Cell kinetics of mouse urinary bladder epithelium
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The urinary bladder epithelium contains nuclei with diploid, tetraploid and octoploid DNA content. A study in mice has been performed on mitotic activity, DNA synthesis and ploidy. The study has confirmed previous findings of an extremely low mitotic activity and a LI of 0.40±0.04%. The results indicate a higher DNA synthesis during the night as compared to the day.
The relative numbers of diploid-, tetraploid-and octoploid cells have been estimated by micro-flow fluorometry. The mean values of these different classes were diploid: 39.40±1.14%, tetraploid: 53.27 ±1.00% and octoploid: 4.41 ±0.22%. Hexaploid DNA values with a mean of 2.51 ±0.13% were found and the significance of hexaploid pulses is discussed.
The different ploidy classes changed during the neonatal period with a decrease of the diploid class and an increase of the tetraploid class. A reduction of the octoploid class about 4 weeks after birth was seen. The distribution of the different classes became stabilized around the second month.
KeywordsLabelling Index Relative Number Diploid Cell Circadian Variation Relative Fluorescence Intensity
- Berkhan, E.: DNS-Messung von Zellen aus Vaginalabstrichen. Ärztl. Lab.18, 77–79 (1972)Google Scholar
- Clayson, D. B., Cooper, E. H.: Cancer of the urinary tract. Advanc. Cancer Res.13, 271–381 (1970)Google Scholar
- Cooper, E. H.: The biology of bladder cancer. Ann. roy. Coll. Surg. Engl.51, 1–16 (1972)Google Scholar
- Dittrich, W., Göhde, W.: Impulsfluorometrie bei Einzelzellen in Suspension. Z. Naturforsch.24b, 350–361 (1969)Google Scholar
- Farsund, T.: Preparation of bladder mucosa cells for micro-flow fluorometry. Virchows Arch. Abt.B 16, 35–42 (1974)Google Scholar
- Himes, M., Hoffman, J., Pollister, A. W., Post, J.: Origin of polyploid nuclei in rat livers during regeneration following carbon tetrachloride poisoning. J. Mt Sinai Hosp.24, 935–938 (1957)Google Scholar
- Laerum, O. D., Maurer, H. R.: Proliferation kinetics of myelopoietic cells and macrophages in diffusion chambers after treatment with granulocyte extracts (chalone). Virchows Arch. Abt.B 14, 293–305 (1973)Google Scholar
- Levi, P. E., Cowen, D. M., Cooper, E. H.: Induction of cell proliferation in the mouse bladder by 4-ethylsulphonyl-naphthalene-l-sulphonamide. Cell Tiss. Kinet.2, 249–262 (1969)Google Scholar
- Martin, B. F.: Cell replacement and differentiation in transitional epithelium: a histological and autoradiographic study of the guinea-pig bladder and ureter. J. Anat. (Lond.)112, 433–455 (1972)Google Scholar
- Tvermyr, E. M. F.: Circadian rhythms in hairless mouse epidermal DNA-synthesis as measured by double labelling with H3-Thymidine (H3Tdr). Virchows Arch. Abt.B 11, 43–54 (1972)Google Scholar