Abstract
The decomposition of glycine in soil was studied by the continuous flow method. Glycine solution was added continuously to soil samples of different weights, i.e. soil columns of different heights. It was found that the extent of glycine mineralization was related to the weight of the soil. Glycine was nitrified most effectively in the soil sample weighing 30 g., in which 65.8% of the added (91.6% of the retained) glycine nitrogen was oxidized to nitrites and nitrates. No steady state was observed in the rate of nitrite and nitrate formation. The rate of nitrification rose at first, in relation to the weight of the soil, but fell after reaching the maximum. The factor limiting the rate of nitrification was the adsorption of ammonium nitrogen in the soil. By using soil samples of different weights and heights it was found possible to localize the process of ammonia release and the oxidation of ammonia and nitrites in the soil column and to influence the ratio of ammonification and nitrification or of the oxidation of ammonium ions and nitrites.
Abstract
Методом непрерывного проточного культивирования изучалось разложение глицина в почве. Раствор глицина непрерывно подводили к образцам почвы различного веса, т. е. к столбцам почвы различной высоты. Было установлено, что степень минерализации глицина пропорциональна весу почвы. Нитрификация глицина оказалась наиболее эффективной в образце почвы весом в 30 г, в котором 65,8% прибавленного, т. е. 91.6% адсорбированного азота в глипине окислялись и превращались в нитриты и нитраты. Что касается скорости образования нитритов и нитратов, не наблюдалосьсостояния равновесия. В начале скорость нитрификации повышалась в соответствии с повышением веса почвы, но после достижения максимума она понижалась. Фактором, лимитирующим скорость нитрификации, была адсорбция аммиачного азота в почве. Путем применения образцоб почвы различного веса и различной толщины слоя удалось локализовать процесс освобождения аммиака и окисления аммиака и нитритов в столбце почвы, а также регулировать соотношение между аммонификацией и нитрификацией или между окислением ионов аммония и нитрита.
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References
Bremmer, J. M.:Studies on soil organic matter. I. J. Agric. Res. 39: 183, 1949.
Bremner, J. M.:The amino acid composition of protein material in soil. Biochem. J. 47: 538, 1950.
Bremner, J. M.:Studies on soil humic acids. II. J. Agr. Sci. 48: 352, 1957.
Dadd, C. C., Fowden, L., Pearsall, W. H.:An investigation of the free amino acids in organic soil types using paper partition chromatography. J. Soil Sci. 4: 69, 1953.
Greenwood, D. J., Lees, H.:Studies on the decomposition of amino acids in soils, I, II, III. Plant & Soil 7: 253, 1956; 12: 69, 175, 1960.
Ivashkevich, T. M., Kuprevich, V. F., Shcherbakova, T. A.:Changes in the composition of free amino acids in soil during the vegetation period. Doklady AN SSSR 7: 704, 1963. (in Russian). (Ивашкевич, Т. М., Купревич, В. Ф., Шербакова, Т. А.: Доклады AH CCCP 7: 704: 1963.
Kojima, R. T.:Soil organic nitrogen. I, II. Soil Sci. 64: 157, 245, 1947.
Lees, H., Quastel, J. H.:Biochemistry of nitrification in soil. I. II.. Biochem. J. 40: 803, 815, 1946.
Macura, J.,Continuous flow method in soil microbiology. I. Fol. microbiol. 6: 328, 1961.
Macura, J., Kunc, F.:Continuous flow method in soil microbiology. II. Fol. microbiol. 6: 398, 1961.
Macura, J., Kunc, F.:Continuous flow method in soil microbiology. III. Fol. microbiol. 10: 36, 1965.
Macura, J., Szolnoki, J., Kunc, F., Vančura, V.:Decomposition of glucose continuously added to the soil. Fol. microbiol. 10: 44, 1965.
Paul, E. A., Schmidt, E. L.:Formation of free amino acids in rhizosphere and nonrhizosphere soil. Soil Sci. Soc. Amer. Proc. 25: 359, 1961.
Pavel, L., Koloušek, J., Šmatlák, V.:Huminové látky. II. Sborník ČSAZV 27A: 207, 1954.
Payne, T. M. B., Rouatt, J. W., Katznelson, H.:Detection of free amino acids in soil. Soil Sci. 82: 521, 1956.
Putman, H. G., Schmidt, E. L.:Studies on the free amino acid fraction of soils. Soil Sci. 87: 22, 1959.
Quastel, J. H., Scholefield, P. G.:Influence of organic compounds on nitrification in soil. Nature 164: 1068, 1949.
Quastel, J. H., Scholefield, P. G.:Biochemistry of soil nitrification. Bact. Rev. 15: 1: 1951.
Simonart, P., Mayaudon, J.:Étude de la décomposition de la matière organique dans le sol au moyen de carbon radioactif. II. Plant & Soil 9: 381, 1958.
Sørensen, H.:Studies on the decomposition of C 14-labelled barley straw in soil. Soil Sci. 95: 45, 1963.
Sowden, F. J.:Estimation of amino acids in soil hydrolyzates by the Moore and Stein method. Soil Sci. 80: 181, 1955.
Sowden, F. J.:Distribution of amino acids in selected horizons of soil profiles. Soil Sci. 82: 491, 1956.
Stevenson, F. J.:Ion exchange chromatography of amino acids in soil hydrolysates. Soil Sci. Soc. Amer. Proc. 18: 373, 1954.
Stojanovic, J., Alexander, M.:Effect of inorganic nitrogen on nitrification. Soil Sci. 86: 208, 1958.
Tombesi, L.:Paper chromatography and Lees's percolation technique applied to the study of amino acids transformations in soil (In Italian). Ann. Sper. Agrar. Roma 7: 1219, 1953.
Vančura, V.:Root exudates of plants. I. Plant & Soil. 21: 231, 1964.
Vančura, V., Macura, J., Szolnoki, J.:Products of glucose metabolism in the soil. 8th Intern. Congr. Soil Sci., Bucarest 1964.
Wheeler, B. E. J.:The conversion of amino acids in soils. II. Plant & Soil 19: 219, 1963.
Wheeler, B. E. J., Yemm, E. W.:The conversion of amino acids in soils. I. Plant & Soil 10: 49, 1958.
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Macura, J., Kunc, F. Continuous flow method in soil microbiology. Folia Microbiol 10, 115–124 (1965). https://doi.org/10.1007/BF02888913
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DOI: https://doi.org/10.1007/BF02888913