Tumour cell interactions in vitro
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After addition of dibutyryl cAMP to spinner flask cultures of JB-l-E tumour cells an increase in the number of examples of emperipolesis was observed. Ruthenium red staining of the cells revealed a conspicuous staining of the cell membrane of the outer cell, while that of the inner cell was unstained. The differences in staining properties of the cell membranes of the outer and inner cells involved in emperipolesis and apparently viable inner cells make it evident that coexistence of cells —one within another—is possible.
Key wordsTumour cell interaction Emperipolesis Ruthenium red Spinner culture
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- Bichel, P., Barfod, N.M., Jakobsen, A.: Employment of synchronized cells and flow microfluorometry in investigations on the JB-1 ascites tumour chalones. Virchows Arch. B Cell Path.19, 127–133 (1975)Google Scholar
- Haemmerli, G., Felix, H., Sträuli, P.: Motility of L 5222 rat leukemia cells in the flattened state. Evidence against emperipolesis. Virchows Arch. B Cell Path.24, 165–178 (1977)Google Scholar
- Knyrim, K., Paweletz, N.: Cell interactions in a “bilayer” of tumor cells. A scanning electron microscope study. Virchows Arch. B Cell Path.25, 309–325 (1977)Google Scholar