown, P.H.: The mitotic cycle of Sarcoma 180. Cancer Res.28, 724–726 (1968)
Steel, G.G.: The cell cycle in tumours: An examination of data gained by the technique of labelled mitosis. Cell Tissue Kinet.5, 87–100 (1972)
Steel, G.G., Hanes, S.: The technique of labelled mitosis: Analysis by automatic curve-fitting. Cell Tissue Kinet.4, 93–105 (1971)
Terasima, R., Tolmach, L.J.: X-ray sensitivity and DNA synthesis in synchronous populations of HeLa cells. Science140, 490–492 (1963)
Terz, J.J., Curutchet, P., Lawrence, W., Jr.: Analysis of the cell kinetics of human solid tumours. Cancer28, 1100–1110 (1971)
Visfeldt, J., Povlsen, C.O., Rygaard, J.: Chromosome analysis of human tumours following heterotransplantation to the mouse mutant nude. Acta path. microbiol. scand. Section A.80, 169–176 (1972)
Young, R.C., De Vita, V.T.: Cell cycle characteristics of human solid tumours in vivo. Cell Tissue Kinet.3, 285–290 (1970)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rofstad, E.K., Brustad, T. & Kaalhus, O. Cell proliferation kinetics in two human tumors grown in athymic nude mice. Virchows Arch. B Cell Path. 24, 219–225 (1977). https://doi.org/10.1007/BF02889281
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02889281