Virchows Archiv B

, 18:35 | Cite as

Cell kinetics of mouse urinary bladder epithelium

I. Circadian and age variations in cell proliferation and nuclear DNA content
  • Tore Farsund


The urinary bladder epithelium contains nuclei with diploid, tetraploid and octoploid DNA content. A study in mice has been performed on mitotic activity, DNA synthesis and ploidy. The study has confirmed previous findings of an extremely low mitotic activity and a LI of 0.40±0.04%. The results indicate a higher DNA synthesis during the night as compared to the day.

The relative numbers of diploid-, tetraploid-and octoploid cells have been estimated by micro-flow fluorometry. The mean values of these different classes were diploid: 39.40±1.14%, tetraploid: 53.27 ±1.00% and octoploid: 4.41 ±0.22%. Hexaploid DNA values with a mean of 2.51 ±0.13% were found and the significance of hexaploid pulses is discussed.

The different ploidy classes changed during the neonatal period with a decrease of the diploid class and an increase of the tetraploid class. A reduction of the octoploid class about 4 weeks after birth was seen. The distribution of the different classes became stabilized around the second month.


Labelling Index Relative Number Diploid Cell Circadian Variation Relative Fluorescence Intensity 
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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Tore Farsund
    • 1
  1. 1.Institute of PathologyUniversity of OsloRikshospitalet

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