Virchows Archiv B

, 30:85 | Cite as

Epithelial cell kinetics in the descending colon of the rat

  • R. P. C. Rijke
  • H. M. Plaisier
  • N. J. Langendoen


Epithelial cell kinetics were investigated in the descending colon of the rat. The number of cells per crypt was found to be approximately 625, with 33 cells per cell column and 19 cell columns per crypt circumference. The growth fraction of the colonic crypt was 0.42, and proliferating cells were situated largely in the lower half of the crypt. The cell cycle time was 50.5 h, with values for the G1, S and G2 phases of 40.0, 7.6 and 2.9 h respectively. Cell migration studies showed that it took 60–72 h for a cell to migrate from the upper border of the proliferative cell compartment in the crypt to the luminal surface of the colon.

Data were also obtained from continuous labelling with tritiated thymidine and from studying the circadian rhythm of proliferative activity, which suggest that the cells in the bottom of the crypt may constitute a separate, more slowly cycling (stem)cell compartment.

Key words

Colon Kinetics Thymidine 


  1. Al-Dewachi, H.S., Wright, N.A., Appleton, D.R., Watson, A.J.: The cell cycle time in the rat jejunal mucosa. Cell Tissue Kinet.7, 587–594 (1974)PubMedGoogle Scholar
  2. Bertalanffy, F.D.: Mitotic rates and renewal times of the digestive tract epithelia in the rat. Acta Anat.40, 130–148 (1960)PubMedCrossRefGoogle Scholar
  3. Burholt, D.R., Schultze, B., Maurer, W.: Mode of growth of the jejunal crypt cells of the rat. An autoradiographic study using double labelling with3H-and14C-thymidine in lower and upper parts of the crypts. Cell Tissue Kinet.9, 107–117 (1976)PubMedGoogle Scholar
  4. Cairnie, A.B., Bentley, R.E.: Cell proliferation studies in the intestinal epithelium of the rat. Hyperplasia during lactation. Exp. Cell Res.46, 428–440 (1967)PubMedCrossRefGoogle Scholar
  5. Cairnie, A.B., Lamerton, L.F., Steel, G.G.: Cell proliferation studies in the intestinal epithelium of the rat. I. Determination of the kinetic parameters. Exp. Cell Res.39, 528–538 (1965)PubMedCrossRefGoogle Scholar
  6. Chang, W.W.L., Leblond, C.P.: Renewal of the epithelium in the descending colon of the mouse. I. Presence of three cell populations: vacuolated-columnar, mucous and argentaffin. Amer. J. Anat.131, 73–100 (1971a)PubMedCrossRefGoogle Scholar
  7. Chang, W.W.L., Leblond, C.P.: Renewal of the epithelium in the descending colon of the mouse. II. Renewal of argentaffin cells. Am. J. Anat.131, 101–110 (1971b)PubMedCrossRefGoogle Scholar
  8. Chang, W.W.L., Andler, N.J.: Renewal of the epithelium in the descending colon of the mouse. IV. Cell population kinetics of vacuolated-columnar and mucous cells. Am. J. Anat.144, 39–56 (1975)PubMedCrossRefGoogle Scholar
  9. Galjaard, H., Bootsma, D.: The regulation of cell proliferation and differentiation in intestinal epithelium. II. A quantitative histochemical and autoradiographic study after low doses of X-irradiation. Exp. Cell Res.58, 79–92 (1969)PubMedCrossRefGoogle Scholar
  10. Hagemann, R.F., Stragand, J.J.: Fasting and refeeding -cell kinetic response of jejunum, ileum and colon. Cell Tissue Kinet.10, 3–14 (1977)PubMedGoogle Scholar
  11. Hopper, A.F., Wannemacher, R.W., McGovern, P.A.: Cell population changes in the intestinal epithelium of the rat following starvation and protein-depletion. Proc. Soc. Exp. Biol. Med.128, 695–698 (1968)PubMedGoogle Scholar
  12. Kovacs, L., Potten, C.S.: An estimation of proliferative population size in stomach, jejunum and colon of DBA-2 mice. Cell Tissue Kinet.6, 125–134 (1973)PubMedGoogle Scholar
  13. Leblond, C.P., Cheng, H.: Stem cells in the small intestine of the mouse. In: Stem cells, Cairnie, A.B., Lala, P.K., Osmond, D.G. (eds.), pp. 7–31. New York: Academic Press 1976Google Scholar
  14. Lesher, S., Fry, R.J.M., Kohn, H.I.: Age and the generation time of the mouse duodenal epithelial cell. Exp. Cell Res.24, 334–343 (1961)PubMedCrossRefGoogle Scholar
  15. Lipkin, M.: Proliferation and differentiation of gastrointestinal cells. Physiol. Rev.53, 891–915 (1973)PubMedGoogle Scholar
  16. Lipkin, M., Quastler, H.: Cell population kinetics in the colon of the mouse. J. Clin. Invest.41, 141–146 (1962a)PubMedCrossRefGoogle Scholar
  17. Lipkin, M., Quastler, H.: Cell retention and incidence of carcinoma in several portions of the gastrointestinal tract. Nature194, 1198–1199 (1962b)PubMedCrossRefGoogle Scholar
  18. Maskens, A.P.: Histogenesis and growth pattern of 1,2-dimethyl-hydrazine-induced rat colon adenocarcinoma. Cancer Res.36, 1585–1592 (1976)PubMedGoogle Scholar
  19. Messier, B., Leblond, C.P.: Cell proliferation and migration as revealed by radioautography after injection of thymidine-H3 into male rats and mice. Am. J. Anat.106, 247–285 (1960)PubMedCrossRefGoogle Scholar
  20. Nabeyama, A.: Presence of cells combining features of two different cell types in the colonic crypts and pyloric glands of the mouse. Am. J. Anat.142, 471–484 (1975)PubMedCrossRefGoogle Scholar
  21. Pötten, C.S.: Small intestinal crypt stem cells. In: Stem cells, Cairnie, A.B., Lala, P.K., Osmond, D.G. (eds.), pp. 79–84. New York: Academic Press 1976Google Scholar
  22. Pötten, C.S.: Extreme sensitivity of some intestinal crypt cells toX and γ irradiation. Nature269, 518–521 (1977)PubMedCrossRefGoogle Scholar
  23. Potten, C.S., Al-Barwari, S.E., Hume, W.J., Searle, J.: Cicadian rhythms of presumptive stem cells in three different epithelia of the mouse. Cell Tissue Kinet.10, 557–568 (1977)PubMedGoogle Scholar
  24. Potten, C.S., Kovacs, L., Hamilton, E.: Continuous labelling studies on mouse skin and intestine. Cell Tissue Kinet.7, 271–283 (1974)PubMedGoogle Scholar
  25. Quastler, H., Sherman, F.G.: Cell population kinetics in the intestinal epithelium of the mouse. Exp. Cell Res.17, 420–438 (1959)PubMedCrossRefGoogle Scholar
  26. Rijke, R.P.C.: Control mechanisms of cell proliferation in intestinal epithelium. Ph.D. thesis, Erasmus Univ. Rotterdam (1977)Google Scholar
  27. Steel, G.G.: The measurement of the intermitotic period. In: The cell cycle in development and differentiation, Balls, M., Billet, F.S. (eds.), pp. 13–29. Cambridge: University Press 1973Google Scholar
  28. Steel, G.G., Hanes, S.: The technique of labelled mitoses-analysis by automatic curve fitting. Cell Tissue Kinet.4, 93–105 (1971)PubMedGoogle Scholar
  29. Sunter, J.P., Wright, N.A., Appleton, D.R.: Cell population kinetics in the epithelium of the colon of the male rat. Virchows Arch. B Cell Path.26, 275–287 (1978)Google Scholar
  30. Tutton, P.J.M., Barkla, D.H.: Cell proliferation in the descending colon of dimethylhydrazine treated rats and in demethylhydrazine induced adenocarcinomata. Virchos Arch. B Cell Path.21, 147–160(1976)Google Scholar
  31. Wimber, D.R., Lamerton, L.F.: Cell population studies on the intestine of continuously irradiated rats. Radiat. Res.18, 137–146 (1963)CrossRefGoogle Scholar
  32. Wright, N.A., Al-Dewachi, H.S., Appleton, D.R., Watson, A.J.: Cell population kinetics in the rat jejunal crypt. Cell Tissue Kinet.8, 361–368 (1975)PubMedGoogle Scholar
  33. Wright, N.A., Watson, A., Morley, A., Appleton, D., Marks, J., Douglas, A.: The measurement of cell production rates in the crypts of Lieberkühn. An experimental and clinical study. Virchows Arch. A Path. Anat. Histol.364, 311–323 (1974)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • R. P. C. Rijke
    • 1
  • H. M. Plaisier
    • 1
  • N. J. Langendoen
    • 1
  1. 1.Department of Cell Biology and GeneticsErasmus University RotterdamThe Netherlands

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