Epithelial cell kinetics in the descending colon of the rat
- 14 Downloads
Epithelial cell kinetics were investigated in the descending colon of the rat. The number of cells per crypt was found to be approximately 625, with 33 cells per cell column and 19 cell columns per crypt circumference. The growth fraction of the colonic crypt was 0.42, and proliferating cells were situated largely in the lower half of the crypt. The cell cycle time was 50.5 h, with values for the G1, S and G2 phases of 40.0, 7.6 and 2.9 h respectively. Cell migration studies showed that it took 60–72 h for a cell to migrate from the upper border of the proliferative cell compartment in the crypt to the luminal surface of the colon.
Data were also obtained from continuous labelling with tritiated thymidine and from studying the circadian rhythm of proliferative activity, which suggest that the cells in the bottom of the crypt may constitute a separate, more slowly cycling (stem)cell compartment.
Key wordsColon Kinetics Thymidine
- Leblond, C.P., Cheng, H.: Stem cells in the small intestine of the mouse. In: Stem cells, Cairnie, A.B., Lala, P.K., Osmond, D.G. (eds.), pp. 7–31. New York: Academic Press 1976Google Scholar
- Pötten, C.S.: Small intestinal crypt stem cells. In: Stem cells, Cairnie, A.B., Lala, P.K., Osmond, D.G. (eds.), pp. 79–84. New York: Academic Press 1976Google Scholar
- Rijke, R.P.C.: Control mechanisms of cell proliferation in intestinal epithelium. Ph.D. thesis, Erasmus Univ. Rotterdam (1977)Google Scholar
- Steel, G.G.: The measurement of the intermitotic period. In: The cell cycle in development and differentiation, Balls, M., Billet, F.S. (eds.), pp. 13–29. Cambridge: University Press 1973Google Scholar
- Sunter, J.P., Wright, N.A., Appleton, D.R.: Cell population kinetics in the epithelium of the colon of the male rat. Virchows Arch. B Cell Path.26, 275–287 (1978)Google Scholar
- Tutton, P.J.M., Barkla, D.H.: Cell proliferation in the descending colon of dimethylhydrazine treated rats and in demethylhydrazine induced adenocarcinomata. Virchos Arch. B Cell Path.21, 147–160(1976)Google Scholar