Virchows Archiv B

, 28:351 | Cite as

Cell junctions in dyserythropoiesis

  • B. Frisch
  • S. M. Lewis


Erythroid cell junctions have previously been reported in some cases of dyserythropoietic anaemia. This study has demonstrated that they are a relatively common occurrence in dyserythropoiesis. They range from apposition of cellular membranes with preservation of the intercellular space, to membrane confluence, to interdigitation and septate-like junctions. By special staining with colloidal lanthanum, ruthenium red and tannic acid, together with studies of the effect of proteolytic enzymes, their structural composition has been identified and it was confirmed that they are not artefact. The role of cell junctions in dyserythropoiesis is discussed.

Key words

Dyserythropoiesis Congenital dyserythropoietic anaemia Cell junctions Erythroblasts Ultrastructure 


  1. Azarnia, R., Larsen, W.J.: Intercellular communication and cancer. Intercellular communications. (W.C. de Mello, ed.) pp. 145–172. New York, London: Plenum Press 1977Google Scholar
  2. Bennet, S., Leblond, C.P., Haddad, A.: Migration of glycoprotein from the Golgi apparatus to the surface of various cell types as shown by radioautography after labelled sucrose injection into rats. J. Cell Biol.60, 258–284 (1974)CrossRefGoogle Scholar
  3. Breton-Gorius, J., Flandrin, G., Daniel, M.T., Chevalier, J., Lebenau, M., Sanel, F.T.: Septate-like junctions in abnormal erythroblasts: Cytochemical, ultrastructural and freeze-etch studies. Virchows Arch. B Cell Path.18, 165–180 (1975)Google Scholar
  4. Bronn, D.G., Kibbey, W.E., Baba, N., Weber, E.G., Hart, R.W., Minton, J.P.: Intercellular bridge formation in breast cancer cells treated with 5-fluorouracil. J. Cell Biol.70, 237 a (1976)Google Scholar
  5. Crick, F.H.C., Lawrence, P.A.: Compartments and polyclones in insect development. Science189, 340–347 (1975)PubMedCrossRefGoogle Scholar
  6. Douglas, W.H.J., Ripley, R.C., Ellis, R.A.: Enzymatic digestion of desmosome and hemi-desmosome plaques performed on ultrathin sections J. Cell Biol.44, 211–214 (1970)PubMedCrossRefGoogle Scholar
  7. Durand, R.E., Sutherland, R.M.: Effect of intercellular contact on repair of radiation damage. Exp. Cell Res.71, 75–80 (1972)PubMedCrossRefGoogle Scholar
  8. Flandrin, P.G., Daniel, M.-Th., Breton-Gorius, J., Brouet, J.-C., Bernard, J.: Ilôt érythroblastique anormal dû au développement de junctions intercellulaires (synastèse érythroblastique). Nouv. Rev. Franç. Hemat.14, 161–180 (1974)PubMedGoogle Scholar
  9. Frisch, B., Broeckaert-van Orshoven, A.: Ultrastructure of normal and abnormal erythropoiesis. Dyserythropoiesis. (S.M. Lewis and R.L. Verwilghen, eds.), pp. 271–314. London, New York, San Francisco: Academic Press 1977Google Scholar
  10. Frisch, B., Lewis, S.M., Sherman, D.: The ultrastructure of dyserythropoiesis in aplastic anaemia. Brit. J. Haemat.,29, 545–552 (1975)PubMedCrossRefGoogle Scholar
  11. Frisch, B., Lewis, S.M., Swan, M., Intercellular contacts between erythroid precursors in the bone marrow in dyserythropoiesis. Brit. J. Haemat.33, 469–475 (1976)PubMedCrossRefGoogle Scholar
  12. Furcht, L.T., Scott, R.E.: Cell contact mediated changes in membrane structure. J. Cell Biol.59, 106 a (1973)Google Scholar
  13. Futaesaku, Y., Mizuhira, V., Nakamura, H.: The new fixation method using tanic acid for electron microscopy and some observations of biological specimens. Proc. of Fourth Int. Congress of Histochem. Cytochem, Kyoto. pp. 155–156 (1972)Google Scholar
  14. Gillula, N.B., Branton, D., Satia, P.: The septate junction. A structural basis for intercellular coupling. Proc. Nat. Acad. Sci.67, 213–220 (1970)CrossRefGoogle Scholar
  15. Gillula, N.B., Reeves, O.R., Steinbeck, A.: Metabolic coupling, ionic coupling and cell contacts. Nature235, 262–265 (1972)CrossRefGoogle Scholar
  16. Lewis, S.M., Verwilghen, R.L.: Dyserythropoiesis: definition, diagnosis and assessment. Dyserythro-poiesis. (S.M. Lewis, R.L. Verwilghen, eds.), pp. 3–20. London, New York, San Francisco: Academic Press 1977Google Scholar
  17. Luft, J.H.: Ruthenium red and violet: Fine structural localisation in animal tissues. Anat. Rec.171, 368–416 (1971)Google Scholar
  18. Nicholson, G.L., Singer, S.J.: The distribution and assymmetry of mammalian cell surface saccharides utilising ferritin conjugated plant agglutinins as specific saccharide stains. J. Cell. Biol.60, 236–248 (174)Google Scholar
  19. Pederson, D.C., Sheridan, J.D., Johnson, R.G.: Evidence for nucleotide transfer during junctional formation in culture. J. Cell Biol.70, 338 a (1976)Google Scholar
  20. Pitts, J.D., Finbow, M.E.: Junctional permeability and its consequences. Intercellular communication (W.C. de Mello, ed.), pp. 61–86. New York, London: Plenum Press 1977Google Scholar
  21. Revel, J.P., Karnovsky, M.J.: Hexagonal assay of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol.23, C7–12 (1967)CrossRefGoogle Scholar
  22. Staehelin, L.A.: Structure and function of intercellular junctions. Int. Rev. Cytol.39, 191–283 (1974)PubMedCrossRefGoogle Scholar
  23. Wagner, R.C.: The effect of tannic acid on electron images of capillary endothelial cell membranes. J. Ultra. Res.57, 132–139 (1976)CrossRefGoogle Scholar
  24. Weiss, L.: Some biophysical aspects of cell contacts in metastases. Cellular membranes and tumor cell behaviour. (E.F. Walborg, ed.), pp. 361–382. Baltimore: Williams and Wilkins 1975Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • B. Frisch
    • 1
  • S. M. Lewis
    • 1
  1. 1.Department of HaematologyRoyal Postgraduate Medical SchoolLondonUK

Personalised recommendations