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Tumour cell interactions in vitro

Evidence for the occurrence of tumour cells within other tumour cells

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Virchows Archiv B

Summary

After addition of dibutyryl cAMP to spinner flask cultures of JB-l-E tumour cells an increase in the number of examples of emperipolesis was observed. Ruthenium red staining of the cells revealed a conspicuous staining of the cell membrane of the outer cell, while that of the inner cell was unstained. The differences in staining properties of the cell membranes of the outer and inner cells involved in emperipolesis and apparently viable inner cells make it evident that coexistence of cells —one within another—is possible.

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References

  • Behnke, O.: Electron microscopical observations on the surface coating of human blood platelets. J. Ultrastruct. Res.24, 51–69 (1968)

    Article  PubMed  CAS  Google Scholar 

  • Bichel, P., Barfod, N.M., Jakobsen, A.: Employment of synchronized cells and flow microfluorometry in investigations on the JB-1 ascites tumour chalones. Virchows Arch. B Cell Path.19, 127–133 (1975)

    CAS  Google Scholar 

  • Burns, E.R.: Tumor cell-tumor emperipolesis. Exp. Cell Res.48, 229–231 (1967)

    Article  PubMed  CAS  Google Scholar 

  • Chemnitz, J., Bichel, P.: Tumour cell —tumour cell emperipolesis studied by transmission electron microscopy. Exp. Cell Res.82, 319–324 (1973)

    Article  PubMed  CAS  Google Scholar 

  • Chemnitz, J., Skaaring, P., Bichel, P.: Increasing occurrence of tumour cell — tumour cell emperipolesis in the regenerating JB-1 ascites tumour. Z. Krebsforsch.84, 89–96 (1975)

    Article  CAS  Google Scholar 

  • Haemmerli, G., Felix, H., Sträuli, P.: Motility of L 5222 rat leukemia cells in the flattened state. Evidence against emperipolesis. Virchows Arch. B Cell Path.24, 165–178 (1977)

    CAS  Google Scholar 

  • Humble, J.G., Jayne, W.H.W., Pulvertaft, J.V.: Biological interaction between lymphocytes and other cells. Brit. J. Haemat.2, 283–294 (1956)

    Article  PubMed  CAS  Google Scholar 

  • Knyrim, K., Paweletz, N.: Cell interactions in a “bilayer” of tumor cells. A scanning electron microscope study. Virchows Arch. B Cell Path.25, 309–325 (1977)

    CAS  Google Scholar 

  • Margolick, J.B., Sherwin, R.P.: Lymphocytocidal lymphocyte trapping by human lymph node cells: A tissue culture-ultrastructural study. In Vitro12, 407–417 (1976)

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Winslow Institute of Human Anatomy, University of Odense, Campusvej 55, DK-5230, Odense M, Denmark

    J. Chemnitz & P. Skaaring

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  1. J. Chemnitz
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  2. P. Skaaring
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Chemnitz, J., Skaaring, P. Tumour cell interactions in vitro. Virchows Arch. B Cell Path. 28, 87–91 (1978). https://doi.org/10.1007/BF02889059

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  • DOI: https://doi.org/10.1007/BF02889059

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