Virchows Archiv B

, Volume 35, Issue 1, pp 213–228 | Cite as

Experimental intrahepatic cholestasis

A comparative physiopathological, ultrastructural, and cytochemical study of different types
  • H. Loosli
  • D. Gardiol
  • A. Gautier


Currently little is known about the role of the various constituents of the biliary pole of the hepatocyte in the process of cholestasis. In an attempt to delineate their respective roles we used three approaches-physiopathology, ultrastructure and cytochemistry- to study three types of chemically induced intrahepatic cholestasis: sodium taurolithocholate (NaTL-)cholestasis, phalloidin (P-)cholestasis, and cholestasis caused by the successive action of these two agents (mixed cholestasis).

We compared variations in the biliary flow rate with changes in the plasma levels of certain enzymes, and the ultrastructural alterations revealed by certain cytochemical reactions. According to our experimental findings, NaTL-cholestasis seems to be associated primarily with membrane modification and release of a considerable amount of non-esterified cholesterol; P-cholestasis appears to be associated chiefly with a change in the cytoskeleton of the pericanalicular ectoplasm and moderate release of free cholesterol; whereas mixed cholestasis reveals intermediary characteristics.

Special attention was given to the demonstration, by cytochemical reaction, of free cholesterol under the electron and fluorescence microscope.

Key words

Rat liver Experimental cholestasis Physiopathology Ultrastructure Cytochemistry 


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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • H. Loosli
    • 1
  • D. Gardiol
    • 1
  • A. Gautier
    • 2
  1. 1.Institut de PathologieCentre Hospitalier Universitaire VaudoisLausanne 11 CHUVSwitzerland
  2. 2.Centre de Microscopie électroniqueUniversité de LausanneLausanneSwitzerland

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